| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-IQGAP3-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001510 |
| 产品用途: | 3' AAVLINK plasmid for IQGAP3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | IQGAP3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-IQGAP3-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-IQGAP3-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-IQGAP3-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-IQGAP3-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-IQGAP3-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-IQGAP3-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-IQGAP3-FLAG 6610 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6610)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6610
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3311
/codon_start=1
/label=IQGAP3
/translation="VEQPQDVVTGNPTVVRLVVRFYRNGRGQSALQEILGKVIQDVLED
KVLSVHTDPVHLYKNWINQTEAQTGQRSHLPYDVTPEQALSHPEVQRRLDIALRNLLAM
TDKFLLAITSSVDQIPYGMRYVAKVLKATLAEKFPDATDSEVYKVVGNLLYYRFLNPAV
VAPDAFDIVAMAAGGALAAPQRHALGAVAQLLQHAAAGKAFSGQSQHLRVLNDYLEETH
LKFRKFIHRACQVPEPEERFAVDEYSDMVAVAKPMVYITVGELVNTHRLLLEHQDCIAP
DHQDPLHELLEDLGELPTIPDLIGESIAADGHTDLSKLEVSLTLTNKFEGLEADADDSN
TRSLLLSTKQLLADIIQFHPGDTLKEILSLSASREQEAAHKQLMSRRQACTAQTPEPLR
RHRSLTAHSLLPLAEKQRRVLRNLRRLEALGLVSARNGYQGLVDELAKDIRNQHRHRHR
RKAELVKLQATLQGLSTKTTFYEEQGDYYSQYIRACLDHLAPDSKSSGKGKKQPSLHYT
AAQLLEKGVLVEIEDLPASHFRNVIFDITPGDEAGKFEVNAKFLGVDMERFQLHYQDLL
QLQYEGVAVMKLFNKAKVNVNLLIFLLNKKFLRK"
misc_feature 1781..1786
/label=SDS
misc_feature 1937..1942
/label=SDS
misc_feature 2705..2710
/label=SDS
misc_feature 3284..3289
/label=SDS
CDS 3318..3383
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3399..3830
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3543..3548
/label=SDS
repeat_region 3873..4013
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3873..4002
/label=AAV2 ITR
rep_origin 4088..4543
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4825..4929
/gene="bla"
/label=AmpR promoter
CDS 4930..5790
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5961..6549
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtggagcag ccccaggacg
1501 tggtgacagg caacccaaca gtggtgaggc tggtggtgag attctaccgt aatgggcggg
1561 gacagagtgc cctgcaggag attctgggca aggttatcca ggatgtgcta gaagacaaag
1621 tgctcagcgt ccacacagac cctgtccacc tctataagaa ctggatcaac cagactgagg
1681 cccagacagg gcagcgcagc catctcccat atgatgtcac cccggagcag gccttgagcc
1741 accccgaggt ccagagacga ctggacatcg ccctacgcaa cctcctcgcc atgactgata
1801 agttcctttt agccatcacc tcatctgtgg accaaattcc gtatgggatg cgatatgtgg
1861 ccaaagtcct gaaggcaact ctggcagaga aattccctga cgccacagac agcgaggtct
1921 ataaggtggt cgggaacctc ctgtactacc gcttcctgaa cccagctgtg gtggctcctg
1981 acgccttcga cattgtggcc atggcagctg gtggagccct ggctgccccc cagcgccatg
2041 ccctgggggc tgtggctcag ctcctacagc acgctgcggc tggcaaggcc ttctctgggc
2101 agagccagca cctacgggtc ctgaatgact atctggagga aacacacctc aagttcagga
2161 agttcatcca tagagcctgc caggtgccag agccagagga gcgttttgca gtggacgagt
2221 actcagacat ggtggctgtg gccaaaccca tggtgtacat caccgtgggg gagctggtca
2281 acacgcacag gctgttgctg gagcaccagg actgcattgc ccctgatcac caagaccccc
2341 tgcatgagct cctggaggat cttggggagc tgcccaccat ccctgacctt attggtgaga
2401 gcatcgctgc agatgggcac acggacctga gcaagctaga agtgtccctg acgctgacca
2461 acaagtttga aggactagag gcagatgctg atgactccaa cacccgtagc ctgcttctga
2521 gcaccaagca gctgttggcc gatatcatac agttccatcc tggggacacc ctcaaggaga
2581 tcctgtccct ctcggcttcc agagagcaag aagcagccca caagcagctg atgagccgac
2641 gccaggcctg tacagcccag acaccggagc cactgcgacg acaccgctca ctgacagctc
2701 actccctcct gccactggca gagaagcagc ggcgcgtcct gcggaaccta cgccgacttg
2761 aagccctggg gttggtcagc gccagaaatg gctaccaggg gctagtggac gagctggcca
2821 aggacatccg caaccagcac agacacaggc acaggcggaa ggcagagctg gtgaagctgc
2881 aggccacatt acagggcctg agcactaaga ccaccttcta tgaggagcag ggtgactact
2941 acagccagta catccgggcc tgcctggacc acctggcccc cgactccaag agttctggga
3001 aggggaagaa gcagccttct cttcattaca ctgctgctca gctcctggaa aagggtgtct
3061 tggtggaaat tgaagatctt cccgcctctc acttcagaaa cgtcatcttt gacatcacgc
3121 cgggagatga ggcaggaaag tttgaagtaa atgccaagtt cctgggtgtg gacatggagc
3181 gatttcagct tcactatcag gatctcctgc agctccagta tgagggtgtg gctgtcatga
3241 aactcttcaa caaggccaaa gtcaatgtca accttctcat cttcctcctc aacaagaagt
3301 ttttgcggaa gtgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg
3361 actacaagga tgacgatgac aagtaagaat tctgatgact cgagataatc aacctctgga
3421 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
3481 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
3541 ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct
3601 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg
3661 tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg
3721 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
3781 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag
3841 accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
3901 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
3961 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
4021 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
4081 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
4141 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
4201 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
4261 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
4321 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
4381 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
4441 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
4501 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
4561 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
4621 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
4681 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
4741 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
4801 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
4861 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
4921 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
4981 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
5041 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
5101 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
5161 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
5221 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
5281 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
5341 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
5401 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
5461 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
5521 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
5581 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
5641 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
5701 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
5761 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
5821 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
5881 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
5941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
6001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
6061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
6121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
6181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
6241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
6301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
6361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
6421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
6481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
6541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
6601 gctcacatgt
//
