| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-KIF14-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001516 |
| 产品用途: | 3' AAVLINK plasmid for KIF14 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KIF14 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-KIF14-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KIF14-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KIF14-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KIF14-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KIF14-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KIF14-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KIF14-FLAG 6652 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6652)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6652
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3365
/codon_start=1
/label=KIF14
/translation="EIYVNKKRLEMETLATKQALEDHSIRHARILEALETEKQKIAKEV
QILQQNRNNRDKTFTVQTTWSSMKLSMMIQEANAISSKLKTYYVFGRHDISDKSSSDTS
IRVRNLKLGISTFWSLEKFESKLAAMKELYESNGSNRGEDAFCDPEDEWEPDITDAPVS
SLSRRRSRSLMKNRRISGCLHDIQVHPIKNLHSSHSSGLMDKSSTIYSNSAESFLPGIC
KELIGSSLDFFGQSYDEERTIADSLINSFLKIYNGLFAISKAHEEQDEESQDNLFSSDR
AIQSLTIQTACAFEQLVVLMKHWLSDLLPCTNIARLEDELRQEVKKLGGYLQLFLQGCC
LDISSMIKEAQKNAIQIVQQAVKYVGQLAVLKGSKLHFLENGNNKAASVQEEFMDAVCD
GVGLGMKILLDSGLEKAKELQHELFRQCTKNEVTKEMKTNAMGLIRSLENIFAESKIKS
FRRQVQEENFEYQDFKRMVNRAPEFLKLKHCLEKAIEIIISALKGCHSDINLLQTCVES
IRNLASDFYSDFSVPSTSVGSYESRVTHIVHQELESLAKSLLFCFESEESPDLLKPWET
YNQNTKEEHQQSKSSGIDGSKNKGVPKRVYELHGSSPAVSSEECTPSRIQWV"
misc_feature 1983..1988
/label=SDS
misc_feature 2650..2655
/label=SDS
CDS 3372..3437
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3441..3872
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3585..3590
/label=SDS
repeat_region 3915..4055
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3915..4044
/label=AAV2 ITR
rep_origin 4130..4585
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4867..4971
/gene="bla"
/label=AmpR promoter
CDS 4972..5832
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6003..6591
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggaaatatat gtcaacaaaa
1501 agcgattaga aatggagaca ttggctacaa aacaggcttt agaagaccat agcatccgcc
1561 atgcaagaat tctggaagct ttagaaactg aaaagcaaaa aattgctaaa gaagtacaaa
1621 ttctacagca gaatcggaat aatagggata aaacttttac agtgcagaca acttggagct
1681 ctatgaaact ctcaatgatg attcaggaag ccaatgctat cagcagcaaa ttgaaaacat
1741 actatgtttt tggcagacat gatatatcag ataaaagtag ttctgacact tctattcggg
1801 ttcgtaacct gaaactagga atctcaacat tctggagtct ggaaaagttt gaatctaaac
1861 ttgcagcaat gaaagaactt tatgagagta atggtagtaa caggggtgaa gatgcctttt
1921 gtgatcctga agatgaatgg gaacccgaca ttacagatgc accagtttct tcactttcta
1981 gaaggaggag taggagtttg atgaagaaca gaagaatttc tggttgttta catgacatac
2041 aagtccatcc aattaagaat ttgcattctt cacattcatc aggtttaatg gacaaatcaa
2101 gcactattta ctcaaattca gcagagtcct ttcttcctgg aatttgcaaa gaattgattg
2161 gttcttcgtt agattttttt ggacagagtt atgatgaaga aagaactata gcagacagcc
2221 taattaatag ttttcttaaa atttataatg ggctatttgc catttccaag gctcatgaag
2281 aacaagatga agaaagtcaa gataacttgt tttcttctga tcgagcaatc cagtcactta
2341 ctattcagac tgcatgtgct tttgagcagc tagtagtgct aatgaaacac tggctgagtg
2401 atttactgcc ttgtaccaac atagcaagac ttgaggatga attgagacaa gaagttaaaa
2461 aactgggagg ctacttacag ttatttttgc agggatgctg tttggatatt tcatcaatga
2521 taaaagaggc tcaaaagaat gcaatccaaa ttgtacaaca agctgtaaag tatgtggggc
2581 agttagcagt tctgaaaggg agcaagctac attttctaga aaacggtaac aataaagctg
2641 ccagtgtcca ggaggaattc atggatgctg tttgtgatgg tgtaggctta ggaatgaaga
2701 ttttattaga ttctggactg gaaaaagcaa aagaacttca gcatgaactc tttaggcagt
2761 gtacaaaaaa tgaggttacc aaagaaatga aaactaatgc catgggattg attagatctc
2821 ttgaaaacat ctttgctgaa tcgaaaatta aaagtttcag aaggcaagta caagaagaaa
2881 actttgaata ccaagatttc aagaggatgg ttaatcgtgc tccagaattc ttaaagttaa
2941 aacattgctt agagaaagct attgaaatta ttatttctgc actgaaagga tgccatagtg
3001 atataaatct tctccagact tgtgttgaaa gtattcgcaa cttggccagt gatttttaca
3061 gtgacttcag tgtgccttct acttctgttg gcagctatga gagtagagta actcacattg
3121 tccaccagga actagaatct ctagctaagt ctctcctctt ttgttttgaa tctgaagaaa
3181 gccctgattt gttgaaaccc tgggaaactt ataatcaaaa taccaaagaa gaacaccaac
3241 aatctaaatc aagcgggatt gacggcagta agaataaagg tgtaccaaag cgtgtctatg
3301 agctccatgg ctcatcccca gcagtgagct cagaggaatg cacacccagt aggattcagt
3361 gggtgtgtac agactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca
3421 aggatgacga tgacaagtaa ctcgagataa tcaacctctg gattacaaaa tttgtgaaag
3481 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
3541 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
3601 ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
3661 ggctcggctg ttgggcactg acaattccgt ggtgtttatt tgtgaaattt gtgatgctat
3721 tgctttattt gtaaccatct agctttattt gtgaaatttg tgatgctatt gctttatttg
3781 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
3841 aggttcaggg ggagatgtgg gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg
3901 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
3961 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
4021 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
4081 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
4141 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
4201 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
4261 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
4321 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
4381 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
4441 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
4501 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
4561 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
4621 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
4681 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
4741 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
4801 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
4861 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
4921 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
4981 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
5041 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
5101 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
5161 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
5221 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
5281 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
5341 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
5401 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
5461 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
5521 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
5581 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
5641 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
5701 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
5761 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
5821 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
5881 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
5941 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
6001 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
6061 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
6121 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
6181 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
6241 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
6301 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
6361 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
6421 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
6481 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
6541 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
6601 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
