pLVX-DD-tdTomato Reporter

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-DD-tdTomato Reporter
质粒类型: 慢病毒载体;无启动子载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 9246 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: DD-tdTomato (C-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-DD-tdTomato-Reporter没有启动子元件;
DD-tdTomato与tdTomato相比,N端融合了ProteoTuner destabilization domain (DD)去稳定结构域,导致tdTomato在细胞内 很快被蛋白酶降解,降低了背景荧光,是研究启动子活性的理想报告基因。
产品目录号: 631754
稳定性: 稳表达
组成型/诱导型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-DD-tdTomato Reporter载体图谱



pLVX-DD-tdTomato Reporter 多克隆位点

pLVX-DD-tdTomato Reporter 载体特征

载体简介

载体序列

LOCUS       pLVX-DD-tdTomato_Reporter   9200 bp    DNA   circular             6-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631753,631754
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    5168..5759
                     /label=WPRE
     rep_origin      complement(7068..7741)
                     /label=pUC\origin
     CDS             complement(7886..8882)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     misc_feature    2028..2151
                     /label=cPPT/CTS
     LTR             5962..6598
                     /label=3'\LTR
     misc_feature    4026..4534
                     /label=PGK\Promoter
     misc_feature    4555..5154
                     /label=Puro(R)
     misc_feature    2195..2234
                     /label=MCS
     CDS             2247..2570
                     /label=DD
     CDS             2577..4004
                     /label=tdTomato
BASE COUNT     2262 a      2444 c      2487 g      2007 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgtcgagctc aagcttcgaa ttctgcagtc gacggtaccg 
     2221 cgggcccggg atcccgcgac tctagaatgg gagtgcaggt ggaaaccatc tccccaggag 
     2281 acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg 
     2341 aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag 
     2401 gcaagcagga ggtgatccga ggctgggaag aaggggttgc tcagatgagt gtgggtcaga 
     2461 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca 
     2521 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa agatctatgg 
     2581 tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct 
     2641 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 
     2701 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc 
     2761 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg 
     2821 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 
     2881 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca 
     2941 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca 
     3001 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga 
     3061 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct 
     3121 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca 
     3181 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc 
     3241 accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct 
     3301 cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg 
     3361 agggctccat gaacggccac gaattcgaga tcgagggcga gggcgagggc cgcccctacg 
     3421 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg 
     3481 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca 
     3541 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 
     3601 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga 
     3661 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga 
     3721 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg 
     3781 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga 
     3841 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc 
     3901 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg 
     3961 gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaggcg gccgcgactc 
     4021 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt 
     4081 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac 
     4141 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac 
     4201 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca 
     4261 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa 
     4321 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca 
     4381 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg 
     4441 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc 
     4501 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc 
     4561 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 
     4621 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 
     4681 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 
     4741 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 
     4801 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 
     4861 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 
     4921 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 
     4981 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 
     5041 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 
     5101 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt 
     5161 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 
     5221 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 
     5281 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 
     5341 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 
     5401 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 
     5461 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 
     5521 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 
     5581 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 
     5641 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 
     5701 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 
     5761 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca 
     5821 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca 
     5881 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 
     5941 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc 
     6001 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca 
     6061 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag 
     6121 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc 
     6181 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta 
     6241 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga 
     6301 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact 
     6361 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc 
     6421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
     6481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
     6541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 
     6601 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 
     6661 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat 
     6721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 
     6781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
     6841 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 
     6901 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     6961 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     7021 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     7081 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     7141 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     7201 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     7261 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     7321 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     7381 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     7441 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     7501 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     7561 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     7621 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     7681 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     7741 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     7801 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     7861 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     7921 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     7981 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     8041 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     8101 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     8161 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     8221 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     8281 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     8341 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     8401 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     8461 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     8521 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     8581 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     8641 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     8701 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     8761 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     8821 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     8881 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     8941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     9001 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat 
     9061 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta 
     9121 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata 
     9181 tgtactacaa acttagtagt 
//

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