| 质粒名称: | pBTK503 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 110616 |
| 存储实验室: | Jeffrey Barrick |
|
载体骨架基本信息 |
|
| 载体名称: | RSF1010 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBTK503 was a gift from Jeffrey Barrick (Addgene plasmid # 110616 ; http://n2t.net/addgene:110616 ; RRID:Addgene_110616) |
| 参考文献部分: | Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids. Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE. ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 10.1021/acssynbio.7b00399. Epub 2018 Apr 13. 10.1021/acssynbio.7b00399 PubMed 29608282 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBTK503 8790 bp ds-DNA circular SYN 10-AUG-2018
DEFINITION BTK assembled broad-host-range plasmid encoding GFP driven by CP25
promoter.
ACCESSION .
VERSION .
KEYWORDS pBTK503
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8790)
TITLE BTK plasmids
REFERENCE 2 (bases 1 to 8790)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 10, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8790
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 1661..1707
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(1716..2339)
/codon_start=1
/gene="tetR"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind 2247..2266
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
primer_bind complement(2379..2398)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2380..2398)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(2509..3369)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3132..3151
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3370..3474)
/gene="bla"
/label=AmpR promoter
CDS complement(4365..5204)
/codon_start=1
/gene="repA"
/product="replication protein A of the broad-host-range
plasmid RSF1010 from Salmonella enterica serovar
Typhimurium"
/label=RSF1010 RepA
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
CDS complement(5715..6686)
/codon_start=1
/gene="repB"
/product="replication protein B of the broad-host-range
plasmid RSF1010 from Salmonella enterica serovar
Typhimurium"
/label=RSF1010 RepB
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
rep_origin 8354..8748
/label=RSF1010 oriV
/note="replication origin of the broad-host-range plasmid
RSF1010 from Salmonella enterica serovar Typhimurium;
requires the RSF1010 RepA/B/C proteins for replication"
ORIGIN
1 tagcaacggc aggcatttcg gctgagggta aaagaacttt ccgctaagcg atagactgta
61 tgtaaacaca gtattgcaag gacgcggaac atgcctcatg tggcggccag gacggccagc
121 cgggatcggg atactggtcg ttaccagagc caccgacccg agcaaaccct tctctatcag
181 atcgttgacg agtattaccc ggcattcgct gcgcttatgg cagagcaggg aaaggaattg
241 ccgggctatg tgcaacggga atttgaagaa tttctccaat gcgggcggct ggagcatggc
301 tttctacggg ttcgctgcga gtcttgccac gccgagcacc tggtcgcttt cagaaatcaa
361 tctaaagtat atatgagtaa acttggtctg acaggcccct gaattcgcat ctagactgat
421 gagacgtggt agagccacaa acagccggta caagcaacga tctccaggac catctgaatc
481 atgcgcggat gacacgaact cacgacggcg atcacagaca ttaacccaca gtacagacac
541 tgcgacaacg tggcaattcg tcgcaataca acgctttggc agtttattct tgacatgtag
601 tgagggggct ggtataatca catagtactg ttatacagaa acagaggaga tattacatat
661 gagtaaagga gaagagcttt tcacaggagt tgtcccaatc ctcgtggaat tagacggtga
721 tgttaatggg cacaagttct ctgtcagtgg agagggtgaa ggcgacgcaa catatggcaa
781 gctgaccctt aaatttattt gcaccacggg taaactacct gttccatggc caacactggt
841 cactacgttc gggtatgggg ttcagtgctt tgcgcgctac ccagatcaca tgaaacagca
901 cgactttttc aagagtgcaa tgcccgaagg ctatgtacag gagagaacca tcttttttaa
961 ggatgacggc aactataaga cacgcgccga agtgaagttc gagggtgata cccttgttaa
1021 tagaatcgag ttaaagggta ttgactttaa ggaagatgga aatattttag gccacaaact
1081 ggaatataac tataactccc ataatgtgta cattatggcc gacaagcaaa agaacggtat
1141 caaggttaac ttcaagatca gacacaacat tgaggatgga agcgttcaac tagccgacca
1201 ttaccaacaa aacaccccaa ttggcgatgg gcctgtgctg ttaccagaca accattacct
1261 gtccactcaa tctgcccttt cgaaagatcc caacgaaaag cgcgaccaca tggtccttct
1321 tgagtttgtc acggctgctg ggattacaca cggcatggat gaactataca aataaatccg
1381 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag
1441 tttagggaaa gagcatttgt cagctggaaa tctgctcgtc agtggtgctc acactgacga
1501 atcatgtaca gatcataccg atgactgcct ggcgactcac aactaagcaa gacagccgga
1561 accagcgccg gcgaacacca ctgcatatat ggcatatcac aacagtccac gtctcaagca
1621 gttacagaga tgttacgaac cactagtgca ctgcagtaca caaaaaaccc ctcaagaccc
1681 gtttagaggc cccaaggggt tatgctaatg ctgctttaag acccactttc acatttaagt
1741 tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg ctctgcacct
1801 tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc agtagtaggt
1861 gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca acctaaagta
1921 aaatgcccca ctgcgctgag tgcatataat gcattctcta gtgaaaaacc ttgttggcat
1981 aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg tgtacctaaa
2041 tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt agcgttatta
2101 cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg gtgcctatct
2161 aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg ccaatacaat
2221 gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc ttcgattccg
2281 acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa acgagacatg
2341 agtatatctc cttctacaaa attatttcta gatctctccc ctatagtgag tcgtattaat
2401 ttccgagcgg ccgcgattat caaaaaggat cttcacctag atccttttaa attaaaaatg
2461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
2521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
2581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
2641 gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
2701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
2761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
2821 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
2881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
2941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
3001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
3061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
3121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
3181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
3241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
3301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
