| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001507 |
| 产品用途: | 3' AAVLINK plasmid for TOP2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TOP2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-TOP2B-FLAG 6769 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6769)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6769
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3482
/codon_start=1
/label=TOP2B
/translation="VLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAA
GLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLG
AESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEED
ETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSP
SDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGR
RIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINK
GPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDS
LLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLD
KDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFS
PSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTT
TPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEP
PSLPRTGRARKEVKYFAESDEEEDDVDFAMFN"
CDS 3489..3554
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3558..3989
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3702..3707
/label=SDS
repeat_region 4032..4172
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4032..4161
/label=AAV2 ITR
rep_origin 4247..4702
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4984..5088
/gene="bla"
/label=AmpR promoter
CDS 5089..5949
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6120..6708
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggttttagaa cctatgctaa
1501 atggaacaga taaaacacca gcattaattt ctgattataa agaatatcat actgacacaa
1561 ctgtgaaatt tgtggtgaaa atgactgaag agaaactagc acaagcagaa gctgctggac
1621 tgcataaagt ttttaaactt caaactactc ttacttgtaa ttccatggta ctttttgatc
1681 atatgggatg tctgaagaaa tatgaaactg tgcaagacat tctgaaagaa ttctttgatt
1741 tacgattaag ttattacggt ttacgtaagg agtggcttgt gggaatgttg ggagcagaat
1801 ctacaaagct gaacaatcaa gcccgtttca ttttagagaa gatacaaggg aaaattacta
1861 tagagaatag gtcaaagaaa gatttgattc aaatgttagt ccagagaggt tatgaatctg
1921 acccagtgaa agcctggaaa gaagcacaag aaaaggcagc agaagaggat gaaacacaaa
1981 accagcatga tgatagttcc tccgattcag gaactccttc aggcccagat tttaattata
2041 ttttaaatat gtctctgtgg tctcttacta aagaaaaagt tgaagaactg attaaacaga
2101 gagatgcaaa agggcgagag gtcaatgatc ttaaaagaaa atctccttca gatctttgga
2161 aagaggattt agcggcattt gttgaagaac tggataaagt ggaatctcaa gaacgagaag
2221 atgttctggc tggaatgtct ggaaaagcaa ttaaaggtaa agttggcaaa cctaaggtga
2281 agaaactcca gttggaagag acaatgccct caccttatgg cagaagaata attcctgaaa
2341 ttacagctat gaaggcagat gccagcaaaa agttgctgaa gaagaagaag ggtgatcttg
2401 atactgcagc agtaaaagtg gaatttgatg aagaattcag tggagcacca gtagaaggtg
2461 caggagaaga ggcattgact ccatcagttc ctataaataa aggtcccaaa cctaagaggg
2521 agaagaagga gcctggtacc agagtgagaa aaacacctac atcatctggt aaacctagtg
2581 caaagaaagt gaagaaacgg aatccttggt cagatgatga atccaagtca gaaagtgatt
2641 tggaagaaac agaacctgtg gttattccaa gagattcttt gcttaggaga gcagcagccg
2701 aaagacctaa atacacattt gatttctcag aagaagagga tgatgatgct gatgatgatg
2761 atgatgacaa taatgattta gaggaattga aagttaaagc atctcccata acaaatgatg
2821 gggaagatga atttgttcct tcagatgggt tagataaaga tgaatataca ttttcaccag
2881 gcaaatcaaa agccactcca gaaaaatctt tgcatgacaa aaaaagtcag gattttggaa
2941 atctcttctc atttccttca tattctcaga agtcagaaga tgattcagct aaatttgaca
3001 gtaatgaaga agattctgct tctgtttttt caccatcatt tggtctgaaa cagacagata
3061 aagttccaag taaaacggta gctgctaaaa agggaaaacc gtcttcagat acagtcccta
3121 agcccaagag agccccaaaa cagaagaaag tagtagaggc tgtaaactct gactcggatt
3181 cagaatttgg cattccaaag aagactacaa caccaaaagg taaaggccga ggggcaaaga
3241 aaaggaaagc atctggctct gaaaatgaag gcgattataa ccctggcagg aaaacatcca
3301 aaacaacaag caagaaaccg aagaagacat cttttgatca ggattcagat gtggacatct
3361 tcccctcaga cttccctact gagccacctt ctctgccacg aaccggtcgg gctaggaaag
3421 aagtaaaata ttttgcagag tctgatgaag aagaagatga tgttgatttt gcaatgttta
3481 attgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
3541 atgacgatga caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt
3601 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
3661 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
3721 gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
3781 tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc
3841 tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
3901 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
3961 ttcaggggga gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac
4021 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
4081 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
4141 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
4201 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
4261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
4321 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
4381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
4441 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
4501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
4561 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
4621 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
4681 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
4741 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
4801 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
4861 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
4921 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
4981 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
5041 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
5101 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
5161 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
5221 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
5281 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
5341 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
5401 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
5461 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
5521 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
5581 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
5641 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
5701 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
5761 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
5821 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
5881 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
5941 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
6001 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
6061 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
6121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
6181 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
6241 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
6301 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
6361 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
6421 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
6481 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
6541 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
6601 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
6661 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
6721 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
