| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001414 |
| 产品用途: | 3' AAVLINK plasmid for RAI1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
RAI1
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG 6922 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6922)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6922
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3635
/codon_start=1
/label=RAI1
/translation="VSSSPQKEGRVSQRARVPKPGAGSKLSDRPLHALKRKSAFMAPVP
TKKRNLVLRSRSSSSSNASGNGGDGKEERPEGSPTLFKRMSSPKKAKPTKGNGEPATKL
PPPETPDACLKLASRAAFQGAMKTKVLPPRKGRGLKLEAIVQKITSPSLKKFACKAPGA
SPGNPLSPSLSDKDRGLKGAGGSPVGVEEGLVNVGTGQKLPTSGADPLCRNPTNRSLKG
KLMNSKKLSSTDCFKTEAFTSPEALQPGGTALAPKKRSRKGRAGAHGLSKGPLEKRPYL
GPALLLTPRDRASGTQGASEDNSGGGGKKPKMEELGLASQPPEGRPCQPQTRAQKQPGH
TNYSSYSKRKRLTRGRAKNTTSSPCKGRAKRRRQQQVLPLDPAEPEIRLKYISSCKRLR
SDSRTPAFSPFVRVEKRDAFTTICTVVNSPGDAPKPHRKPSSSASSSSSSSSFSLDAAG
ASLATLPGGSILQPRPSLPLSSTMHLGPVVSKALSTSCLVCCLCQNPANFKDLGDLCGP
YYPEHCLPKKKPKLKEKVRPEGTCEEASLPLERTLKGPECAAAATAGKPPRPDGPADPA
KQGPLRTSARGLSRRLQSCYCCDGREDGGEEAAPADKGRKHECSKEAPAEPGGEAQEHW
VHEACAVWTGGVYLVAGKLFGLQEAMKVAVDMMCSSCQEAGATIGCCHKGCLHTYHYPC
ASDAGCIFIEENFSLKCPKHKRLP"
misc_feature 1501..1506
/label=SDS
misc_feature 1693..1698
/label=SDS
misc_feature 2399..2404
/label=SDS
misc_feature 2800..2805
/label=SDS
misc_feature 2809..2814
/label=SDS
misc_feature 2902..2907
/label=SDS
misc_feature 3106..3111
/label=SDS
misc_feature 3298..3303
/label=SDS
misc_feature 3343..3348
/label=SDS
misc_feature 3454..3459
/label=SDS
CDS 3642..3707
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3711..4142
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3855..3860
/label=SDS
repeat_region 4185..4325
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4185..4314
/label=AAV2 ITR
rep_origin 4400..4855
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5137..5241
/gene="bla"
/label=AmpR promoter
CDS 5242..6102
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6273..6861
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtctccagc agcccccaga
1501 aggagggcag ggtgagccag cgggcaaggg tccccaaacc tggtgcaggc agcaagctct
1561 ctgaccggcc cctccatgcg ctcaaaagga agtcggcctt catggcgccg gtccccacca
1621 agaagcggaa cctggtcttg cggagccgca gcagcagcag cagcaacgcc agtggcaatg
1681 ggggagatgg gaaggaggag aggcctgagg gttcccccac cctcttcaag aggatgtctt
1741 ctcccaagaa agccaagccc accaagggca atggcgagcc tgccacaaag ctcccacccc
1801 cggagacccc cgatgcctgc ctcaagctcg cctctcgggc agccttccag ggggccatga
1861 agaccaaggt gctgccaccc cggaagggcc ggggcctgaa gctggaagcc atcgtgcaga
1921 agatcacctc gcccagcctc aagaagttcg catgtaaagc gccaggggcc tctcctggta
1981 atcctctgag cccatccctt tccgacaaag accgtgggct caagggtgct gggggcagcc
2041 cagtgggggt ggaagaaggc ctggtaaatg tgggcaccgg gcagaagctc ccaacttctg
2101 gggctgatcc gttatgcaga aatccaacca acagatcctt aaaaggcaaa ctcatgaaca
2161 gtaagaaact gtcttctact gactgtttca aaaccgaggc cttcacatcc ccggaggccc
2221 tgcagcctgg ggggactgcc ctggcgccta agaagaggag ccggaaaggc cgggcagggg
2281 cccatggact ctccaaaggc ccgctggaga agcggcccta tcttggcccg gctctgctcc
2341 tgactccccg agacagggcc agtggcacac aaggggccag tgaggacaac tctggtggag
2401 gaggcaagaa gccaaagatg gaggagctgg gcctggcctc ccagcccccg gagggcaggc
2461 cctgccagcc ccagacaagg gcacagaaac agccaggcca caccaactac agcagctatt
2521 ccaagcggaa gcgcctcact cggggccggg ccaagaacac cacctcttca ccctgtaagg
2581 ggcgtgccaa gcgacgacga cagcagcagg tgctgcccct ggatcccgca gagcctgaaa
2641 tccgcctcaa gtacatttcc tcttgcaagc ggctgaggtc agacagccgg acccccgcct
2701 tctcaccctt cgtgcgggtg gagaagcgag acgcgttcac caccatatgc actgttgtca
2761 actcccctgg agatgcgccc aagccccaca ggaagccttc ctcctctgcc tcctcttcct
2821 catcctcgtc ctcgttctcc ttggatgcag ccggggcctc cctggccaca ctccctggag
2881 gctccatcct gcagccgcgg ccctccttgc ccctctcctc cacgatgcac ttggggcctg
2941 tggtttccaa ggccctgagt acctcttgcc ttgtttgctg cctctgccaa aacccggcca
3001 acttcaagga ccttggggac ctctgtgggc cctactaccc tgaacactgc ctccccaaaa
3061 agaagccaaa actcaaggag aaggtgcggc cagaaggcac ctgtgaggag gcctcgctgc
3121 cgcttgagag aacactcaaa ggtcccgagt gtgcagctgc cgccactgcc gggaagcccc
3181 ccaggcctga cggcccagct gacccggcca agcagggccc actgcgcacc agtgcccggg
3241 gcctgtcccg gaggctgcag agctgctact gctgtgatgg ccgggaggat gggggcgagg
3301 aggcagcccc agccgacaag ggtcgcaaac atgagtgcag caaggaggct ccggcagagc
3361 ccggcgggga ggcccaggag cactgggtgc atgaggcctg tgccgtgtgg accggcggcg
3421 tctacctggt ggccgggaag ctctttgggc tgcaggaggc catgaaggtg gccgtggaca
3481 tgatgtgttc cagctgccaa gaagccgggg ccaccatcgg gtgctgccac aaaggatgcc
3541 tccacaccta ccactacccg tgtgccagcg atgcaggttg catattcatc gaagagaact
3601 tttctttgaa atgtcccaaa cataagaggc tgccgtgtac agactacaaa gaccatgacg
3661 gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa ctcgagataa
3721 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
3781 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
3841 ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat
3901 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
3961 ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt
4021 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
4081 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
4141 aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
4201 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
4261 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
4321 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
4381 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
4441 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
4501 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
4561 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
4621 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
4681 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
4741 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
4801 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
4861 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
4921 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
4981 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
5041 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
5101 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
5161 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
5221 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
5281 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
5341 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
5401 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
5461 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
5521 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
5581 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
5641 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
5701 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
5761 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
5821 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
5881 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
5941 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
6001 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
6061 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
6121 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
6181 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
6241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
6301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
6361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
6421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
6481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
6541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
6601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
6661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
6721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
6781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
6841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
6901 tgctggcctt ttgctcacat gt
//
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