| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001550 |
| 产品用途: | 3' AAVLINK plasmid for CADPS expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CADPS |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG 6470 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6470)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6470
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3183
/codon_start=1
/label=CADPS
/translation="VL*KLLSHSWKGF**KILLPQCHKRR*KQLSVNVWNRLR*STILG
SQSMPKSKRIKRMQKM*AG*SLLPKSLKIQYVLLN*SLKFFSKMRSTTQSHMLIKEKPL
RGGQI*WWSMRRRSCHSLQ*TWMQP*RCNLQTHGTVFHYFSC*MIFSVLTIICAMENFT
NTCKTCLPHLLLDMWI*WSPQLHNPFTGALSGSHGNQSRV*PVTYPM*TYPM*TFPKYQ
IYQLTSL*ASHKCLLFRHRHGWLLYMMRIMGQAPQKICFGNLTPFRPSFGTCTGLKKSL
ESTWNNG*S*WQVT*SNLVSKEPGLHLKLSCKKPVDQQIFESHSQYAPCLMLWLMPKLN
QQNFAAWKWAKSINTIQK*TN*LKKLLKK**HSWLQSSLLSWKECWQNYPDMTKGLCFL
LFCHLP*RQLPNMWMYLNPGWTWPTPT*LSSAILRMSCVIRSMRRCT*KGYLINGTTAP
*T*SAPG*RTGWTYSFIFIS*KH*LGW*RKPTEISDCKGSWTPP*TARPMKRSGTVSLW
RKPQHQ*VKVGDCRASA*RTAMRKTKKT"
misc_feature 1619..1624
/label=SDS
CDS 3190..3255
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3259..3690
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3403..3408
/label=SDS
repeat_region 3733..3873
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3733..3862
/label=AAV2 ITR
rep_origin 3948..4403
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4685..4789
/gene="bla"
/label=AmpR promoter
CDS 4790..5650
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5821..6409
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgc tttgaaagct
1561 actctctcac tcttggaaag ggttttgatg aaagatattg ttaccccagt gccacaagag
1621 gaggtaaaaa cagttatccg taaatgtctg gaacaggctg cgttagtcaa ctattctcgg
1681 ctctcagagt atgccaaaat cgaagagaat caaaaggatg cagaaaatgt aggccggtta
1741 atcactcctg ccaaaaagct tgaagataca atacgtcttg ctgaactagt cattgaagtt
1801 cttcagcaaa atgaggagca ccacgcagag ccacatgttg ataaaggaga agcctttgcg
1861 tggtggtcag atttaatggt ggagcatgcg gagacgttcc tgtcactctt tgcagtagac
1921 atggatgcag ccttagaggt gcaacctcca gacacatggg acagttttcc actatttcag
1981 ctgctgaatg attttctccg tactgactat aatttgtgca atggaaaatt tcacaaacac
2041 ctgcaagacc tgtttgcccc acttgttgtt agatatgtgg atctgatgga gtcctcaatt
2101 gcacaatcca ttcacagggg ctttgagcgg gagtcatggg aaccagtcaa gagtttaacc
2161 agtaacctac ccaatgtgaa cctacccaat gtgaaccttc ccaaagtacc aaatctacca
2221 gttaacatcc ctctaggcat cccacaaatg cctacttttt cggcaccgtc atggatggct
2281 gctatatatg atgcggataa tgggtcaggc acctcagaag atctgttttg gaaacttgac
2341 gcccttcaga ccttcattcg ggacctgcac tggcctgaag aagagtttgg aaagcacctg
2401 gaacaacggc tgaagttgat ggcaagtgac atgatcgaat cttgtgtcaa aagaaccagg
2461 attgcatttg aagttaagct gcaaaaaacc agtcgatcaa cagattttcg agtcccacag
2521 tcaatatgca ccatgtttaa tgttatggtt gatgccaaag ctcaatcaac aaaactttgc
2581 agcatggaaa tgggccaaga gcatcaatac cattcaaaaa tagacgaact aattgaagaa
2641 actgttaaag aaatgataac actcttggtt gcaaagttcg ttactatctt ggaaggagtg
2701 ctggcaaaat tatccagata tgacgaaggg actttgtttt cttcttttct gtcatttacc
2761 gtgaaggcag cttccaaata tgtggatgta cctaaacccg ggatggacgt ggccgacgcc
2821 tacgtgactt tcgtccgcca ttctcaggat gtcctgcgtg ataaggtcaa tgaggagatg
2881 tacatagaaa ggttatttga tcaatggtac aacagctcca tgaacgtgat ctgcacctgg
2941 ttgacggacc ggatggactt acagcttcat atttatcagt tgaaaacact aattaggatg
3001 gtaaagaaaa cctacagaga tttccgattg caaggggtcc tggactccac cttaaacagc
3061 aagacctatg aaacgatccg gaaccgtctc actgtggagg aagccacagc atcagtgagt
3121 gaaggtgggg gactgcaggg catcagcatg aaggacagcg atgaggaaga cgaagaagac
3181 gattgtacag actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
3241 gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt tgtgaaagat
3301 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
3361 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
3421 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
3481 ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg
3541 ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta
3601 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
3661 gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg cacgtgcgga
3721 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
3781 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
3841 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
3901 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
3961 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
4021 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
4081 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
4141 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
4201 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
4261 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
4321 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
4381 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
4441 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
4501 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
4561 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
4621 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
4681 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
4741 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
4801 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
4861 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
4921 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
4981 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
5041 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
5101 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
5161 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
5221 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
5281 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
5341 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
5401 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
5461 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
5521 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
5581 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
5641 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
5701 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
5761 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
5821 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
5881 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
5941 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
6001 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
6061 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
6121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
6181 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
6241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
6301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
6361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
6421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
