| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-COL2A1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001657 |
| 产品用途: | 3' AAVLINK plasmid for COL2A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL2A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-COL2A1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-COL2A1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-COL2A1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-COL2A1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-COL2A1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-COL2A1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-COL2A1-FLAG 6135 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6135)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6135
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..2848
/codon_start=1
/label=COL2A1
/translation="VNLDEREAPVLMAPLAEMALLESRVIVVRLVLWELLEPLGPLAPL
APLVQLASKETEEKLVHKAPWDPQDQLEPGESRVLKAPEVTKERLESLAREA*RDTVAS
LVCRVCPALLVLLETKVLLVLLVLLALEVLLAPSVPLAKMVLMESLAPLGLLVPVDDQA
KPALLVLLEILDPLVLQVPLALASTCPPLLA*ARERRAPTPCSTCGPTRQPVA*DSMTP
RWMPHSSPSTTRLRASAAPRAPARTLLAPAET*NSATLSGRVETTGLTPTKAAPWTP*R
FSATWRLARLASTPIQQTFPRRTGGAARARRRNTSGLEKPSMVASISAMEMTIWLPTLP
TSR*PSYACCPRKAPRTSPTTARTALPIWTKQLATSRRPCSSRAPMTWRSGQRAIAGSR
TLP*RMAARNIPVSGARLLSSTGHRRPHASPSLTLHPWT*EGPSRNSVWT*GRSAS"
misc_feature 1817..1822
/label=SDS
misc_feature 1880..1885
/label=SDS
misc_feature 1943..1948
/label=SDS
misc_feature 1988..1993
/label=SDS
misc_feature 2797..2802
/label=SDS
CDS 2855..2920
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2924..3355
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3068..3073
/label=SDS
repeat_region 3398..3538
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3398..3527
/label=AAV2 ITR
rep_origin 3613..4068
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4350..4454
/gene="bla"
/label=AmpR promoter
CDS 4455..5315
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5486..6074
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgaacctg gacgagaggg
1501 aagccccggt gctgatggcc cccctggcag agatggcgct gctggagtca agggtgatcg
1561 tggtgagact ggtgctgtgg gagctcctgg agcccctggg ccccctggct cccctggccc
1621 cgctggtcca actggcaagc aaggagacag aggagaagct ggtgcacaag gccccatggg
1681 accctcagga ccagctggag cccggggaat ccagggtcct caaggcccca gaggtgacaa
1741 aggagaggct ggagagcctg gcgagagagg cctgaaggga caccgtggct tcactggtct
1801 gcagggtctg cccggccctc ctggtccttc tggagaccaa ggtgcttctg gtcctgctgg
1861 tccttctggc cctagaggtc ctcctggccc cgtcggtccc tctggcaaag atggtgctaa
1921 tggaatccct ggccccattg ggcctcctgg tccccgtgga cgatcaggcg aaaccggccc
1981 tgctggtcct cctggaaatc ctggaccccc tggtcctcca ggtccccctg gccctggcat
2041 cgacatgtcc gcctttgctg gcttaggccc gagagagaag ggccccgacc ccctgcagta
2101 catgcgggcc gaccaggcag ccggtggcct gagacagcat gacgccgagg tggatgccac
2161 actcaagtcc ctcaacaacc agattgagag catccgcagc cccgagggct cccgcaagaa
2221 ccctgctcgc acctgcagag acctgaaact ctgccaccct gagtggaaga gtggagacta
2281 ctggattgac cccaaccaag gctgcacctt ggacgccatg aaggttttct gcaacatgga
2341 gactggcgag acttgcgtct accccaatcc agcaaacgtt cccaagaaga actggtggag
2401 cagcaagagc aaggagaaga aacacatctg gtttggagaa accatcaatg gtggcttcca
2461 tttcagctat ggagatgaca atctggctcc caacactgcc aacgtccaga tgaccttcct
2521 acgcctgctg tccacggaag gctcccagaa catcacctac cactgcaaga acagcattgc
2581 ctatctggac gaagcagctg gcaacctcaa gaaggccctg ctcatccagg gctccaatga
2641 cgtggagatc cgggcagagg gcaatagcag gttcacgtac actgccctga aggatggctg
2701 cacgaaacat accggtaagt ggggcaagac tgttatcgag taccggtcac agaagacctc
2761 acgcctcccc atcattgaca ttgcacccat ggacatagga gggcccgagc aggaattcgg
2821 tgtggacata gggccggtct gcttcttgtg tacagactac aaagaccatg acggtgatta
2881 taaagatcat gacatcgact acaaggatga cgatgacaag taactcgaga taatcaacct
2941 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
3001 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
3061 attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc
3121 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt
3181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat
3241 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
3301 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagatc
3361 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
3421 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
3481 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
3541 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
3601 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
3661 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
3721 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
3781 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
3841 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
3901 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
3961 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
4021 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
4081 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
4141 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
4201 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
4261 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
4321 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
4381 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
4441 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
4501 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
4561 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
4621 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
4681 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
4741 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
4801 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
4861 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
4921 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
4981 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
5041 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
5101 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
5161 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
5221 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
5281 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
5341 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
5401 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
5461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
5521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
5581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
5641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
5701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
5761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
5821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
5881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
5941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
6001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
6061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
6121 cttttgctca catgt
//
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