| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-DIP2C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001498 |
| 产品用途: | 3' AAVLINK plasmid for DIP2C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DIP2C |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-DIP2C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-DIP2C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-DIP2C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-DIP2C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-DIP2C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-DIP2C-FLAG 6856 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6856)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6856
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3557
/codon_start=1
/label=DIP2C
/translation="AIDSIHQVGVYCLALVPANTLPKTPLGGIHLSETKQLFLEGSLHP
CNVLMCPHTCVTNLPKPRQKQPEIGPASVMVGNLVSGKRIAQASGRDLGQIEDNDQARK
FLFLSEVLQWRAQTTPDHILYTLLNCRGAIANSLTCVQLHKRAEKIAVMLMERGHLQDG
DHVALVYPPGIDLIAAFYGCLYAGCVPITVRPPHPQNIATTLPTVKMIVEVSRSACLMT
TQLICKLLRSREAAAAVDVRTWPLILDTDDLPKKRPAQICKPCNPDTLAYLDFSVSTTG
MLAGVKMSHAATSAFCRSIKLQCELYPSREVAICLDPYCGLGFVLWCLCSVYSGHQSIL
IPPSELETNPALWLLAVSQYKVRDTFCSYSVMELCTKGLGSQTESLKARGLDLSRVRTC
VVVAEERPRIALTQSFSKLFKDLGLHPRAVSTSFGCRVNLAICLQGTSGPDPTTVYVDM
RALRHDRVRLVERGSPHSLPLMESGKILPGVRIIIANPETKGPLGDSHLGEIWVHSAHN
ASGYFTIYGDESLQSDHFNSRLSFGDTQTIWARTGYLGFLRRTELTDANGERHDALYVV
GALDEAMELRGMRYHPIDIETSVIRAHKSVTECAVFTWTNLLVVVVELDGSEQEALDLV
PLVTNVVLEEHYLIVGVVVVVDIGVIPINSRGEKQRMHLRDGFLADQLDPIYVAYNM"
CDS 3564..3629
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3645..4076
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3789..3794
/label=SDS
repeat_region 4119..4259
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4119..4248
/label=AAV2 ITR
rep_origin 4334..4789
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5071..5175
/gene="bla"
/label=AmpR promoter
CDS 5176..6036
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6207..6795
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggcgattgac agtatacatc
1501 aagttggagt ttattgcctg gccttggtgc cagcaaacac cctccccaaa accccgcttg
1561 gtgggatcca tttatcagaa acaaaacagc tttttctgga gggctctctg cacccctgca
1621 atgtcctaat gtgcccccac acctgcgtca caaacttgcc taagcctcga cagaagcagc
1681 cagaaatcgg ccctgcctct gtgatggtgg ggaacctggt ctctgggaag agaatcgccc
1741 aggccagtgg cagagacctg ggtcagatcg aagataacga ccaggcacgc aagttcctgt
1801 tcctctcaga ggtcttgcag tggagagcac agaccacccc ggaccacatc ctctacacgc
1861 tgctcaactg tcggggtgcg atagcgaact cgctgacctg cgtgcagctg cacaagagag
1921 ctgagaagat cgccgtgatg ctgatggaga ggggccacct tcaggacggc gaccacgtgg
1981 ccttggtcta ccccccagga atagacctga tagcagcgtt ttatggttgc ctgtacgcag
2041 gctgtgtgcc aataaccgtc cgtcccccgc acccacagaa catcgcgacg acgttgccta
2101 ccgtcaagat gattgtggag gtgagtcgct ctgcctgtct gatgacgaca cagctgatct
2161 gtaagttgct gcggtccagg gaggcggcgg cggctgtgga cgtcaggacg tggcccctca
2221 tcctggacac agatgatttg ccaaagaagc ggcctgccca gatctgcaaa ccttgcaacc
2281 cagacactct tgcatatctc gacttcagcg tgtccacaac tgggatgcta gctggcgtaa
2341 agatgtctca cgcagccacc agtgccttct gccgttccat taagctgcag tgtgaacttt
2401 acccctctag agaagtggcc atctgcctgg acccttactg tggactggga tttgtcctct
2461 ggtgcctctg cagtgtgtat tctgggcacc agtccatcct gatcccgccc tctgagctgg
2521 aaaccaaccc cgccttgtgg cttcttgccg tgagtcagta caaagtccga gacacgtttt
2581 gctcctactc cgtgatggag ctgtgcacca aggggctggg ctcgcaaaca gagtccctca
2641 aggcgcgagg gctggacttg tcccgagtga ggacctgcgt ggttgtggcg gaagagaggc
2701 ctcggatcgc actcacacag tcgttctcaa agctgtttaa ggacctgggc cttcacccgc
2761 gggccgtcag cacctcgttc ggttgcaggg tgaacctggc gatttgcttg cagggaacct
2821 caggacctga cccaaccact gtctacgtgg acatgagagc cctgagacac gacagagtcc
2881 gcttagtgga aagaggatcc cctcatagtc tgcccctgat ggaatcggga aagatacttc
2941 caggggttcg gattataatt gccaacccag aaacaaaagg accgctgggg gactcacacc
3001 ttggagagat ttgggttcac agtgcccaca atgccagcgg ttatttcact atttacggag
3061 acgaatccct ccagtcagat cacttcaact caagactaag ttttggagac acccagacca
3121 tctgggcacg cacaggctac ttggggttcc tgcggagaac tgagctcaca gatgcaaatg
3181 gagagcgcca tgatgccctc tacgtggtag gggcactgga cgaagccatg gagctgcggg
3241 gcatgcggta ccacccaatc gacattgaga cctcggtcat cagagcccat aaaagcgtta
3301 cggaatgtgc tgtgtttacc tggacaaatt tgttggtggt tgtggttgag ctggatgggt
3361 cggaacaaga agccttggac ctggttccct tggtgaccaa cgtggtcctg gaggagcact
3421 acctgatcgt cggagtggtg gtcgtggtgg acatcggcgt catccccatc aactcccgtg
3481 gggagaagca gcgcatgcac ctgcgagacg ggtttttggc agaccagcta gaccccatct
3541 atgtggccta caacatgtgt acagactaca aagaccatga cggtgattat aaagatcatg
3601 acatcgacta caaggatgac gatgacaagt aagaattctg atgactcgag ataatcaacc
3661 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
3721 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
3781 cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc
3841 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
3901 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa
3961 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
4021 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat
4081 ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
4141 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
4201 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
4261 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
4321 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
4381 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
4441 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
4501 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
4561 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
4621 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
4681 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
4741 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
4801 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4861 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
4921 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
4981 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
5041 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
5101 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
5161 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
5221 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
5281 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
5341 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
5401 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
5461 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
5521 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
5581 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
5641 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5701 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5761 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5821 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5881 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
5941 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
6001 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
6061 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
6121 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
6181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
6241 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
6301 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
6361 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6421 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
6481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
6541 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
6601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
6661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6721 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6781 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6841 ccttttgctc acatgt
//
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