| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001648 |
| 产品用途: | 3' AAVLINK plasmid for COL1A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL1A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG 6906 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6906)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6906
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3619
/codon_start=1
/label=COL1A1
/translation="VLMALLAKMASVV*PAPLVLLALLVPLVTRVKVVPAALLVPLELV
VPPETVVSLVPPALLALLAPLVLTANLVLKANLVMLVLKAMLVPLALPDPLDPLAPLVM
LVLLEPKVLAAALVPLVLLVSLVLLAESVLLAPLEMLDPLALLVLLAKKAAKVPVVRLA
LLDVLVKLVPLVPLALLARKDPLVLMVLLVLLVLPGLKVLLDSVVWSACLVREEREASL
VFLAPLVNLANKVPLEQVVNVVPLVPWAPLDWLDPLVNLDVRGLLVPKVPLDETVLLAP
RVTVVRPAPLDPLVLLVLLVPLAPLALLARVVIVVRLVLLVPPVLSALLAPVAPPDPKA
PVVTRVRQANRATEA*RVTVASLASRVPLALLALLVNKVPLEPLVLLVPEVPLALLVLL
AKMDSTVSLAPLGPLVLAVALVMLVLLVPPALLDLLVPLVLPALVSTSASCPSHLKRRL
TMVAATTGLMMPMWFVTVTSRWTPPSRA*ASRSRTSGAQRAAARTPPAPAVTSRCATLT
GRVESTGLTPTKAATWMPSKSSATWRLVRPACTPLSPVWPRRTGTSARTPRTRGMSGSA
RA*PMDSSSSMAARAPTLPMWPSS*PSCA*CPPRPPRTSPTTARTAWPTWTSRLATSRR
PCSSRAPTRSRSAPRATAASPTASLSMAARVTPEPGARQ*LNTKPPRPPACPSSMWPPW
TLVPQTRNSASTLALSAS"
misc_feature 1538..1543
/label=SDS
misc_feature 1880..1885
/label=SDS
misc_feature 1916..1921
/label=SDS
misc_feature 2591..2596
/label=SDS
misc_feature 2771..2776
/label=SDS
misc_feature 2780..2785
/label=SDS
CDS 3626..3691
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3695..4126
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3839..3844
/label=SDS
repeat_region 4169..4309
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4169..4298
/label=AAV2 ITR
rep_origin 4384..4839
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5121..5225
/gene="bla"
/label=AmpR promoter
CDS 5226..6086
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6257..6845
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgctgatg gctctcctgg
1501 caaagatggc gtccgtggtc tgaccggccc cattggtcct cctggccctg ctggtgcccc
1561 tggtgacaag ggtgaaagtg gtcccagcgg ccctgctggt cccactggag ctcgtggtgc
1621 ccccggagac cgtggtgagc ctggtccccc cggccctgct ggctttgctg gcccccctgg
1681 tgctgacggc caacctggtg ctaaaggcga acctggtgat gctggtgcta aaggcgatgc
1741 tggtccccct ggccctgccg gacccgctgg accccctggc cccattggta atgttggtgc
1801 tcctggagcc aaaggtgctc gcggcagcgc tggtccccct ggtgctactg gtttccctgg
1861 tgctgctggc cgagtcggtc ctcctggccc ctctggaaat gctggacccc ctggccctcc
1921 tggtcctgct ggcaaagaag gcggcaaagg tccccgtggt gagactggcc ctgctggacg
1981 tcctggtgaa gttggtcccc ctggtccccc tggccctgct ggcgagaaag gatcccctgg
2041 tgctgatggt cctgctggtg ctcctggtac tcccgggcct caaggtattg ctggacagcg
2101 tggtgtggtc ggcctgcctg gtcagagagg agagagaggc ttccctggtc ttcctggccc
2161 ctctggtgaa cctggcaaac aaggtccctc tggagcaagt ggtgaacgtg gtccccctgg
2221 tcccatgggc ccccctggat tggctggacc ccctggtgaa tctggacgtg agggggctcc
2281 tggtgccgaa ggttcccctg gacgagacgg ttctcctggc gccaagggtg accgtggtga
2341 gaccggcccc gctggacccc ctggtgctcc tggtgctcct ggtgcccctg gccccgttgg
2401 ccctgctggc aagagtggtg atcgtggtga gactggtcct gctggtcccg ccggtcctgt
2461 cggccctgtt ggcgcccgtg gccccgccgg accccaaggc ccccgtggtg acaagggtga
2521 gacaggcgaa cagggcgaca gaggcataaa gggtcaccgt ggcttctctg gcctccaggg
2581 tccccctggc cctcctggct ctcctggtga acaaggtccc tctggagcct ctggtcctgc
2641 tggtccccga ggtccccctg gctctgctgg tgctcctggc aaagatggac tcaacggtct
2701 ccctggcccc attgggcccc ctggtcctcg cggtcgcact ggtgatgctg gtcctgttgg
2761 tccccccggc cctcctggac ctcctggtcc ccctggtcct cccagcgctg gtttcgactt
2821 cagcttcctg ccccagccac ctcaagagaa ggctcacgat ggtggccgct actaccgggc
2881 tgatgatgcc aatgtggttc gtgaccgtga cctcgaggtg gacaccaccc tcaagagcct
2941 gagccagcag atcgagaaca tccggagccc agagggcagc cgcaagaacc ccgcccgcac
3001 ctgccgtgac ctcaagatgt gccactctga ctggaagagt ggagagtact ggattgaccc
3061 caaccaaggc tgcaacctgg atgccatcaa agtcttctgc aacatggaga ctggtgagac
3121 ctgcgtgtac cccactcagc ccagtgtggc ccagaagaac tggtacatca gcaagaaccc
3181 caaggacaag aggcatgtct ggttcggcga gagcatgacc gatggattcc agttcgagta
3241 tggcggccag ggctccgacc ctgccgatgt ggccatccag ctgaccttcc tgcgcctgat
3301 gtccaccgag gcctcccaga acatcaccta ccactgcaag aacagcgtgg cctacatgga
3361 ccagcagact ggcaacctca agaaggccct gctcctccag ggctccaacg agatcgagat
3421 ccgcgccgag ggcaacagcc gcttcaccta cagcgtcact gtcgatggct gcacgagtca
3481 caccggagcc tggggcaaga cagtgattga atacaaaacc accaagacct cccgcctgcc
3541 catcatcgat gtggccccct tggacgttgg tgccccagac caggaattcg gcttcgacgt
3601 tggccctgtc tgcttcctgt gtacagacta caaagaccat gacggtgatt ataaagatca
3661 tgacatcgac tacaaggatg acgatgacaa gtaactcgag ataatcaacc tctggattac
3721 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
3781 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
3841 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
3901 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa
3961 atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc
4021 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
4081 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat ctggagaccg
4141 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
4201 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
4261 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
4321 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
4381 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
4441 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
4501 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4561 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
4621 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
4681 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
4741 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
4801 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
4861 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
4921 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
4981 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
5041 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
5101 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
5161 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
5221 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
5281 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
5341 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
5401 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
5461 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
5521 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
5581 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
5641 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
5701 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
5761 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
5821 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
5881 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
5941 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
6001 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
6061 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
6121 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
6181 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
6241 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
6301 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
6361 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
6421 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
6481 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
6541 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
6601 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
6661 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
6721 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
6781 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
6841 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
6901 acatgt
//
pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG载体图谱质粒图谱pdf版和pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv1-pCALM1-Cre-3'-COL1A1-FLAG质粒载体应用举例
- 上一篇：pAAVLINK-EF1a-5'-COL1A1
- 下一篇：没有了
