| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-SETD5-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001444 |
| 产品用途: | 5' AAVLINK plasmid for SETD5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETD5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-SETD5-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-SETD5-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-SETD5-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-SETD5-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-SETD5-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-SETD5-FLAG 6732 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6732)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6732
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3433
/codon_start=1
/label=SETD5
/translation="G*LKHHLYPKRLELSTYTKAMRIVALLVSAKTMQTC*AH*RNGSL
AI*WSRMSPSYFGLCLQSHHPLPIQAQRVPSWPHLAHLTQEKRSVEMDTASCFHQSHLL
LLLVAATLLYSLSFVTEKTWIWQK*DTLTPTLTAVLIDLPYSTQVILTWLLIPPSDPLL
RLVSQAEVEMDIRPS*ETQTRHFGQSST*CMPTPL*MLCLEQMDCIEDLL*WGIGSLYI
WMGDIVPLQKDFPADMNMA**KTSLVDPCHLVVKGPVKESHLPPRTHHRGKKYPCWSTE
NGNKKLRKILLVGEVTLHRAKASLQELGKAAVTPFPTLVPMVCRDPQPELHLPLTKNSP
HLIPLCPIWRR*AHQIPEALLHLTADLKRISAVGGWFPHQ*NDSEKEGASPRSSEAA*G
WPKRESPLPHGRVTSQRKTQTLQMEKAQRH*AQHSLKEQQFTALPDTATSSCSVIVLGQ
NHKASFSRVPPPSEDILHSLQDTVIELLH*DLETPPLTVLQNHPSLPRPIPAPPTLCPQ
TRWPHLRGHQGILAASHILETALAAIFQGGAALLVLLALPCRDPQTRQPQIQFLSPAQE
L*VPPPFLRTLGRHCHQTYGLSVCPVLGSQLSTRPPGYLRFPIHSTTHTVGVGVCTSTD
SSHCKGQESRLRRDF"
misc_feature 2638..2643
/label=SDS
misc_feature 2866..2871
/label=SDS
misc_feature 3116..3121
/label=SDS
misc_feature 3222..3227
/label=SDS
CDS 3440..3505
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3521..3952
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3665..3670
/label=SDS
repeat_region 3995..4135
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3995..4124
/label=AAV2 ITR
rep_origin 4210..4665
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4947..5051
/gene="bla"
/label=AmpR promoter
CDS 5052..5912
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6083..6671
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggatgactc aaacatcatc
1501 tgtaccccaa gagactagaa ctcagcacct ataccaaagc aatgagaata gtagctcttc
1561 tagtatctgc aaagacaatg cagacttgtt gagcccatta aagaaatgga agtctcgcta
1621 tctgatggag cagaatgtca ccaagttact tcggcctctg tctccagtca caccaccccc
1681 tcccaattca ggctcaaaga gtccccagct ggccacacct ggctcatctc acccaggaga
1741 agaggagtgt cgaaatggat acagcctcat gttttcacca gtcacatctc ttactactgc
1801 tagtcgctgc aacactcctc tacagtttga gctttgtcac cgaaaagacc tggatttggc
1861 aaaagtagga taccttgact ccaacactaa cagctgtgct gatagacctt ccctactcaa
1921 ctcaggtcat tctgacctgg ctcctcatcc ctccctcgga cccacttctg agactggttt
1981 cccaagcaga agtggagatg gacatcagac cctcgtgaga aactcagacc aggcatttcg
2041 gacagagttc aacttgatgt atgcctactc ccctttgaat gctatgcctc gagcagatgg
2101 actgtatcga ggatctcctc tagtggggga taggaagcct ttacatttgg atgggggata
2161 ttgttcccct gcagaaggat tttccagcag atatgaacat ggcttaatga aagacctctc
2221 tcgtggatcc ttgtcacctg gtggtgaaag ggcctgtgaa ggagtcccat ctgcccccca
2281 gaacccacca cagaggaaaa aagtatccct gctggagtac cgaaaacgga aacaagaagc
2341 taaggaaaat tctgctggtg ggggaggtga ctctgcacag agcaaaagca agtctgcagg
2401 agctgggcaa ggcagcagta actccgtttc cgacactggt gcccatggtg tgcagggatc
2461 ctcagcccga actccatctt cccctcacaa aaaattctcc ccatctcatt cctctatgtc
2521 ccatttggag gcggtaagcc catcagattc cagaggcact tcttcatctc actgcagacc
2581 tcaagagaat atcagcagta ggtggatggt tcccacatca gtagaacgac tccgagaagg
2641 agggagcatc cccaaggtcc tccgaagcag cgtgagggtg gcccaaaagg gagagccctc
2701 tcccacatgg gagagtaaca tcacagagaa agactcagac cctgcagatg gagaaggccc
2761 agagacatta agctcagcac tctctaaagg agcaacagtt tacagccctt ccagatacag
2821 ctaccagctc ctgcagtgtg atagtcctcg gacagaatca caaagcctcc ttcagcagag
2881 ttcctccccc ttcagaggac atcctacaca gtctccagga tacagttatc gaactactgc
2941 actgagacct ggaaaccccc cctctcacgg ttcttcagaa tcatccctct cttccacgtc
3001 ctattccagc cccgcccacc ctgtgtccac agactcgttg gccccattta cggggacacc
3061 agggtatttt agcagccagc cacattctgg aaacagcact ggcagcaatc ttccaaggag
3121 gagctgccct tctagtgctg ctagccctac cctgcaggga ccctcagact cgccaacctc
3181 agattcagtt tctcagtcca gcacaggaac tctgagttcc acctcctttc ctcagaactc
3241 taggtcgtca ttgccatcag acttacggac tatcagtctg cccagtgctg ggcagtcagc
3301 tgtctaccag gcctccaggg tatctgcggt ttccaattca cagcactacc cacaccgtgg
3361 gagtgggggt gtgcaccagt accgactcca gccactgcaa gggtcaggag tcaagactca
3421 gacgggactt tcctgtacag actacaaaga ccatgacggt gattataaag atcatgacat
3481 cgactacaag gatgacgatg acaagtaaga attctgatga ctcgagataa tcaacctctg
3541 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
3601 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
3661 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
3721 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt
3781 tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg
3841 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
3901 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg
3961 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
4021 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
4081 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
4141 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
4201 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
4261 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
4321 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
4381 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
4441 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
4501 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
4561 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
4621 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
4681 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
4741 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
4801 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
4861 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
4921 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
4981 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
5041 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
5101 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
5161 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
5221 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
5281 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
5341 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
5401 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
5461 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
5521 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
5581 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
5641 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
5701 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
5761 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
5821 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
5881 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
5941 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
6001 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
6061 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
6121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
6181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
6241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
6301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
6481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
6541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
6601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
6661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
6721 ttgctcacat gt
//
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