| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-MED13L-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001435 |
| 产品用途: | 3' AAVLINK plasmid for MED13L expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MED13L |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-MED13L-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-MED13L-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-MED13L-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-MED13L-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-MED13L-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-MED13L-FLAG 7237 bp DNA circular SYN 25-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7237)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7237
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..265
/label=pCALM1
CDS 277..1305
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3938
/codon_start=1
/label=MED13L
/translation="DFLTISPFSLPFWERLLLDPYGGHRDVAYIVVCPENEALLEGAKT
FFRDLSAVYEMCRLGQHKPICKVLRDGIMRVGKTVAQKLTDELVSEWFNQPWSGEENDN
HSRLKLYAQVCRHHLAPYLATLQLDSSLLIPPKYQTPPAAAQGQATPGNAGPLAPNGSA
APPAGSAFNPTSNSSSTNPAASSSASGSSVPPVSSSASAPGISQISTTSSSGFSGSVGG
QNPSTGGISADRTQGNIGCGGDTDPGQSSSQPSQDGQESVTERERIGIPTEPDSADSHA
HPPAVVIYMVDPFTYAAEEDSTSGNFWLLSLMRCYTEMLDNLPEHMRNSFILQIVPCQY
MLQTMKDEQVFYIQYLKSMAFSVYCQCRRPLPTQIHIKSLTGFGPAASIEMTLKNPERP
SPIQLYSPPFILAPIKDKQTELGETFGEASQKYNVLFVGYCLSHDQRWLLASCTDLHGE
LLETCVVNIALPNRSRRSKVSARKIGLQKLWEWCIGIVQMTSLPWRVVIGRLGRLGHGE
LKDWSILLGECSLQTISKKLKDVCRMCGISAADSPSILSACLVAMEPQGSFVVMPDAVT
MGSVFGRSTALNMQSSQLNTPQDASCTHILVFPTSSTIQVAPANYPNEDGFSPNNDDMF
VDLPFPDDMDNDIGILMTGNLHSSPNSSPVPSPGSPSGIGVGSHFQHSRSQGERLLSRE
APEELKQQPLALGYFVSTAKAENLPQWFWSSCPQAQNQCPLFLKASLHHHISVAQTDEL
LPARNSQRVPHPLDSKTTSDVLRFVLEQYNALSWLTCNPATQDRTSCLPVHFVVLTQLY
NAIMNIL"
misc_feature 2377..2382
/label=SDS
misc_feature 3050..3055
/label=SDS
CDS 3945..4010
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4026..4457
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4170..4175
/label=SDS
repeat_region 4500..4640
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4500..4629
/label=AAV2 ITR
rep_origin 4715..5170
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5452..5556
/gene="bla"
/label=AmpR promoter
CDS 5557..6417
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6588..7176
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggatttcctc accatctcgc
1501 cattctcctt gccgttttgg gagaggctct tgttggaccc atatgggggc caccgtgatg
1561 ttgcctatat tgtggtgtgt ccagaaaatg aggccttgct cgaaggagcc aaaactttct
1621 tcagggactt gagtgctgta tacgagatgt gtaggcttgg gcagcacaag cccatctgca
1681 aagtgctacg tgacgggatc atgcgcgtgg gaaaaactgt ggcacagaag ctgacagatg
1741 agcttgtgag tgagtggttt aaccagcctt ggagcggcga ggagaatgac aatcattcca
1801 gactcaaact ttatgcgcaa gtttgccgcc atcacctagc accttattta gccactctgc
1861 agcttgatag cagcctattg ataccaccta aataccagac cccaccagca gcagcacagg
1921 gacaagctac gccagggaat gctgggccct tagctccaaa tggatcagca gctcccccag
1981 ctggcagtgc atttaatccc acctcgaata gtagttctac aaatcctgca gcaagtagtt
2041 ctgcatctgg ttcctctgtg ccaccggtct catcgtctgc ctctgctcct ggtattagcc
2101 agataagcac tacctcttct tcaggattca gtggtagtgt tggagggcag aaccccagca
2161 ctgggggcat ttctgcggat agaacgcaag ggaacatagg ctgtggtgga gacactgacc
2221 ctgggcagag ctcttctcag ccctcacagg atggacaaga gagtgttaca gaaagggaga
2281 gaataggaat tcccacggag cctgactctg cagacagcca tgcccaccct ccagctgttg
2341 tcatttacat ggtggacccg ttcacgtatg ctgcagagga ggactccact tctgggaact
2401 tttggctgtt gagcttgatg cgctgctaca cagaaatgct ggataattta cctgagcata
2461 tgagaaattc tttcattctc cagattgtgc cttgccagta catgctgcag acaatgaagg
2521 atgagcaagt tttctacatt caatacttga agtccatggc attttcagtg tactgtcagt
2581 gcaggcgacc actgcctaca cagatccaca ttaaatccct cacgggattt gggcctgcag
2641 ccagcattga gatgaccctc aagaaccctg agcggcccag cccaatccag ctttactccc
2701 ctccctttat attggcccca atcaaagaca agcagacaga gctgggagag acgtttggtg
2761 aggcgagcca gaaatacaat gtgctcttcg tgggctattg tctgtctcac gaccagcgct
2821 ggcttttggc ttcctgcact gacctccatg gggaattatt agagacctgc gttgtaaata
2881 ttgctttacc aaacaggtca cggaggagta aagtatctgc acgtaaaatt ggactacaga
2941 agttatggga gtggtgcata gggattgtcc aaatgacatc tctaccctgg agagttgtaa
3001 tcgggcgact tgggcgtctt ggccatgggg agcttaaaga ttggagtatc ctccttggag
3061 aatgttcact acagacaatc agcaaaaagc tcaaggatgt gtgccggatg tgtggaatct
