| 质粒名称: | pX330-U6-Chimeric_BB-CBh-hSpCas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 42230 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pUC ori vector |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 哺乳细胞表达，CRISPR |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | humanized S. pyogenes Cas9 |
| 插入大小: | 4272 bp |
| 基因又名: | SpCas9；hSpCas9 |
| 启动子 | CBh |
| 标签/融合蛋白: | 3xFLAG (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | agggatggttggttggtggg |
| 3' 测序引物 | CCAATCCTCCCCCTTGCTGT |
|
文献引用方法 |
|
| 材料和方法部分: | pX330-U6-Chimeric_BB-CBh-hSpCas9 was a gift from Feng Zhang (爱迪基因 plasmid # 42230) |
| 参考文献部分: | Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3. 10.1126/science.1231143 PubMed 23287718 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pX330-U6-Chimeric_BB-CBh-hSpCas9 8484 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION A human codon-optimized SpCas9 and chimeric guide RNA expression
plasmid..
ACCESSION .
VERSION .
KEYWORDS pX330-U6-Chimeric_BB-CBh-hSpCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8484)
AUTHORS Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,
Jiang W, Marraffini LA, Zhang F
TITLE Multiplex Genome Engineering Using CRISPR/Cas Systems.
JOURNAL Science. 2013 Jan 3.
PUBMED 23287718
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pX330-U6-Chimeric_BB-CBh-hSpCas9|
FEATURES Location/Qualifiers
source 1..8484
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..241
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 268..343
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 440..725
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 727..983
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
intron 984..1211
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1223..1232
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1232..1297
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1304..1324
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1349..5449
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5450..5497
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
primer_bind complement(5525..5542)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5531..5738
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 5747..5887
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5747..5876
/label=AAV2 ITR
rep_origin 5962..6417
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6049..6068)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6259..6280
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6434..6453)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6553..6575
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6613..6631)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6699..6803
/gene="bla"
/label=AmpR promoter
CDS 6804..7664
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7022..7041)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7835..8423
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8324..8343
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
841 ggggggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
901 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
961 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
1021 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
1081 gcgggcggga cggcccttct cctccgggct gtaattagct gagcaagagg taagggttta
1141 agggatggtt ggttggtggg gtattaatgt ttaattacct ggagcacctg cctgaaatca
1201 ctttttttca ggttggaccg gtgccaccat ggactataag gaccacgacg gagactacaa
1261 ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa
1321 ggtcggtatc cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg
1381 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
1441 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
1501 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
1561 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
1621 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
1681 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
1741 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
1801 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
1861 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
1921 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
1981 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
2041 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
2101 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
2161 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
2221 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
2281 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
2341 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
2401 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
2461 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
2521 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
2581 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
2641 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
2701 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
2761 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
2821 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
2881 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
2941 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
3001 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
3061 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
3121 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
3181 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
3241 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
3301 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
3361 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
3421 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
3481 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
3541 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
3601 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
3661 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
3721 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
3781 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
3841 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
3901 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
3961 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
4021 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
4081 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
4141 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
4201 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
4261 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
4321 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
4381 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
4441 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
4501 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
4561 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
4621 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
4681 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
4741 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
4801 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
4861 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
4921 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
4981 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
5041 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
5101 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
5161 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
5221 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
5281 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
5341 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
5401 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca aaaggccggc
5461 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaagtaa gaattcctag agctcgctga
5521 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
5581 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
5641 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
5701 ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccgcagga acccctagtg
5761 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
5821 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
5881 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
5941 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
6001 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
6061 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
6121 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
6181 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
6241 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
6301 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
6361 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
6421 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
6481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
6541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
6601 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
6661 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
6721 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
6781 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
6841 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
6901 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
6961 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
7021 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
7081 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
7141 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
7201 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
7261 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
7321 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
7381 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
7441 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
7501 ctggagccgg tgagcgtgga agccgcggta tcattgcagc actggggcca gatggtaagc
7561 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
7621 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
7681 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
7741 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
7801 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
7861 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
7921 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
7981 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
8041 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
8101 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
8161 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
8221 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
8281 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
8341 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
8401 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
8461 tttgctggcc ttttgctcac atgt
//
