| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-TRPM1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001504 |
| 产品用途: | 3' AAVLINK plasmid for TRPM1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TRPM1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-TRPM1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-TRPM1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-TRPM1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-TRPM1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-TRPM1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-TRPM1-FLAG 7174 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7174)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7174
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..265
/label=pCALM1
CDS 277..1305
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3887
/codon_start=1
/label=TRPM1
/translation="GDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFN
YVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLV
AISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMI
DMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYA
MEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEV
KSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDR
GLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEIN
ERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLL
RQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVP
ECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETI
SPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMK
SRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQ
AEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLH
GHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC"
misc_feature 2160..2165
/label=SDS
misc_feature 2188..2193
/label=SDS
misc_feature 2587..2592
/label=SDS
CDS 3894..3959
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3963..4394
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4107..4112
/label=SDS
repeat_region 4437..4577
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4437..4566
/label=AAV2 ITR
rep_origin 4652..5107
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5389..5493
/gene="bla"
/label=AmpR promoter
CDS 5494..6354
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6525..7113
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggggatgag gagaacgagc
1501 acaaaaaaca gagaagtatt cccatcggaa caaagatctg tgaattctat aacgcgccca
1561 ttgtcaagtt ctggttttac acaatatcat acttgggcta cctgctgctg tttaactacg
1621 tcatcctggt gcggatggat ggctggccgt ccctccagga gtggatcgtc atctcctaca
1681 tcgtgagcct ggcgttagag aagatacgag agatcctcat gtcagaacca ggcaaactca
1741 gccagaaaat caaagtttgg cttcaggagt actggaacat cacagatctc gtggccattt
1801 ccacattcat gattggagca attcttcgcc tacagaacca gccctacatg ggctatggcc
1861 gggtgatcta ctgtgtggat atcatcttct ggtacatccg tgtcctggac atctttggtg
1921 tcaacaagta tctggggcca tacgtgatga tgattggaaa gatgatgatc gacatgctgt
1981 actttgtggt catcatgctg gtcgtgctca tgagtttcgg agtagcccgt caagccattc
2041 tgcatccaga ggagaagccc tcttggaaac tggcccgaaa catcttctac atgccctact
2101 ggatgatcta tggagaggtg tttgcagacc agatagacct ctacgccatg gaaattaatc
2161 ctccttgtgg tgagaaccta tatgatgagg agggcaagcg gcttcctccc tgtatccccg
2221 gcgcctggct cactccagca ctcatggcgt gctatctact ggtcgccaac atcctgctgg
2281 tgaacctgct gattgctgtg ttcaacaata ccttctttga agtaaaatca atatccaacc
2341 aggtgtggaa gttccagcga tatcagctga ttatgacatt tcatgacagg ccagtcctgc
2401 ccccaccgat gatcatttta agccacatct acatcatcat tatgcgtctc agcggccgct
2461 gcaggaaaaa gagagaaggg gaccaagagg aacgggatcg tggattgaag ctcttcctta
2521 gcgacgagga gctaaagagg ctgcatgagt tcgaggagca gtgcgtgcag gagcacttcc
2581 gggagaagga ggatgagcag cagtcgtcca gcgacgagcg catccgggtc acttctgaaa
2641 gagttgaaaa tatgtcaatg aggttggaag aaatcaatga aagagaaact tttatgaaaa
2701 cttccctgca gactgttgac cttcgacttg ctcagctaga agaattatct aacagaatgg
2761 tgaatgctct tgaaaatctt gcgggaatcg acaggtctga cctgatccag gcacggtccc
2821 gggcttcttc tgaatgtgag gcaacgtatc ttctccggca aagcagcatc aatagcgctg
2881 atggctacag cttgtatcga tatcatttta acggagaaga gttattattt gaggatacat
2941 ctctctccac gtcaccaggg acaggagtca ggaaaaaaac ctgttccttc cgtataaagg
3001 aagagaagga cgtgaaaacg cacctagtcc cagaatgtca gaacagtctt cacctttcac
3061 tgggcacaag cacatcagca accccagatg gcagtcacct tgcagtagat gacttaaaga
3121 acgctgaaga gtcaaaatta ggtccagata ttgggatttc aaaggaagat gatgaaagac
3181 agacagactc taaaaaagaa gaaactattt ccccaagttt aaataaaaca gatgtgatac
3241 atggacagga caaatcagat gttcaaaaca ctcagctaac agtggaaacg acaaatatag
3301 aaggcactat ttcctatccc ctggaagaaa ccaaaattac acgctatttc cccgatgaaa
3361 cgatcaatgc ttgtaaaaca atgaagtcca gaagcttcgt ctattcccgg ggaagaaagc
3421 tggtcggtgg ggttaaccag gatgtagagt acagttcaat cacggaccag caattgacga
3481 cggaatggca atgccaagtt caaaagatca cgcgctctca tagcacagat attccttaca
3541 ttgtgtcgga agctgcagtg caagctgagc ataaagagca gtttgcagat atgcaagatg
3601 aacaccatgt cgctgaagca attcctcgaa tccctcgctt gtccctaacc attactgaca
3661 gaaatgggat ggaaaactta ctgtctgtga agccagatca aactttggga ttcccatctc
3721 tcaggtcaaa aagtttacat ggacatccta ggaatgtgaa atccattcag ggaaagttag
3781 acagatctgg acatgccagt agtgtaagca gcttagtaat tgtgtctgga atgacagcag
3841 aagaaaaaaa ggttaagaaa gagaaagctt ccacagaaac tgaatgctgt acagactaca
3901 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
3961 aactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
4021 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
4081 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
4141 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
4201 tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
4261 ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
4321 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
4381 gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga
4441 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
4501 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
4561 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
4621 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
4681 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
4741 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
4801 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4861 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4921 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4981 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
5041 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
5101 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
5161 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
5221 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
5281 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
5341 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
5401 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
5461 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
5521 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
5581 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
5641 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
5701 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
5761 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
5821 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
5881 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
5941 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
6001 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
6061 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
6121 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
6181 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
6241 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
6301 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
6361 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
6421 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
6481 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
6541 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
6601 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
6661 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
6721 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
6781 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
6841 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
6901 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
6961 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
7021 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
7081 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
7141 ttcctggcct tttgctggcc ttttgctcac atgt
//
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