pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CCDC88C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CCDC88C-FLAG           7296 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..4009
                     /codon_start=1
                     /label=CCDC88C
                     /translation="ELGERHGDMLKRKAELEEREKVLTTEREALQQEQRTNALAMGENQ
                     RLRGELDRVNFLHHQLKGEYEELHAHTKELKTSLNNAQLELNRWQARFDELKEQHQTMD
                     ISLTKLDNHCELLSRLKGNLEEENHHLLSQIQLLSQQNQMLLEQNMENKEQYHEEQKQY
                     IDKLNALRRHKEKLEEKIMDQYKFYDPPPKKKNHWIGAKALVKLIKPKKEGSRERLKST
                     VDSPPWQLESSDPASPAASQPLRSQAENPDTPALGSNCAEERDAHNGSVGKGPGDLKPK
                     RGSPHRGSLDRTDASTDLAMRSWPSELGSRTCSTSATTTAPSNSTPIARHPGRTKGYNS
                     DDNLCEPSLEFEVPNHRQYVSRPSSLESSRNTSSNSSPLNLKGSSEQLHGRSESFSSED
                     LIPSRDLATLPREASTPGRNALGRHEYPLPRNGPLPQEGAQKRGTAPPYVGVRPCSASP
                     SSEMVTLEEFLEESNRSSPTHDTPSCRDDLLSDYFRKASDPPAIGGQPGPPAKKEGAKM
                     PTNFVAPTVKMAAPTSEGRPLKPGQYVKPNFRLTEAEAPPSVAPRQAQPPQSLSLGRPR
                     QAPVPPASHAPASRSASLSRAFSLASADLLRASGPEACKQESPQKLGAPEALGGRETGS
                     HTLQSPAPPSSHSLARERTPLVGKAGSSCQGPGPRSRPLDTRRFSLAPPKEERLAPLHQ
                     SATAPAIATAGAGAAAAGSGSNSQLLHFSPAAAPAARTKPKAPPRSGEVATITPVRAGL
                     SLSEGDGVPGQGCSEGLPAKSPGRSPDLAPHPGRALEDCSRGSVSKSSPASPEPGGDPQ
                     TVWYEYGCV"
     misc_feature    1936..1941
                     /label=SDS
     misc_feature    2114..2119
                     /label=SDS
     misc_feature    2853..2858
                     /label=SDS
     misc_feature    3321..3326
                     /label=SDS
     misc_feature    3600..3605
                     /label=SDS
     CDS             4016..4081
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4085..4516
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4229..4234
                     /label=SDS
     repeat_region   4559..4699
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4559..4688
                     /label=AAV2 ITR
     rep_origin      4774..5229
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5511..5615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5616..6476
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6647..7235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggagc tcggggagag
     1561 gcacggtgac atgctgaagc gcaaggcgga gctggaggag cgggagaagg tcttgaccac
     1621 tgagcgagag gcgctgcagc aggagcagag gacaaacgcc ctcgccatgg gcgagaacca
     1681 gaggctgcgg ggcgagctgg acagggtcaa tttcctgcac caccagctga agggggagta
     1741 cgaggagctg cacgcccaca ccaaggagct gaaaacctca ctgaacaacg cgcagctgga
     1801 gctcaaccgc tggcaggccc gcttcgacga gctgaaggag cagcaccaga ccatggacat
     1861 ctcgctgacc aagctggaca accactgtga gctgctctcc cgtctcaagg ggaacttgga
     1921 ggaagaaaat catcacctcc tgagccagat ccagctgttg agccagcaga accagatgct
     1981 tctggagcag aacatggaga acaaggagca gtaccatgag gagcagaagc agtacataga
     2041 caaattaaat gccttacgaa gacataagga aaagctggaa gaaaaaatca tggatcaata
     2101 caagttctat gatcctcctc caaagaagaa gaaccactgg attggagcca aagccttagt
     2161 caaactcatc aaaccaaaga aagagggttc gagggaacgc ttaaaatcca ccgtggacag
     2221 ccctccctgg cagctggagt cctcagaccc cgcctcgccg gcggcctctc agccgctcag
     2281 atcacaggcc gagaaccccg acacccccgc actgggctcc aactgtgcag aagagcgcga
     2341 cgcccacaac gggtctgtgg ggaaaggccc tggggatcta aaaccaaagc gaggctcccc
     2401 acacagaggc agccttgacc gcacagatgc ctccaccgat ctggccatga ggtcctggcc
     2461 ctcggagctg ggctcccgga cttgctcaac ttcagccacc actacagccc cttccaactc
     2521 cacccccatc gcccggcacc caggccgcac caaaggctat aactcagatg acaacctctg
     2581 tgagccatcc ctggagtttg aggtccccaa ccacaggcag tacgtgtcgc ggccaagtag
     2641 cttagagagc agtagaaaca catccagcaa cagctcacct cttaacctaa aaggctcctc
     2701 cgagcagctc catggccggt ctgagagctt cagcagcgaa gacctgatcc ccagcaggga
     2761 cctggccact ttgccccggg aagccagcac accgggacgc aacgccctcg gccgccacga
     2821 gtaccccttg cctcggaacg ggcctctccc acaggagggt gcccagaaga ggggcacagc
     2881 ccctccctac gtcggagtgc ggccctgctc ggcctccccc agcagtgaga tggtcacctt
     2941 ggaggagttc ctggaggaga gcaaccgcag ctcccccacc catgacactc ccagttgccg
     3001 ggatgacctg ctgagtgact acttccgaaa ggccagcgat cccccagcca tcggaggcca
     3061 accaggacca cctgccaaga aagaaggggc caagatgccc accaactttg tggcccccac
