| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ASTN2-HA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001472 |
| 产品用途: | 3' AAVLINK plasmid for ASTN2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ASTN2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ASTN2-HA载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ASTN2-HA质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ASTN2-HA载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ASTN2-HA质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ASTN2-HA质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ASTN2-HA 7157 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7157)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7157
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3840
/codon_start=1
/label=ASTN2
/translation="GSKPLKRFSGLLSAWAKASGFLSAKALWFRLWSCPSTPWPAARPM
CSSRKTLQMSGKKRCCPHTLKPSMTCCLPSGQFVTALGTMGAALATSSVCLTGRWIPRD
VCALRS*NP*RMALAATTTPKALTALMALMAAVSSCACSRRCPCPTMPLRAPSSCSAVA
WRSTNWLLMENPA*CSQMSARAPSASNLTPNSMIPSLERCYMVTTTGPSM*TKAKSSR*
PLGRTTSSRTFPSWPMGCW*SRCRWRSSAGGSSPSPFRTSNCSLEISGMMRPWVTPWCS
SGGSGATSTV*SSAPSPSQQASLMFSRS*PRRAVGRSCCPSSSTMAPTTSQRPSMAQSS
PASSTFPARRSSSSCGSSIRKRPQSWAARRSSSPCPSSPTSQVC*QPRCCQMTSSFQVW
RFAVRRRGAVHLPVTFAAGQARSS*APHQCCWKSTVWCHFIPSSKTMAQRRPSRVH**V
PTGAQGKGM*SMTGAGVTSAPLMPMGSPTAAPFCSRCCGCPQQWSPPVLWSPWSGWMFS
QLLGPRSPTIFCSIRKWMNTQTLTCTQENS*VLLMTYSLAWAHLV*QLVEAMERSLKSV
STQSSSSVWSPTVSTSSLCMLWIHEGGTQS*AR*P*GRPVHW*MTTRQKK*LTRSTICT
MGTQVERSSRWPTTH*WRSQPRCCSESSTTTTLTMKSLATSSGEVRMSWGPGRPT*FYG
DWRG*VATAPASCGVPTSRAAWKQCPIFSAAARRSGLQAWCGIASSRTPKSRVRRRWCQ
WPETRTGSPRA"
misc_feature 2891..2896
/label=SDS
misc_feature 3660..3665
/label=SDS
misc_feature 3725..3730
/label=SDS
CDS 3856..3882
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3886..3912
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3916..3942
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
terminator 3946..4377
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4090..4095
/label=SDS
repeat_region 4420..4560
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4420..4549
/label=AAV2 ITR
rep_origin 4635..5090
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5372..5476
/gene="bla"
/label=AmpR promoter
CDS 5477..6337
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6508..7096
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggga gcaagcccct
1561 gaaaagattc tcaggtctac tttcagcttg ggccaaggcc tctggcttcc tgtcagcaaa
1621 agctttgtgg ttccgcctgt ggagctgtcc atcaaccccc tggccagctg caagaccgat
1681 gtgctcgtca cggaagaccc tgcagatgtc agggaagaag cgatgctgtc cacatacttt
1741 gaaaccatca atgacctgct gtcttccttc gggccagttc gtgactgctc tcggaacaat
1801 gggggctgca ctcgcaactt caagtgtgtg tctgaccggc aggtggattc ctcgggatgt
1861 gtgtgccctg aggagctgaa acccatgaag gatggctctg gctgctacga ccactccaaa
1921 ggcattgact gctctgatgg ctttaatggc ggctgtgagc agctgtgcct gcagcagacg
1981 ctgcccctgc cctacgatgc cacttcgagc accatcttca tgttctgcgg ttgcgtggag
2041 gagtacaaac tggctcctga tggaaaatcc tgcttaatgc tctcagatgt ctgcgagggc
2101 cccaagtgcc tcaaacctga ctccaaattc aatgataccc tctttggaga gatgctacat
2161 ggttacaaca accggaccca gcatgtgaac caaggccaag tcttccagat gacctttagg
2221 gagaacaact tcatcaagga ctttccccag ctggccgatg ggctgttggt gatcccgctg
2281 ccggtggagg agcagtgccg gggggtcctc tccgagcccc ttccggacct ccaactgctc
2341 actggagata tcaggtatga tgaggccatg ggttacccca tggtgcagca gtggcgggtc
2401 cggagcaacc tctaccgtgt gaagctcagc accatcaccc tcgcagcagg cttcactaat
2461 gttctcaaga tcctgaccaa ggagagcagt cgggaggagc tgctgtcctt catccagcac
2521 tatggctccc actacatcgc agaggccctc tatggctcag agctcacctg catcatccac
2581 tttcccagca agaaggtcca gcagcagctg tggctccagt atcagaaaga gaccacagag
2641 ctgggcagca agaaggagct caagtccatg cccttcatca cctacctctc aggtttgctg
2701 acagcccaga tgctgtcaga tgaccagctc atttcaggtg tggagattcg ctgtgaggag
2761 aaggggcgct gtccatctac ctgtcacctt tgccgccggc caggcaagga gcagctgagc
2821 cccacaccag tgctgctgga aatcaaccgt gtggtgccac tttataccct catccaagac
2881 aatggcacaa aggaggcctt caagagtgca ctgatgagtt cctactggtg ctcagggaaa
2941 ggggatgtga tcgatgactg gtgcaggtgt gacctcagcg cctttgatgc caatgggctc
3001 cccaactgca gcccccttct gcagccggtg ctgcggctgt ccccaacagt ggagccctcc
3061 agtactgtgg tctccttgga gtgggtggat gttcagccag ctattgggac caaggtctcc
