| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP10A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001487 |
| 产品用途: | 3' AAVLINK plasmid for ATP10A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ATP10A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP10A-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP10A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP10A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP10A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP10A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP10A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ATP10A-FLAG 7032 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7032)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7032
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3745
/codon_start=1
/label=ATP10A
/translation="GADSVVMDLLQPCSSVDARGRHQKKIRSKTQNYLNVYAAEGLRTL
CIAKRVLSKEEYACWLQSHLEAESSLENSEELLFQSAIRLETNLHLLGATGIEDRLQDG
VPETISKLRQAGLQIWVLTGDKQETAVNIAYACKLLDHDEEVITLNATSQEACAALLDQ
CLCYVQSRGLQRAPEKTKGKVSMRFSSLCPPSTSTASGRRPSLVIDGRSLAYALEKNLE
DKFLFLAKQCRSVLCCRSTPLQKSMVVKLVRSKLKAMTLAIGDGANDVSMIQVADVGVG
ISGQEGMQAVMASDFAVPKFRYLERLLILHGHWCYSRLANMVLYFFYKNTMFVGLLFWF
QFFCGFSASTMIDQWYLIFFNLLFSSLPPLVTGVLDRDVPANVLLTNPQLYKSGQNMEE
YRPRTFWFNMADAAFQSLVCFSIPYLAYYDSNVDLFTWGTPIVTIALLTFLLHLGIETK
TWTWLNWITCGFSVLLFFTVALIYNASCATCYPPSNPYWTMQALLGDPVFYLTCLMTPV
AALLPRLFFRSLQGRVFPTQLQLARQLTRKSPRRCSAPKETFAQGRLPKDSGTEHSSGR
TVKTSVPLSQPSWHTQQPVCSLEASGEPSTVDMSMPVREHTLLEGLSAPAPMSSAPGEA
VLRSPGGCPEESKVRAASTGRVTPLSSLFSLPTFSLLNWISSWSLVSRLGSVLQFSRTE
QLADGQAGRGLPVQPHSGRSGLQGPDHRLLIGASSRRSQ"
misc_feature 1977..1982
/label=SDS
misc_feature 2007..2012
/label=SDS
misc_feature 2400..2405
/label=SDS
misc_feature 2551..2556
/label=SDS
misc_feature 3466..3471
/label=SDS
misc_feature 3573..3578
/label=SDS
CDS 3752..3817
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3821..4252
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3965..3970
/label=SDS
repeat_region 4295..4435
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4295..4424
/label=AAV2 ITR
rep_origin 4510..4965
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5247..5351
/gene="bla"
/label=AmpR promoter
CDS 5352..6212
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6383..6971
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggg ccgactcagt
1561 ggtcatggat ctcctgcagc cctgctcttc agttgacgcc agagggaggc atcaaaaaaa
1621 gattcggagc aaaactcaga attacctcaa cgtgtatgcg gcggaaggcc tgcgcacctt
1681 gtgcatcgcc aagagagttc tgagtaaaga agagtatgcc tgctggttgc aaagccacct
1741 agaagccgaa tcctccctgg aaaacagcga ggagctcctc ttccagtctg ccattcgcct
1801 ggagaccaac ctgcacttgt taggtgccac tgggattgaa gaccgcctgc aggacggagt
1861 ccctgaaact atttctaaat tgcgtcaagc gggcctgcag atttgggttc tcactggtga
1921 caaacaagaa acagctgtca acattgcata tgcctgcaaa ctgctggacc acgacgagga
1981 ggtcatcacc ctgaatgcca cctcccagga ggcgtgtgca gccctgctag accagtgcct
2041 atgctacgtg cagtccagag gcctccagag agcccctgag aagaccaagg gcaaagtgag
2101 catgaggttc tcctctctct gcccaccctc cacgtccact gcctctggcc gcagacccag
2161 cctcgtgatc gatgggagaa gcctggccta cgctctcgag aaaaacctgg aggacaaatt
2221 cctcttcctt gccaagcagt gccgctccgt cctctgctgt cggtcgacgc ctctgcagaa
2281 gagcatggtg gtgaagctgg tgcggagcaa gctcaaggcc atgaccctgg ccataggtga
2341 tggagccaat gatgtcagca tgatccaggt ggcagatgtg ggtgtgggaa tctccggcca
2401 ggagggtatg caggcagtga tggccagcga ctttgcagtg ccgaaattcc gatacctgga
2461 gaggctcttg attcttcacg ggcattggtg ctactcccga cttgccaaca tggtgctgta
2521 cttcttctac aaaaacacaa tgttcgtggg cctcctgttt tggttccagt ttttctgtgg
2581 cttctctgca tctaccatga ttgaccagtg gtatctaatc ttctttaatc tgctcttctc
2641 gtcacttccc ccgctcgtga ctggggtgct ggacagggat gtgccagcca atgtgctgct
2701 gaccaacccg cagctctaca agagtggcca gaacatggag gaataccggc cacgaacgtt
2761 ctggtttaac atggccgacg ccgccttcca gagcctggtt tgcttttcca ttccttacct
2821 ggcctactat gactcgaacg tggacctgtt tacctggggg acccctattg tgacaatcgc
2881 gctgctcact ttcctgctcc acctgggcat tgaaaccaaa acctggacct ggctcaactg
2941 gataacgtgt ggcttcagtg tccttttgtt tttcaccgtg gctttgattt acaatgcgtc
3001 ttgtgccacg tgctatcctc cgtccaaccc ttactggact atgcaagcct tactgggtga
3061 cccagtgttt tacttgactt gcctgatgac gcctgtcgct gcactgctgc ccagattgtt
3121 tttcagatcc ctccagggga gggttttccc cacacaactt cagctggcac gtcagttgac
3181 caggaagtcc cccaggagat gcagtgctcc caaagagacc tttgctcagg gacgcctccc
3241 gaaggactcg ggaaccgagc actcatcagg gaggacagtc aagacctctg tgcccctgtc
3301 ccagccttct tggcacacac agcagccggt ctgctccctg gaggccagcg gggagcccag
3361 cacagtggac atgagcatgc cagtgaggga gcacaccctg ctggaggggc tgagcgcacc
3421 ggcccccatg tcctctgcgc caggggaggc tgtcctgagg agtccaggag ggtgtcctga
3481 ggagtccaag gtgagagctg ccagcaccgg cagggtgacc cccctgtctt ccctcttcag
3541 cctgcctacc ttcagcttac tcaactggat ttcctcctgg tcgctggtca gcaggctggg
3601 gagtgtctta cagttctccc ggacggagca gcttgcagat ggacaagcgg gacgtggact
3661 tcctgtccag ccccactcag gccgatcagg acttcaaggg ccagaccaca gactacttat
3721 aggagcatct tcaaggcggt cacagtgtac agactacaaa gaccatgacg gtgattataa
3781 agatcatgac atcgactaca aggatgacga tgacaagtaa ctcgagataa tcaacctctg
3841 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
3901 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
3961 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
4021 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt
4081 tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg
4141 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
4201 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg
4261 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
4321 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
4381 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
4441 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
4501 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
4561 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
4741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
4801 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
4861 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
4921 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
4981 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
5041 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
5101 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
5161 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
5221 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
5281 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
5341 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
5401 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
5461 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
5521 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
5581 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
5641 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
5701 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
5761 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
5821 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
5881 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
5941 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
6001 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
6061 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
6121 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
6181 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
6241 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
6301 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
6361 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
6421 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
6481 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
6541 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
6601 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6661 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6721 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
6781 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
6841 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
6901 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
6961 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
7021 ttgctcacat gt
//