     3121 cgtcaaaatg gccgccccca cctcggaggg gaggccgctg aagcccgggc agtacgtaaa
     3181 gccaaacttc agactgactg aggccgaggc cccacccagc gtggccccga gacaggccca
     3241 gcctccccag agcctgtctc tgggcagacc ccggcaggct ccggtgcccc cagcttccca
     3301 tgcacctgcc agccgcagtg cctccttgag ccgggccttc agcctggcct cagctgacct
     3361 tctccgggcc agcgggccag aggcctgcaa acaggagtcc cctcagaagc tgggggctcc
     3421 tgaggcctta gggggcagag agacaggcag ccacaccctg caaagccccg caccccccag
     3481 ctcccatagc ctggcccggg agcggacccc acttgtggga aaggctggca gctcctgtca
     3541 gggcccaggt ccccgcagcc ggccgctgga cacgaggcgc ttctccctgg ctcccccaaa
     3601 ggaggagagg ctggcccccc tgcatcagtc tgccacagcc cccgccattg ccactgcagg
     3661 tgctggtgct gctgctgctg gcagtggcag caactcccag ctcctgcact tctcacctgc
     3721 tgcagccccg gctgccagga ccaagcccaa ggcgccccca cgctcagggg aggtggccac
     3781 catcacccct gtccgggcag ggctcagcct ctcagaggga gacggggtcc cggggcaggg
     3841 ctgcagtgag gggcttccgg ccaagagccc aggtcggtct cccgatttgg ctccccaccc
     3901 cggccgggcc ctggaggact gcagtcgagg gagcgtctca aagagcagtc cggcctcccc
     3961 ggagcccggc ggggatccgc agaccgtgtg gtatgagtac ggctgtgtgt gtacagacta
     4021 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     4081 gtaactcgag ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4141 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4201 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
     4261 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4321 actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4381 atctagcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4441 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     4501 gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag
     4561 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4621 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4681 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4741 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4801 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     4861 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4921 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4981 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5041 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5101 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     5161 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     5221 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5281 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5341 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5401 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5461 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5521 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5581 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5641 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5701 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5761 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5821 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5881 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5941 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6001 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6061 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6121 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6181 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6241 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6301 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6361 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6421 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6481 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6541 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6601 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6661 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6721 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6781 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6841 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6901 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6961 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7021 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7081 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7141 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7201 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7261 ggttcctggc cttttgctgg ccttttgctc acatgt
//