3121 ctgccgcaga ctctccttct atccttagtg cctgcctggt tgccatggag ccccaggggt
3181 cctttgtagt gatgccagat gctgtcacaa tgggctctgt ttttggccga agtactgcac
3241 tgaacatgca gtcatctcag ctcaacaccc ctcaagatgc ttcttgtaca cacatcttgg
3301 tgttcccaac atcatcaacc atccaggtgg ctccagccaa ctaccccaat gaagatgggt
3361 ttagccccaa caatgatgat atgtttgttg accttccatt cccagatgat atggacaatg
3421 atattggcat attaatgact gggaacctcc attcctctcc caactcttcc ccagtaccct
3481 ccccaggctc tccttctgga attggtgtgg gctctcactt ccagcatagt cggagccagg
3541 gtgagcgtct tctttctaga gaagcaccag aggagctaaa gcagcagccc ctggcccttg
3601 ggtattttgt atcaactgcc aaagctgaga atcttcccca gtggttttgg tcatcgtgtc
3661 cccaggctca aaaccagtgc cctctcttct taaaggcttc gctgcatcac cacatttcag
3721 tagcacagac agacgaactt ctgcctgcca ggaattctca gcgggttcca caccctcttg
3781 actccaaaac cacgtcggat gttttaaggt ttgttttgga gcagtacaac gctctgtcct
3841 ggctcacgtg caatccggcc acccaggacc gtacttcctg ccttcccgtc cactttgtgg
3901 tgctcactca gttgtacaat gccatcatga atatactttg tacagactac aaagaccatg
3961 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag taagaattct
4021 gatgactcga gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
4081 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
4141 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc
4201 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
4261 cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac
4321 catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
4381 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
4441 tgtgggaggt tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca
4501 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
4561 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
4621 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
4681 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
4741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
4801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
4861 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
4921 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
4981 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
5041 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
5101 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
5161 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
5221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
5281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
5341 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
5401 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
5461 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
5521 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
5581 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
5641 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
5701 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
5761 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
5821 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
5881 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
5941 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
6001 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
6061 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
6121 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
6181 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
6241 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
6301 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
6361 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
6421 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
6481 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
6541 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
6601 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
6661 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
6721 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
6781 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
6841 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
6901 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
6961 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
7021 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
7081 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
7141 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
7201 cggttcctgg ccttttgctg gccttttgct cacatgt
//
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