| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-ERBIN-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001462 |
| 产品用途: | 3' AAVLINK plasmid for ERBIN expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ERBIN |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ERBIN-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ERBIN-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ERBIN-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ERBIN-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ERBIN-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ERBIN-FLAG 6541 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6541)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6541
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3254
/codon_start=1
/label=ERBIN
/translation="VNGHSEETSQSPNRTEPHDSDCSVDLGISKSTEDLSPQKSGPVGS
VVKSHSITNMEIGGLKIYDILSDNGPQQPSTTVKITSAVDGKNIVRSKSATLLYDQPLQ
VFTGSSSSSDLISGTKAIFKFDSNHNPEEPNIIRGPTSGPQSAPQIYGPPQYNIQYSSS
AAVKDTLWHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYH
LHQRLGPARHGEMWAISPNDRLIPAVTRSTIQRQSSVSSTASVNLGDPGSTRRAQIPEG
DYLSYREFHSAGRTPPMMPGSQRPLSARTYSIDGPNASRPQSARPSINEIPERTMSVSD
FNYSRTSPSKRPNARVGSEHSLLDPPGKSKVPRDWREQVLRHIEAKKLEKKHPQTSSSG
DPCQDGIFISGQQNYSSATLSHKDVPPDSLMKMPLSNGQMGQPLRPQANYSQIHHPPQA
SVARHPSREQLIDYLMLKVAHQPPYTQPHCSPRQGHELAKQEIRVRVEKDPELGFSISG
GVGGRGNPFRPDDDGIFVTRVQPEGPASKLLQPGDKIIQANGYSFINIEHGQAVSLLKT
FQNTVELIIVREVSS"
misc_feature 2034..2039
/label=SDS
CDS complement(2453..2464)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 3261..3326
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3330..3761
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3474..3479
/label=SDS
repeat_region 3804..3944
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3804..3933
/label=AAV2 ITR
rep_origin 4019..4474
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4756..4860
/gene="bla"
/label=AmpR promoter
CDS 4861..5721
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5892..6480
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtaaatgga cattctgagg
1501 aaacttccca gtctcctaat aggactgaac cacatgacag tgattgttct gttgacttag
1561 gtatttccaa aagcactgaa gatctctccc ctcagaaaag tggtccagtt ggatctgttg
1621 tgaaatctca tagcataact aatatggaga ttggagggct aaaaatctat gatattctta
1681 gtgataatgg acctcagcag ccaagtacaa ccgttaaaat cacatctgct gttgatggaa
1741 aaaatatagt caggagcaag tctgccacac tgttgtatga tcaaccattg caggtattta
1801 ctggttcttc ctcatcttct gatttaatat caggaacaaa ggcaattttc aagtttgatt
1861 caaatcataa tcccgaagag ccaaatataa taagaggccc cacaagtggc ccacaatctg
1921 cacctcaaat atatggtcct ccacagtata atatccaata cagtagcagt gctgcagtca
1981 aagacacttt gtggcactcc aaacaaaatc cccaaataga ccatgccagt tttcctcctc
2041 agctccttcc tagatcagag agcacagaaa atcaaagtta tgctaaacat tctgccaata
2101 tgaatttctc taatcataac aatgttcgag ctaatactgc ataccattta catcagagac
2161 ttggcccagc aagacatggg gaaatgtggg ccatctcacc aaacgaccga cttattcctg
2221 cagtaactcg aagtacaatc cagcgacaaa gtagtgtgtc ctccacagcc tctgtaaatc
2281 ttggtgatcc aggctctaca aggcgggctc agattcctga aggagattat ttatcataca
2341 gagagttcca ctcagcggga agaactcctc caatgatgcc aggatcacag agaccccttt
2401 ctgcacgaac atacagcata gatggtccaa atgcatcaag acctcagagt gctcgaccct
2461 ctattaatga aataccagag agaactatgt cagttagtga tttcaattat tcacggacta
2521 gtccttcaaa aagaccaaat gcaagggttg gttctgagca ttctttatta gatcctccag
2581 gaaaaagtaa agttcctcgt gactggagag aacaagtact tcgacatatt gaagccaaaa
2641 agttagaaaa gaagcatccc cagacatcca gttcaggaga tccttgtcaa gatggtatat
2701 tcatttcagg acagcagaac tactcatcag ccacacttag tcacaaagat gttcctccag
2761 acagcttgat gaaaatgcct ttgagtaatg gacagatggg ccagcctctc aggcctcagg
2821 caaattatag tcaaatacat cacccccctc aggcatctgt ggcaaggcat ccctctagag
2881 aacaactaat tgattacttg atgctgaaag tggcccacca gcctccatat acacagcccc
2941 attgttctcc tagacaaggc catgaactgg caaaacaaga gattcgagtg agggttgaaa
3001 aggatccaga acttggattt agcatatcag gtggtgtcgg gggtagagga aacccattca
3061 gacctgatga tgatggtata tttgtaacaa gggtacaacc tgaaggacca gcatcaaaat
3121 tactgcagcc aggtgataaa attattcagg ctaatggcta cagttttata aatattgaac
3181 atggacaagc agtgtccttg ctaaaaactt tccagaatac agttgaactc atcattgtac
3241 gagaagtttc ctcatgtaca gactacaaag accatgacgg tgattataaa gatcatgaca
3301 tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat
3361 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
3421 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
3481 gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
3541 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg
3601 tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg
3661 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
3721 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg
3781 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
3841 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
3901 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
3961 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
4021 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
4081 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
4141 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
4201 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
4261 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
4321 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
4381 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
4441 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
4501 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
4561 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
4621 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
4681 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
4741 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
4801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
4861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
4921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
4981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
5041 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
5101 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
5161 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
5221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
5281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
5341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
5401 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
5461 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
5521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
5581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
5641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
5701 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
5761 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
5821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
5881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
5941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
6001 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
6061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
6121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
6181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
6241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
6301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
6361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
6421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
6481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
6541 t
//
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