3361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
3421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
3481 tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa cgtgagtcag cctgccgcct
3541 tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga
3601 ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg
3661 cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg
3721 ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc
3781 gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca
3841 aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca
3901 gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct
3961 cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct
4021 ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg gcctcccact
4081 tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca
4141 ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc
4201 ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt
4261 ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac
4321 agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg
4381 ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca
4441 tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag
4501 cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc
4561 atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg
4621 gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac
4681 acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg
4741 gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg
4801 cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc
4861 aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg
4921 ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg
4981 ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct
5041 gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc gggcgacacc
5101 agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg tggcggcgct
5161 gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg cctcctttgc
5221 aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc gctctgagtt
5281 cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac
5341 gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg
5401 ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc gagaaacttg
5461 ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg
5521 ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt ataaccaaag
5581 ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt
5641 ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa caggcgtgag
5701 taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg
5761 ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag acccatgacc
5821 ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct
5881 gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg gctggccagc
5941 ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt gaccagcccg
6001 gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg
6061 ggcagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc
6121 tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc cttggattca
6181 cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg
6241 aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg
6301 ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg
6361 acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc ccggccctcg
6421 gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag accatgccgc
6481 tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg cttgagccat
6541 ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg gccggtggtg
6601 gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg tcggcctatg
6661 gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc gagccgtcct
6721 cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc accaccgtag
6781 gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc cgcgacgcgc
6841 tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac tctttggcca
6901 gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc gcctgcgcct
6961 cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt gtcgccatgc
7021 tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat ttcttcactc
7081 tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat gatctgggcg
7141 ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc ccggccttcc
7201 atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc ctgcgcctca
7261 agtgttctgt ggtcaatgcg ggcgtcgtgg ccagcccgct ctaatgcccg gttggcatgg
7321 tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc ttcggtcttc
7381 tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg agcggcgggc
7441 cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg gttctcgccg
7501 ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag gtgctgggcg
7561 aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag ggcaaattcg
7621 acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc ccagtagtcg
7681 gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac ttcatccatg
7741 tcgcgggcat acttgccttc gcgctggatg tagtcggcct tggccctggc cgattggccg
7801 cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc tcgctgttgc
7861 ttttgctttt cggctccatg caatggccct cggagagcgc accgcccgaa gggtggccgt
7921 taggccagtt tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag tagggtcggg
7981 attgccgccg ctgtgcctcc atgatagcct acgagacagc acattaacaa tggggtgtca
8041 agatggttaa ggggagcaac aaggcggcgg atcggctggc caagctcgaa gaacaacgag
8101 cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga acagcagcaa gagcgcaaga
8161 acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg aacagcagcg
8221 agtggccgga ggatcggctc atggcggcaa tggatgcgta ccttgaacgc gaccacgacc
8281 gcgccttgtt cggtctgccg ccacgccaga aggatgagcc gggctgaatg atcgaccgag
8341 acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa aggccgctaa
8401 agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt agcgggcttt
8461 gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag
8521 accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc
8581 gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt ttccaacacc
8641 ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt attgcagggg
8701 ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg acagttagta
8761 cgggagtgac gggcactggc tggcaatgtc
//
