| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CACNA1C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001442 |
| 产品用途: | 3' AAVLINK plasmid for CACNA1C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CACNA1C |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CACNA1C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CACNA1C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CACNA1C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CACNA1C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CACNA1C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CACNA1C-FLAG 7107 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7107)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7107
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3820
/codon_start=1
/label=CACNA1C
/translation="VFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLAC
MPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTR
DWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRL
VSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLD
QVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDI
GPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQSDGRSAFPQ
TFTTQRPLHINKAGSSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRLP
RPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRCHSRESQAAMAGQEETSQDETYE
VKMNHDTEACSEPSLLSTEMLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRA
SFHLECLKRQKDRGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPP
ATPGSRGWPPQPVPTLRLEGVESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVS
LMVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEM
ESAADNILSGGAPQSPNGALLPFVNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRV
YVSSL"
misc_feature 1963..1968
/label=SDS
misc_feature 2433..2438
/label=SDS
misc_feature 2457..2462
/label=SDS
misc_feature 2495..2500
/label=SDS
misc_feature 2820..2825
/label=SDS
misc_feature 2889..2894
/label=SDS
misc_feature 3024..3029
/label=SDS
misc_feature 3096..3101
/label=SDS
misc_feature 3217..3222
/label=SDS
misc_feature 3780..3785
/label=SDS
CDS 3827..3892
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3896..4327
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4040..4045
/label=SDS
repeat_region 4370..4510
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4370..4499
/label=AAV2 ITR
rep_origin 4585..5040
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5322..5426
/gene="bla"
/label=AmpR promoter
CDS 5427..6287
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6458..7046
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt ttgggaaaat
1561 tgccctgaat gataccacag agatcaaccg gaacaacaac tttcagacct tcccccaggc
1621 cgtgctgctc ctcttcaggt gtgccaccgg ggaggcctgg caggacatca tgctggcctg
1681 catgccaggc aagaagtgtg ccccagagtc cgagcccagc aacagcacgg agggtgaaac
1741 accctgtggt agcagctttg ctgtcttcta cttcatcagc ttctacatgc tctgtgcctt
1801 cctgatcatc aacctctttg tagctgtcat catggacaac tttgactacc tgacaaggga
1861 ctggtccatc cttggtcccc accacctgga tgagtttaaa agaatctggg cagagtatga
1921 ccctgaagcc aagggtcgta tcaaacacct ggatgtggtg accctcctcc ggcggattca
1981 gccgccacta ggttttggga agctgtgccc tcaccgcgtg gcttgcaaac gcctggtctc
2041 catgaacatg cctctgaaca gcgacgggac agtcatgttc aatgccaccc tgtttgccct
2101 ggtcaggacg gccctgagga tcaaaacaga agggaaccta gaacaagcca atgaggagct
2161 gcgggcgatc atcaagaaga tctggaagcg gaccagcatg aagctgctgg accaggtggt
2221 gccccctgca ggtgatgatg aggtcaccgt tggcaagttc tacgccacgt tcctgatcca
2281 ggagtacttc cggaagttca agaagcgcaa agagcagggc cttgtgggca agccctccca
2341 gaggaacgcg ctgtctctgc aggctggctt gcgcacactg catgacatcg ggcctgagat
2401 ccgacgggcc atctctggag atctcaccgc tgaggaggag ctggacaagg ccatgaagga
2461 ggctgtgtcc gctgcttctg aagatgacat cttcaggagg gccggtggcc tgttcggcaa
2521 ccacgtcagc tactaccaaa gcgacggccg gagcgccttc ccccagacct tcaccactca
2581 gcgcccgctg cacatcaaca aggcgggcag cagccagggc gacactgagt cgccatccca
2641 cgagaagctg gtggactcca ccttcacccc gagcagctac tcgtccaccg gctccaacgc
2701 caacatcaac aacgccaaca acaccgccct gggtcgcctc cctcgccccg ccggctaccc
2761 cagcacagtc agcactgtgg agggccacgg gccccccttg tcccctgcca tccgggtgca
2821 ggaggtggcg tggaagctca gctccaacag gtgccactcc cgggagagcc aggcagccat
2881 ggcgggtcag gaggagacgt ctcaggatga gacctatgaa gtgaagatga accatgacac
2941 ggaggcctgc agtgagccca gcctgctctc cacagagatg ctctcctacc aggatgacga
3001 aaatcggcaa ctgacgctcc cagaggagga caagagggac atccggcaat ctccgaagag
3061 gggtttcctc cgctctgcct cactaggtcg aagggcctcc ttccacctgg aatgtctgaa
3121 gcgacagaag gaccgagggg gagacatctc tcagaagaca gtcctgccct tgcatctggt
3181 tcatcatcag gcattggcag tggcaggcct gagccccctc ctccagagaa gccattcccc
3241 tgcctcattc cctaggcctt ttgccacccc accagccaca cctggcagcc gaggctggcc
3301 cccacagccc gtccccaccc tgcggcttga gggggtcgag tccagtgaga aactcaacag
3361 cagcttccca tccatccact gcggctcctg ggctgagacc acccccggtg gcgggggcag
3421 cagcgccgcc cggagagtcc ggcccgtctc cctcatggtg cccagccagg ctggggcccc
3481 agggaggcag ttccacggca gtgccagcag cctggtggaa gcggtcttga tttcagaagg
3541 actggggcag tttgctcaag atcccaagtt catcgaggtc accacccagg agctggccga
3601 cgcctgcgac atgaccatag aggagatgga gagcgcggcc gacaacatcc tcagcggggg
3661 cgccccacag agccccaatg gcgccctctt accctttgtg aactgcaggg acgcggggca
3721 ggaccgagcc gggggcgaag aggacgcggg ctgtgtgcgc gcgcggggtc gaccgagtga
3781 ggaggagctc caggacagca gggtctacgt cagcagcctg tgtacagact acaaagacca
3841 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaactcga
3901 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
3961 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
4021 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
4081 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
4141 tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt
4201 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
4261 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
4321 tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
4381 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
4441 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
4501 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
4561 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
4621 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
4681 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
4741 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
4801 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
4861 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
4921 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
4981 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
5041 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
5101 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
5161 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
5221 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
5281 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
5341 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
5401 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
5461 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
5521 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
5581 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
5641 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
5701 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
5761 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
5821 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
5881 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
5941 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
6001 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
6061 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
6121 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
6181 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
6241 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
6301 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
6361 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
6421 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
6481 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
6541 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
6601 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
6661 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
6721 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
6781 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
6841 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
6901 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
6961 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
7021 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
7081 ccttttgctg gccttttgct cacatgt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CACNA1C-FLAG质粒载体应用举例
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