| 载体名称: | LentiGuide-EGFP |
|---|---|
| 载体又名: | LentiGuide-EGFP载体，LentiGuide-EGFP质粒，LentiGuide-EGFP载体质粒 |
| 英文名称: | LentiGuide-EGFP, LentiGuide-EGFP Vector, LentiGuide-EGFP Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体，慢病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8.8 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: |
Blasticidin
|
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | |
| 产品目录号: | 2175596 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
LentiGuide-EGFP载体质粒基本信息
LentiGuide-EGFP质粒图谱载体图谱和LentiGuide-EGFP载体序列质粒序列多克隆位点信息
LentiGuide-EGFP质粒载体简介
LentiGuide-EGFP质粒序列载体序列
LOCUS LentiGuide-EGFP 8889 bp DNA circular SYN 11-JUN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8889)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Jun 11, 2025 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8889
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..241
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 266..341
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 403..614
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 633..642
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 639..1358
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 1374..1962
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(1845..1856)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 2034..2267
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 2299..2523
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2569..2997
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3011..3340
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3191..3326
/label=SV40 ori
/note="SV40 origin of replication"
promoter 3388..3435
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 3454..3828
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 3958..4079
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4128..4144)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4152..4168
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4176..4206)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4221..4242
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4530..5118)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5289..6149)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6150..6254)
/gene="bla"
/label=AmpR promoter
enhancer 6520..6899
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 6901..7099
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 7117..7297
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 7344..7469
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7962..8195
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8380..8424
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8722..8839
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg gagacggacg tctctgtttt agagctagaa atagcaagtt aaaataaggc
301 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgaa ttcgctagct
361 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
421 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
481 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
541 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
601 ccgccagaac acaggaccgg ttctagagcg ctgccaccat ggtgagcaag ggcgaggagc
661 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
721 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
781 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
841 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
901 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
961 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
1021 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
1081 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
1141 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
1201 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
1261 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
1321 ccgggatcac tctcggcatg gacgagctgt acaagtaaac gcgttaagtc gacaatcaac
1381 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
1441 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
1501 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
1561 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
1621 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
1681 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
1741 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
1801 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
1861 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
1921 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
1981 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
2041 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
2101 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
2161 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
2221 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
2281 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
2341 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
2401 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
2461 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
2521 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
2581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
2641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
2701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
2761 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
2821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
2881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
2941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
3001 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
3061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
3121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
3181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
3241 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
3301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
3361 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
3421 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
3481 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
3541 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
3601 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
3661 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
3721 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
3781 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
3841 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
3901 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
3961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
4021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
4081 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
4141 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
4201 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
4261 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
4321 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
4381 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
4441 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
4501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
4561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
4621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
4681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
4741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
4801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
4861 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
4921 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
4981 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
5041 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
5101 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
5161 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
5221 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
5281 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
5341 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
5401 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
5461 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
5521 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
5581 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
5641 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
5701 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
5761 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
5821 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
5881 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
5941 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
6001 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
6061 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
6121 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
6181 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
6241 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
6301 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
6361 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
6421 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
6481 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
6541 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
6601 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
6661 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
6721 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
6781 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
6841 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
6901 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
6961 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
7021 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
7081 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
7141 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
7201 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
7261 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
7321 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
7381 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
7441 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
7501 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
7561 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
7621 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
7681 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
7741 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
7801 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
7861 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
7921 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
7981 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
8041 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
8101 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
8161 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
8221 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
8281 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
8341 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
8401 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
8461 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
8521 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
8581 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
8641 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
8701 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
8761 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
8821 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
8881 taaggtacc
//
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LentiGuide-EGFP质粒载体应用举例
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