| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-PREX1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001522 |
| 产品用途: | 3' AAVLINK plasmid for PREX1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PREX1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-PREX1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-PREX1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-PREX1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-PREX1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-PREX1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-PREX1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-PREX1-FLAG 6319 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6319)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6319
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3020
/codon_start=1
/label=PREX1
/translation="VCFKVAEEDQEDSGHDTMSYRDSYSECNSNRDSVLSYTSVRSNSS
YLGSDEMGSGDELPCDMRIPSDKQDKLHGCLEHLFNQVDSINALLKGPVMSRAFEETKH
FPMNHSLQEFKQKEECTIRGRSLIQISIQEDPWNLPNSIKTLVDNIQRYVEDGKNQLLL
ALLKCTDTELQLRRDAIFCQALVAAVCTFSEQLLAALGYRYNNNGEYEESSRDASRKWL
EQVAATGVLLHCQSLLSPATVKEERTMLEDIWVTLSELDNVTFSFKQLDENYVANTNVF
YHIEGSRQALKVIFYLDSYHFSKLPSRLEGGASLRLHTALFTKVLENVEGLPSPGSQAA
EDLQQDINAQSLEKVQQYYRKLRAFYLERSNLPTDASTTAVKIDQLIRPINALDELCRL
MKSFVHPKPGAAGSVGAGLIPISSELCYRLGACQMVMCGTGMQRSTLSVSLEQAAILAR
SHGLLPKCIMQATDIMRKQGPRVEILAKNLRVKDQMPQGAPRLYRLCQPPVDGDL"
misc_feature 1501..1506
/label=SDS
misc_feature 1510..1515
/label=SDS
misc_feature 1879..1884
/label=SDS
misc_feature 2212..2217
/label=SDS
CDS 3027..3092
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3108..3539
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3252..3257
/label=SDS
repeat_region 3582..3722
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3582..3711
/label=AAV2 ITR
rep_origin 3797..4252
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4534..4638
/gene="bla"
/label=AmpR promoter
CDS 4639..5499
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5670..6258
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtgcttc aaggtggccg
1501 aggaggacca ggaggactca ggccacgaca ccatgagtta tcgcgactcc tacagcgagt
1561 gtaacagcaa tcgagactcg gtcctgtcct acaccagcgt gagaagtaac agctcctact
1621 tgggcagcga cgagatgggg tctggagatg agctgccctg tgacatgcgg atcccatctg
1681 acaagcagga caagcttcat ggctgcctgg agcacctctt taaccaggtg gactccatca
1741 atgctctcct caaggggcca gtcatgagcc gggctttcga agagaccaag catttcccta
1801 tgaaccacag cttacaagag tttaaacaga aagaagagtg tacaatccgt ggccggagcc
1861 tgatccagat tagcatccag gaggacccct ggaacctccc caactccatc aagaccctgg
1921 tggacaacat tcagagatat gtggaagatg ggaagaacca gctgctcctg gccttgctga
1981 agtgcacaga cacggagctg cagctgcgca gagacgcgat cttctgccag gccctggtgg
2041 ccgccgtgtg caccttctcc gagcagctgc tggcggccct gggctaccgc tacaacaaca
2101 atggcgagta cgaggagagc agccgcgacg ccagccgcaa gtggctggag caggtggcgg
2161 ccacgggcgt cctgctgcac tgccagtccc tgctctcgcc agccacagtg aaggaggaac
2221 ggaccatgct ggaggacatc tgggtgacgc tgtcagagct ggacaatgtc accttctcct
2281 ttaagcagct ggacgagaac tatgtggcca acaccaacgt cttctaccac attgagggca
2341 gccggcaggc gctgaaggtc atcttctacc tcgacagcta ccacttctcc aagctgccct
2401 cccgcctgga gggtggggcc agcctgaggc tgcacacagc gctgttcacg aaagtgctgg
2461 agaacgtgga ggggctgcct tctccaggca gccaggccgc ggaggatttg cagcaggaca
2521 tcaacgcgca gtccctggag aaagttcagc agtattaccg caaactcagg gcattttacc
2581 tggagcggtc taacctgccc acggatgcca gcaccacggc ggtaaagata gaccagctga
2641 tccgccccat caatgccctg gatgagctct gccgcctcat gaagtccttt gtccacccaa
2701 agcctggtgc tgctgggagt gtgggcgccg gcctcatccc catctcctcg gagctctgct
2761 accgcctggg ggcctgccag atggtcatgt gtggcacagg catgcagagg agcaccctga
2821 gcgtgtccct ggagcaggcg gccatcttgg cacggagcca cgggttgctg cccaagtgca
2881 tcatgcaggc cacggacatc atgcggaagc agggcccaag ggtggagatt ctggccaaaa
2941 acctgcgagt caaggaccag atgccccagg gtgctccgcg cctctaccgc ctctgccagc
3001 cgccggtgga tggggacctc tgtacagact acaaagacca tgacggtgat tataaagatc
3061 atgacatcga ctacaaggat gacgatgaca agtaagaatt ctgatgactc gagataatca
3121 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
3181 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
3241 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
3301 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
3361 gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg
3421 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
3481 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
3541 gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga
3601 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
3661 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
3721 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
3781 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
3841 acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc
3901 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
3961 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
4021 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
4081 acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc
4141 tattcttttg atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg
4201 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
4261 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
4321 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
4381 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
4441 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
4501 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
4561 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
4621 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
4681 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
4741 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
4801 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
4861 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
4921 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
4981 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
5041 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
5101 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
5161 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
5221 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
5281 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
5341 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
5401 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
5461 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
5521 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
5581 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
5641 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
5701 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
5761 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
5821 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
5881 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
5941 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
6001 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
6061 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
6121 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
6181 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
6241 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
6301 tggccttttg ctcacatgt
//
