| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-COL3A1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001654 |
| 产品用途: | 3' AAVLINK plasmid for COL3A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL3A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-COL3A1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-COL3A1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-COL3A1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-COL3A1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-COL3A1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-COL3A1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-COL3A1-FLAG 6666 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6666)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6666
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3379
/codon_start=1
/label=COL3A1
/translation="VKEALLELQDPLEVLDLLVLLVPKVSKVNVAVLVDLVLLASLVLV
VFLVLLVVMVTQDPQVPAVLQARMGPQVLRVTLVLLAALECLDQKVMLANQERRDRLVP
RAHQELQAHLGLLGSLEHGVLQDHQACQVLGEALALRVSRVKVGNQELTVSVENVVPLD
PRVFLVWLVQLVNLEEMETLDQMVFQAEMDLLVARVIVVKMALLVPLALLVIQAHLVLS
VQLERVVTEEKVALLALLVLPVLLVPEVLLVLKAHVVTKVKQVNVELLASKDIEDSLVI
QVPQVLQALLVSRVQSAVQDLQAPEDLLDPVDLLAKMEPVDIQVPLDHQGLEVTEVKED
LRAPQATQGNQALLDLLVPLVLAVVVLEPLPLLGLEVKKLAVLPRIMEMNQWISKSTPM
RL*LHSSLLMDK*KASLVLMVLVKTPLETAET*NSAILNSRVENTGLTLTKDANWMLSR
YSVIWKLGKHA*VPIL*MFHGNTGGQILVLRRNTFGLESPWMVVFSLATAILNFLKMSL
MCIWHSFDFSPAELPRTSHITAKIALHTWIRPVEM*RRP*S*WGQMKVNSRLKEIANSP
TQFWRMVARNTLGNGAKQSLNIEHARL*DYLL*ILHPMTLVVLIKNLVWTLALFAF"
misc_feature 1486..1491
/label=SDS
misc_feature 1493..1498
/label=SDS
misc_feature 1538..1543
/label=SDS
misc_feature 1628..1633
/label=SDS
misc_feature 2420..2425
/label=SDS
misc_feature 2537..2542
/label=SDS
misc_feature 2546..2551
/label=SDS
CDS 3386..3451
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3455..3886
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3599..3604
/label=SDS
repeat_region 3929..4069
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3929..4058
/label=AAV2 ITR
rep_origin 4144..4599
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4881..4985
/gene="bla"
/label=AmpR promoter
CDS 4986..5846
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6017..6605
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgaaggag gccctcctgg
1501 agttgcagga ccccctggag gttctggacc tgctggtcct cctggtcccc aaggtgtcaa
1561 aggtgaacgt ggcagtcctg gtggacctgg tgctgctggc ttccctggtg ctcgtggtct
1621 tcctggtcct cctggtagta atggtaaccc aggaccccca ggtcccagcg gttctccagg
1681 caaggatggg cccccaggtc ctgcgggtaa cactggtgct cctggcagcc ctggagtgtc
1741 tggaccaaaa ggtgatgctg gccaaccagg agagaaggga tcgcctggtg cccagggccc
1801 accaggagct ccaggcccac ttgggattgc tgggatcact ggagcacggg gtcttgcagg
1861 accaccaggc atgccaggtc ctaggggaag ccctggccct cagggtgtca agggtgaaag
1921 tgggaaacca ggagctaacg gtctcagtgg agaacgtggt ccccctggac cccagggtct
1981 tcctggtctg gctggtacag ctggtgaacc tggaagagat ggaaaccctg gatcagatgg
2041 tcttccaggc cgagatggat ctcctggtgg caagggtgat cgtggtgaaa atggctctcc
2101 tggtgcccct ggcgctcctg gtcatccagg cccacctggt cctgtcggtc cagctggaaa
2161 gagtggtgac agaggagaaa gtggccctgc tggccctgct ggtgctcccg gtcctgctgg
2221 ttcccgaggt gctcctggtc ctcaaggccc acgtggtgac aaaggtgaaa caggtgaacg
2281 tggagctgct ggcatcaaag gacatcgagg attccctggt aatccaggtg ccccaggttc
2341 tccaggccct gctggtcagc agggtgcaat cggcagtcca ggacctgcag gccccagagg
2401 acctgttgga cccagtggac ctcctggcaa agatggaacc agtggacatc caggtcccat
2461 tggaccacca gggcctcgag gtaacagagg tgaaagagga tctgagggct ccccaggcca
2521 cccagggcaa ccaggccctc ctggacctcc tggtgcccct ggtccttgct gtggtggtgt
2581 tggagccgct gccattgctg ggattggagg tgaaaaagct ggcggttttg ccccgtatta
2641 tggagatgaa ccaatggatt tcaaaatcaa caccgatgag attatgactt cactcaagtc
2701 tgttaatgga caaatagaaa gcctcattag tcctgatggt tctcgtaaaa accccgctag
2761 aaactgcaga gacctgaaat tctgccatcc tgaactcaag agtggagaat actgggttga
2821 ccctaaccaa ggatgcaaat tggatgctat caaggtattc tgtaatatgg aaactgggga
2881 aacatgcata agtgccaatc ctttgaatgt tccacggaaa cactggtgga cagattctag
2941 tgctgagaag aaacacgttt ggtttggaga gtccatggat ggtggttttc agtttagcta
3001 cggcaatcct gaacttcctg aagatgtcct tgatgtgcat ctggcattcc ttcgacttct
3061 ctccagccga gcttcccaga acatcacata tcactgcaaa aatagcattg catacatgga
3121 tcaggccagt ggaaatgtaa agaaggccct gaagctgatg gggtcaaatg aaggtgaatt
3181 caaggctgaa ggaaatagca aattcaccta cacagttctg gaggatggtt gcacgaaaca
3241 cactggggaa tggagcaaaa cagtctttga atatcgaaca cgcaaggctg tgagactacc
3301 tattgtagat attgcaccct atgacattgg tggtcctgat caagaatttg gtgtggacgt
3361 tggccctgtt tgctttttat gtacagacta caaagaccat gacggtgatt ataaagatca
3421 tgacatcgac tacaaggatg acgatgacaa gtaactcgag ataatcaacc tctggattac
3481 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
3541 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
3601 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
3661 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa
3721 atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc
3781 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
3841 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat ctggagaccg
3901 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
3961 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
4021 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
4081 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
4141 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
4201 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
4261 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4321 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
4381 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
4441 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
4501 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
4561 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
4621 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
4681 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
4741 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
4801 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
4861 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
4921 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
4981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
5041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
5101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
5161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
5221 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
5281 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
5341 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
5401 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
5461 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
5521 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
5581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
5641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
5701 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
5761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
5821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
5881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
5941 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
6001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
6061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
6121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
6181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
6241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
6301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
6361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
6421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
6481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
6541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
6601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
6661 acatgt
//
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