| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001543 |
| 产品用途: | 3' AAVLINK plasmid for ITSN1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ITSN1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG 6454 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6454)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6454
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3167
/codon_start=1
/label=ITSN1
/translation="VIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLF
PSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIF
QKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQ
LPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQR
VTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHV
QCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSS
GTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINE
RTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKS
NPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLG
RTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP"
misc_feature 1564..1569
/label=SDS
misc_feature 2462..2467
/label=SDS
CDS 3174..3239
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3243..3674
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3387..3392
/label=SDS
repeat_region 3717..3857
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3717..3846
/label=AAV2 ITR
rep_origin 3932..4387
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4669..4773
/gene="bla"
/label=AmpR promoter
CDS 4774..5634
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5805..6393
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgattggg atgtacgact
1501 acaccgcgca gaatgacgat gagctggcct tcaacaaggg ccagatcatc aacgtcctca
1561 acaaggagga ccctgactgg tggaaaggag aagtcaatgg acaagtgggg ctcttcccat
1621 ccaattatgt gaagctgacc acagacatgg acccaagcca gcaatggtgt tcagacttac
1681 atctcttgga tatgttgacc ccaactgaaa gaaagcgaca aggatacatc cacgagctca
1741 ttgtcaccga ggagaactat gtgaatgacc tgcagctggt cacagagatt tttcaaaaac
1801 ccctgatgga gtctgagctg ctgacagaaa aagaggttgc tatgattttt gtgaactgga
1861 aggagctgat tatgtgtaat atcaaactac taaaagcgct gagagtccgc aagaagatgt
1921 ccggggagaa gatgcctgtg aagatgattg gagacatcct gagcgcacag ctgccgcaca
1981 tgcagcccta catccgcttc tgcagccgcc agctcaacgg ggctgccctg atccagcaga
2041 agacggacga ggccccagac ttcaaggagt tcgtcaaaag attggcaatg gatcctcggt
2101 gtaaagggat gccactctct agttttatac tgaagcctat gcaacgggta acaagatacc
2161 cactgatcat taaaaatatc ctggaaaaca cccctgaaaa ccacccggac cacagccact
2221 tgaagcacgc cctggagaag gcggaagagc tctgttccca ggtgaacgaa ggggtgcggg
2281 agaaggagaa ctctgaccgg ctggagtgga tccaggccca cgtgcagtgt gaaggcctgt
2341 ctgagcaact tgtgttcaat tcagtgacca attgcttggg gccgcgcaaa tttctgcaca
2401 gtgggaagct ctacaaggcc aagagcaaca aggagctgta tggcttcctt ttcaacgact
2461 tcctcctgct gactcagatc acgaagcctt tggggtcttc tggcaccgac aaagtcttca
2521 gccccaaatc aaacctgcag tataaaatgt ataaaacacc tattttccta aatgaggttc
2581 tagtaaaatt acccaccgac ccttctggag acgagcccat cttccacatc tcccacattg
2641 accgcgtcta tactctccga gcagaaagca taaatgaaag gactgcctgg gtgcagaaaa
2701 tcaaagctgc ttctgaactc tacatagaga ctgagaaaaa gaagcgcgag aaagcgtacc
2761 tggtccgttc ccaaagggca acaggcattg gaaggttgat ggtgaacgtg gttgaaggca
2821 tcgagttgaa accctgtcgg tcacatggaa agagcaaccc gtactgtgag gtgaccatgg
2881 gttcccagtg ccacatcacc aagacgatcc aggacactct gaaccccaag tggaattcca
2941 actgccagtt cttcatccga gacctggagc aggaagtcct ctgcatcact gtgttcgaga
3001 gggaccagtt ctcaccagat gattttttgg gtcggacgga gatccgcgtg gcggacatca
3061 agaaagacca gggctccaaa ggtccagtta cgaagtgtct tctgctgcac gaagtcccca
3121 cgggagagat tgtggtccgc ttggacctgc agttgtttga tgagccgtgt acagactaca
3181 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
3241 aactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
3301 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
3361 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
3421 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
3481 tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
3541 ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
3601 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
3661 gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga
3721 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
3781 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
3841 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
3901 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
3961 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
4021 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
4081 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4141 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4201 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4261 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
4321 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
4381 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
4441 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
4501 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
4561 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
4621 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
4681 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
4741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
4801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
4861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
4921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
4981 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
5041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
5101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
5161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
5221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
5281 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
5341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
5401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
5461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
5521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
5581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
5641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
5701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
5761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
5821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
5881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
5941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
6001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
6061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
6121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
6181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
6241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
6301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
6361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
6421 ttcctggcct tttgctggcc ttttgctcac atgt
//
