| 质粒名称: | pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 85424 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR SIN |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 慢病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | anti-Her2_4D5-7_synNotch |
| 启动子 | PGK |
| 克隆方法: | Gibson克隆 |
| 5' 测序引物 | gcttcaaaagcgcacgtct |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64 was a gift from Wendell Lim (Addgene plasmid # 85424 ; http://n2t.net/addgene:85424 ; RRID:Addgene_85424) |
| 参考文献部分: | Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, Walker WJ, McNally KA, Lim WA. Cell. 2016 Oct 6;167(2):419-432.e16. doi: 10.1016/j.cell.2016.09.011. Epub 2016 Sep 29. 10.1016/j.cell.2016.09.011 PubMed 27693353 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64 11423 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64
synthetic Notch.
ACCESSION .
VERSION .
KEYWORDS pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11423)
AUTHORS Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH,
Walker WJ, McNally KA, Lim WA
TITLE Engineering T Cells with Customized Therapeutic Response Programs
Using Synthetic Notch Receptors.
JOURNAL Cell. 2016 Oct 6;167(2):419-432.e16. doi:
10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
PUBMED 27693353
REFERENCE 2 (bases 1 to 11423)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11423
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 64..93
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 2287..2436
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
primer_bind complement(2608..2628)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3026..3037)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3206..3439
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(3464..3482)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3465..3482)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3772..3791)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(4005..4024)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4124..4146
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4184..4202)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4270..4374
/gene="bla"
/label=AmpR promoter
CDS 4375..5235
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4593..4612)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5406..5994
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5895..5914
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6148..6165
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(6235..6255)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6240..6569
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6420..6555
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6482..6501
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 7703..7768
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 7898..7918
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 8343..8477
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8380..8399)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8434..8453
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 8645..9278
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 9325..9450
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 9947..10180
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 10694..10811
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 10871..11370
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(10906..10929)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 11303..11322
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 11418..4
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
ORIGIN
1 atggccctgc ctgttaccgc cctgttgctc cctcttgccc tgctgcttca cgccgctcgc
61 cctgagcaga aacttatatc agaggaagat ctggatattc agatgactca gtctcctagc
121 tctctctcag ctagtgtcgg ggacagagtg acaataacct gcagagcctc acaggacgtg
181 aatactgcgg tcgcctggta tcagcagaaa cctgggaagg cacccaaact gctcatttac
241 tccgctagct tcttggagag cggcgtccca tcacggttct ccgggagtcg cagcggcacc
301 gacttcaccc ttaccatcag ctctttgcag ccagaggatt ttgcaacgta ttattgccaa
361 cagcactaca ctactccccc aactttcgga caaggcacaa aagttgaaat taaaaggact
421 ggaagtacca gcggatccgg gaaaccgggt tccggtgaag gcagtgaggt gcaactggtt
481 gagagcggag gcggtctggt gcagcctggt ggttctctcc gcctcagctg cgctgcttcc
541 ggtttcaaca taaaagacac ttatattcac tgggtcaggc aggcaccagg caagggactc
601 gagtgggttg cgcgcattta tccgactaac gggtacacaa ggtacgctga tagtgtgaag
661 ggtagattta cgattagcgc cgacacctcc aaaaatacag catatctcca gatgaatagc
721 cttcgcgctg aagacacagc tgtatactac tgcagtagat ggggcggtga tggcttctac
781 gccatggacg tgtggggtca gggaactctg gtgaccgttt caagcggtag tatcctggac
841 tacagcttca caggtggcgc tgggcgcgac attcccccac cgcagattga ggaggcctgt
901 gagctgcctg agtgccaggt ggatgcaggc aataaggtct gcaacctgca gtgtaataat
961 cacgcatgtg gctgggatgg tggcgactgc tccctcaact tcaatgaccc ctggaagaac
1021 tgcacgcagt ctctacagtg ctggaagtat tttagcgacg gccactgtga cagccagtgc
1081 aactcggccg gctgcctctt tgatggcttc gactgccagc tcaccgaggg acagtgcaac
1141 cccctgtatg accagtactg caaggaccac ttcagtgatg gccactgcga ccagggctgt
1201 aacagtgccg aatgtgagtg ggatggccta gactgtgctg agcatgtacc cgagcggctg
1261 gcagccggca ccctggtgct ggtggtgctg cttccacccg accagctacg gaacaactcc
1321 ttccactttc tgcgggagct cagccacgtg ctgcacacca acgtggtctt caagcgtgat
1381 gcgcaaggcc agcagatgat cttcccgtac tatggccacg aggaagagct gcgcaagcac
1441 ccaatcaagc gctctacagt gggttgggcc acctcttcac tgcttcctgg taccagtggt
1501 gggcgccagc gcagggagct ggaccccatg gacatccgtg gctccattgt ctacctggag
1561 atcgacaacc ggcaatgtgt gcagtcatcc tcgcagtgct tccagagtgc caccgatgtg
1621 gctgccttcc taggtgctct tgcgtcactt ggcagcctca atattcctta caagattgag
1681 gccgtgaaga gtgagccggt ggagcctccg ctgccctcgc agctgcacct catgtacgtg
1741 gcagcggccg ccttcgtgct cctgttcttt gtgggctgtg gggtgctgct gtcccgcaag
1801 cgccggcgga tgaagctgct gagcagcatc gagcaggcct gtgacatctg ccggctgaag
1861 aaactgaagt gcagcaaaga aaagcccaag tgcgccaagt gcctgaagaa caactgggag
1921 tgccggtaca gccccaagac caagagaagc cccctgacca gagcccacct gaccgaggtg
1981 gaaagccggc tggaaagact ggaacagctg tttctgctga tcttcccacg cgaggacctg
2041 gacatgatcc tgaagatgga cagcctgcag gacatcaagg ccctgctgac cggcctgttc
2101 gtgcaggaca acgtgaacaa ggacgccgtg accgacagac tggccagcgt ggaaaccgac
2161 atgcccctga ccctgcggca gcacagaatc agcgccacca gcagcagcga ggaaagcagc
2221 aacaagggcc agcggcagct gacagtgtct gctgctgcag gcggaagcgg aggctctggc
2281 ggatctgatg ccctggacga cttcgacctg gatatgctgg gcagcgacgc cctggatgat
2341 tttgatctgg acatgctggg atctgacgct ctggacgatt tcgatctcga catgttggga
2401 tcagatgcac tggatgactt tgacctggac atgctcggat catgagatcc ttgacttgcg
2461 gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct
2521 gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
2581 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
2641 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
2701 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
2761 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
2821 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
2881 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
2941 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
3001 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
3061 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
3121 ctccctttgg gccgcctccc cttaagacca atgacttaca aggcagctgt agatcttagc
3181 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
3241 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
3301 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
3361 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
3421 gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa
3481 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata
3541 tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt
3601 aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc
