| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-PCDH11X-HA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001474 |
| 产品用途: | 3' AAVLINK plasmid for PCDH11X expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PCDH11X |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-PCDH11X-HA载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-PCDH11X-HA质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-PCDH11X-HA载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-PCDH11X-HA质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-PCDH11X-HA质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-PCDH11X-HA 6748 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6748)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6748
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3431
/codon_start=1
/label=PCDH11X
/translation="VIAVDNDTGMNAEVRYSIVGGNTRDLFAIDQETGNITLMEKCDVT
DLGLHRVLVKANDLGQPDSLFSVVIVNLFVNESVTNATLINELVRKSTEAPVTPNTEIA
DVSSPTSDYVKILVAAVAGTITVVVVIFITAVVRCRQAPHLKAAQKNKQNSEWATPNPE
NRQMIMMKKKKKKKKHSPKNLLLNFVTIEETKADDVDSDGNRVTLDLPIDLEEQTMGKY
NWVTTPTTFKPDSPDLARHYKSASPQPAFQIQPETPLNSKHHIIQELPLDNTFVACDSI
SKCSSSSSDPYSVSDCGYPVTTFEVPVSVHTRPPMKEVVRSCTPMKESTTMEIWIHPQP
QRKSEGKVAGKSQRRVTFHLPEGSQESSSDGGLGDHDAGSLTSTSHGLPLGYPQEEYFD
RATPSNRTEGDGNSDPESTFIPGLKKAAEITVQPTVEEASDNCTQECLIYGHSDACWMP
ASLDHSSSSQAQASALCHSPPLSQASTQHHSPRVTQTIALCHSPPVTQTIALCHSPPPI
QVSALHHSPPLVQATALHHSPPSAQASALCYSPPLAQAAAISHSSPLPQVIALHRSQAQ
SSVSLQQGWVQGADGLCSVDQGVQGSATSQFYTMSERLHPSDDSIKVIPLTTFTPRQQA
RPSRGDSPIMEEHPL"
misc_feature 1538..1543
/label=SDS
misc_feature 2431..2436
/label=SDS
misc_feature 2662..2667
/label=SDS
misc_feature 3057..3062
/label=SDS
misc_feature 3129..3134
/label=SDS
CDS 3447..3473
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3477..3503
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3507..3533
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
terminator 3537..3968
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3681..3686
/label=SDS
repeat_region 4011..4151
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4011..4140
/label=AAV2 ITR
rep_origin 4226..4681
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4963..5067
/gene="bla"
/label=AmpR promoter
CDS 5068..5928
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6099..6687
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtaattgct gttgacaatg
1501 acactggcat gaatgcagag gttcgttaca gcattgtagg aggaaacaca agagatctgt
1561 ttgcaatcga ccaagaaaca ggcaacataa cattgatgga gaaatgtgat gttacagacc
1621 ttggtttaca cagagtgttg gtcaaagcta atgacttagg acagcctgat tctctcttca
1681 gtgttgtaat tgtcaatctg ttcgtgaatg agtcggtgac caatgctaca ctgattaatg
1741 aactggtgcg caaaagcact gaagcaccag tgaccccaaa tactgagata gctgatgtat
1801 cctcaccaac tagtgactat gtcaagatcc tggttgcagc tgttgctggc accataactg
1861 tcgttgtagt tattttcatc actgctgtag taagatgtcg ccaggcacca caccttaagg
1921 ctgctcagaa aaacaagcag aattctgaat gggctacccc aaacccagaa aacaggcaga
1981 tgataatgat gaagaaaaag aaaaagaaga agaagcattc ccctaagaac ttgctgctta
2041 attttgtcac tattgaagaa actaaggcag atgatgttga cagtgatgga aacagagtca
2101 cactagacct tcctattgat ctagaagagc aaacaatggg aaagtacaat tgggtaacta
2161 cacctactac tttcaagccc gacagccctg atttggcccg acactacaaa tctgcctctc
2221 cacagcctgc cttccaaatt cagcctgaaa ctcccctgaa ttcgaagcac cacatcatcc
2281 aagaactgcc tctcgataac acctttgtgg cctgtgactc tatctccaag tgttcctcaa
2341 gcagttcaga tccctacagc gtttctgact gtggctatcc agtgacgacc ttcgaggtac
2401 ctgtgtccgt acacaccaga ccgccaatga aggaggttgt gcgatcttgc acccccatga
2461 aagagtctac aactatggag atctggattc atccccaacc acagcggaaa tctgaaggga
2521 aagtggcagg aaagtcccag cggcgtgtca catttcacct gccagaaggc tctcaggaaa
2581 gcagcagtga tggtggactg ggagaccatg atgcaggcag ccttaccagc acatctcatg
2641 gcctgcccct tggctatcct caggaggagt actttgatcg tgctacaccc agcaatcgca
2701 ctgaagggga tggcaactcc gatcctgaat ctactttcat acctggacta aagaaagctg
2761 cagaaataac tgttcaacca actgtggaag aggcctctga caactgcact caagaatgtc
2821 tcatctatgg ccattctgat gcctgctgga tgccggcatc tctggatcat tccagctctt
2881 cgcaagcaca ggcctctgct ctatgccaca gcccaccact gtcacaggcc tctactcagc
2941 accacagccc acgagtgaca cagaccattg ctctctgcca cagccctcca gtgacacaga
3001 ccatcgcatt gtgccacagc ccaccaccga tacaggtgtc tgctctccac cacagtcctc
3061 ctctagtgca ggctactgca cttcaccaca gcccaccatc agcacaggcc tcagccctct
3121 gctacagccc tcctttagca caggctgctg caatcagcca cagctctcct ctgccacagg
3181 ttattgccct ccatcgtagt caggcccaat catcagtcag tttgcagcaa ggttgggtgc
3241 aaggtgctga tgggctatgc tctgttgatc agggagtgca aggtagtgca acatctcagt
3301 tttacaccat gtctgaaaga cttcatccca gtgatgattc aattaaagtc attcctttga
3361 caaccttcac tccacgccaa caggccagac cgtccagagg tgattccccc attatggaag
3421 aacatccctt gggaggtgga tctggttacc cgtatgatgt tccggattac gctggctatc
3481 cctacgacgt gcccgactat gccgggtacc cctatgacgt cccagactac gcataactcg
3541 agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
3601 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
3661 ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga
3721 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
3781 ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct
3841 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
3901 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
3961 ttttttaaag atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
4021 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
4081 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
4141 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
4201 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
4261 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
4321 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
4381 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
4441 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
4501 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
4561 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
4621 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
4681 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
4741 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
4801 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
4861 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
4921 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
4981 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
5041 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
5101 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
5161 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
5221 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
5281 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
5341 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
5401 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
5461 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
5521 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
5581 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
5641 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
5701 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
5761 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
5821 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
5881 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
5941 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
6001 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
6061 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
6121 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
6181 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
6241 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
6301 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
6361 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
6421 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
6481 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
6541 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
6601 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
6661 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
6721 gccttttgct ggccttttgc tcacatgt
//
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