| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-KDM2B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001351 |
| 产品用途: | 3' AAVLINK plasmid for KDM2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KDM2B-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KDM2B-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KDM2B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KDM2B-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KDM2B-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KDM2B-FLAG 6498 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6498)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6498
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
/note="Splicing donor"
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3211
/codon_start=3
/gene="KDM2B"
/gene_synonym="CXXC2; Fbl10; FBXL10; JHDM1B; PCCX2"
/product="lysine-specific demethylase 2B isoform a"
/EC_number="1.14.11.27"
/label=KDM2B
/note="isoform a is encoded by transcript variant 1;jumonji
C domain-containing histone demethylase 1B; F-box and
leucine-rich repeat protein 10; JEMMA (Jumonji domain,
EMSY-interactor, methyltransferase motif) protein; F-box
protein FBL10; lysine (K)-specific demethylase 2B;
F-box/LRR-repeat protein 10; CXXC-type zinc finger protein
2; protein-containing CXXC domain 2; [Histone-H3]-lysine-36
demethylase 1B; jmjC domain-containing histone
demethylation protein 1B"
/db_xref="GI:54112382"
/db_xref="CCDS:CCDS41850.1"
/db_xref="GeneID:84678"
/db_xref="HGNC:HGNC:13610"
/db_xref="MIM:609078"
/protein_id="NP_115979.3"
/translation="QEPAKRRSECEEAPRRRSDEHSKKVPPDGLLRRKSDDVHLRKKRK
YEKPQELSGRKRASSLQTSPGSSSHLSPRPPLGSSLSPWWRSSLTYFQQQLKPGKEDKL
FRKKRRSWKNAEDRMALANKPLRRFKQEPEDELPEAPPKTRESDHSRSSSPTAGPSTEG
AEGPEEKKKVKMRRKRRLPNKELSRELSKELNHEIQRTENSLANENQQPIKSEPESEGE
EPKRPPGICERPHRFSKGLNGTPRELRHQLGPSLRSPPRVISRPPPSVSPPKCIQMERH
VIRPPPISPPPDSLPLDDGAAHVMHREVWMAVFSYLSHQDLCVCMRVCRTWNRWCCDKR
LWTRIDLNHCKSITPLMLSGIIRRQPVSLDLSWTNISKKQLSWLINRLPGLRDLVLSGC
SWIAVSALCSSSCPLLRTLDVQWVEGLKDAQMRDLLSPPTDNRPGQMDNRSKLRNIVEL
RLAGLDITDASLRLIIRHMPLLSKLHLSYCNHVTDQSINLLTAVGTTTRDSLTEINLSD
CNKVTDQCLSFFKRCGNICHIDLRYCKQVTKEGCEQFIAEMSVSVQFGQVEEKLLQKLS
"
misc_feature 1515..1520
/label=SDS
misc_feature 1683..1688
/label=SDS
CDS 3218..3283
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3287..3718
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3431..3436
/label=SDS
repeat_region 3761..3901
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3761..3890
/label=AAV2 ITR
rep_origin 3976..4431
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4713..4817
/gene="bla"
/label=AmpR promoter
CDS 4818..5678
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5849..6437
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcaggaac ctgccaagcg
1501 gaggagtgag tgtgaggagg cgccccggcg caggtcggat gagcactcga agaaggtgcc
1561 gccggacggc cttctgcgca gaaagtctga cgacgtgcac ctgaggaaga agcggaaata
1621 cgagaagccc caggagctga gtggacgcaa gcgggcctca tcgcttcaaa cgtcccccgg
1681 ttcctcctct cacctctcgc cgaggccccc tctaggcagc agcctcagcc cctggtggag
1741 atccagtctc acttacttcc agcagcagct caaacctggc aaagaagata agcttttcag
1801 gaaaaagcgg cggtcctgga agaacgccga ggaccgcatg gcgctggcca acaagcccct
1861 ccggcgcttc aagcaggaac ccgaggacga actgcccgag gcgcccccca agaccaggga
1921 gagcgaccac tcccgctcca gctcccccac cgcgggaccc agcaccgaag gggccgaggg
1981 cccggaggag aagaagaagg tgaagatgcg ccggaagcgg cggcttccca acaaggagct
2041 gagcagggag ctgagcaagg agctcaacca cgagatccag aggacggaga acagcctggc
2101 caacgagaac cagcagccca tcaagtcgga gcctgagagc gagggcgagg agcccaagcg
2161 gcccccgggc atctgcgagc gtccccaccg cttcagcaag gggctcaacg gcaccccccg
2221 ggagctgcgg caccagctgg ggcccagcct gcgcagcccg ccccgtgtca tctcccggcc
2281 cccaccctcc gtgtccccgc ccaagtgtat ccagatggag cgccatgtga tccggccacc
2341 ccccatcagc cccccgcctg actcgctacc cctggacgat ggggcagccc acgtcatgca
2401 cagggaggtg tggatggccg tcttcagcta cctcagccac caagacctgt gtgtgtgcat
2461 gcgggtctgc aggacctgga accgctggtg ctgcgataag cggttgtgga cccgcattga
2521 cctgaaccac tgcaagtcta tcacacccct gatgctgagt ggcatcatcc ggcgacagcc
2581 cgtctccctc gacctcagct ggaccaatat ctccaagaag cagctgagct ggctcatcaa
2641 ccggctgcct gggctccggg acttggtgct gtcaggctgc tcatggatcg cggtctcggc
2701 cctttgcagc tccagttgtc cgctgctccg gaccctggat gtccagtggg tggagggact
2761 aaaggatgcc cagatgcggg atctcctgtc cccgcccaca gacaacaggc caggtcagat
2821 ggacaatcgg agcaagctcc ggaacatcgt ggagctgcgc ctggcaggcc tggacatcac
2881 agatgcctcc ctgcggctca tcatccgcca catgcccctg ctctccaagc tccacctcag
2941 ttactgtaac cacgtcaccg accagtctat caacctgctc actgctgttg gcaccaccac
3001 ccgagactcc ttaaccgaga tcaacctgtc tgactgcaat aaggtcactg atcagtgcct
3061 gtccttcttc aaacgctgtg gaaacatctg tcatattgac ctgaggtact gcaagcaagt
3121 caccaaggaa ggctgtgagc agttcatagc cgagatgtct gtgagtgtcc agtttgggca
3181 agtagaagaa aaactcctgc aaaaactgag ttgtacagac tacaaagacc atgacggtga
3241 ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa
3301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
3361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
3421 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
3481 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
3541 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga
3601 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
3661 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
3721 atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
3781 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
3841 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
3901 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
3961 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
4021 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
4081 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
4141 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
4201 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
4261 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
4321 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
4381 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
4441 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
4501 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
4561 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
4621 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
4681 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
4741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
4801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
4861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
4921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
4981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
5041 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
5101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
5161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
5221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
5281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
5341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
5401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
5461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
5521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
5581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
5641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
5701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
5761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
5821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
5881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
5941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
6001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
6061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
6121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
6181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
6241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
6301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
6361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
6421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
6481 ggccttttgc tcacatgt
//
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