3661 cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg
3721 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
3781 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
3841 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
3901 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
3961 tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat
4021 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
4081 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
4141 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
4201 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
4261 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
4321 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
4381 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
4441 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
4501 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
4561 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
4621 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
4681 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
4741 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
4801 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
4861 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
4921 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
4981 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
5041 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
5101 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
5161 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
5221 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
5281 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
5341 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
5401 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
5461 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
5521 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
5581 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
5641 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
5701 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
5761 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
5821 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
5881 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
5941 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
6001 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
6061 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
6121 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
6181 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg
6241 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
6301 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
6361 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
6421 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
6481 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
6541 gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata
6601 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
6661 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
6721 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
6781 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
6841 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt
6901 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
6961 acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
7021 tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc
7081 caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact
7141 atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat
7201 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc
7261 tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
7321 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
7381 acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt
7441 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt
7501 gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc
7561 gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg
7621 gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
7681 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
7741 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
7801 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
7861 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
7921 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
7981 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
8041 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
8101 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
8161 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
8221 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
8281 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
8341 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
8401 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
8461 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
8521 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
8581 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
8641 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
8701 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
8761 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
8821 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
8881 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
8941 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
9001 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
9061 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
9121 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
9181 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
9241 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
9301 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
9361 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
9421 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
9481 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
9541 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
9601 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
9661 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
9721 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
9781 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
9841 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
9901 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
9961 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
10021 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
10081 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
10141 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
10201 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
10261 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
10321 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
10381 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
10441 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
10501 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
10561 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
10621 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc caaatggcag
10681 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
10741 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
10801 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc gataagcttg
10861 atatcgaatt gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
10921 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
10981 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
11041 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
11101 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
11161 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
11221 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
11281 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
11341 ttctcctctt cctcatctcc gggcctttcg aattctcacg cgtcaagtgg agcaaggcag
11401 gtggacagtg gatcgccgcc acc
//