3121 gactatattc tgcagcataa gaaagtggat gaatacacag acactgacct gtacacagga
3181 gaattcctga gttttgctga tgacttactc tctggcctgg gcacatcttg tgtagcagct
3241 ggtcgaagcc atggagaggt ccctgaagtc agtatctact cagtcatctt caagtgtctg
3301 gagcccgacg gtctctacaa gttcactctg tatgctgtgg atacacgagg gaggcactca
3361 gagctaagca cggtgaccct gaggacggcc tgtccactgg tagatgacaa caaggcagaa
3421 gaaatagctg acaagatcta caatctgtac aatgggtaca caagtggaaa ggagcagcag
3481 atggcctaca acacactgat ggaggtctca gcctcgatgc tgttccgagt ccagcaccac
3541 tacaactctc actatgaaaa gtttggcgac ttcgtctgga gaagtgagga tgagctgggg
3601 cccaggaagg cccacctgat tctacggcga ctggagaggg tgagtagcca ctgctccagc
3661 ctcctgcgga gtgcctacat ccagagccgc gtggaaacag tgccctatct tttctgccgc
3721 agcgaggagg tccggcctgc aggcatggtg tggtatagca tcctcaagga caccaaaatc
3781 acgtgtgagg agaagatggt gtcaatggcc cgaaacacgt acggggagtc caagggccgg
3841 ggaggtggat ctggttaccc gtatgatgtt ccggattacg ctggctatcc ctacgacgtg
3901 cccgactatg ccgggtaccc ctatgacgtc ccagactacg cataactcga gataatcaac
3961 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
4021 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
4081 tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg
4141 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
4201 ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt tatttgtgaa
4261 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
4321 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaaga
4381 tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
4441 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
4501 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
4561 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
4621 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
4681 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
4741 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
4801 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
4861 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
4921 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
4981 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
5041 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
5101 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
5161 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
5221 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
5281 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
5341 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
5401 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
5461 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
5521 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
5581 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
5641 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
5701 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
5761 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
5821 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
5881 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
5941 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
6001 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
6061 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
6121 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
6181 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
6241 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
6301 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
6361 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
6421 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
6481 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
6541 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
6601 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
6661 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
6721 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
6781 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
6841 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
6901 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
6961 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
7021 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
7081 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
7141 gccttttgct cacatgt
//
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