pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

LOCUS       pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)                9864 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively 
            edit adenosine to inosine in RNA molecules when used in conjuction 
            with a guide RNA. Longer linker than pC0039..
ACCESSION   .
VERSION     .
KEYWORDS    pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung 
            J, Zhang F
  TITLE     RNA editing with CRISPR-Cas13.
  JOURNAL   Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
  PUBMED    29070703
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        18..347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     260..279
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             414..1208
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(468..487)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1078..1097
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1382..1503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1419..1438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1473..1492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1552..1568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1552..1568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1565..1587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1576..1592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1600..1630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1645..1666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1783..1800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1954..2542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2034..2053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2713..3573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3336..3355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3574..3678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3753..3772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3944..4323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4324..4527
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4478..4498
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4572..4591
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4572..4590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7914..7940
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(9164..9181)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9170..9394
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9440..4
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9527..9546)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9737..9758
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
      361 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
      421 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
      481 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
      541 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
      601 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
      661 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
      721 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
      781 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
      841 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
      901 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
      961 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     1021 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     1081 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     1141 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     1201 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     1261 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     1321 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     1381 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1501 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     1561 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1621 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1681 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1801 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1861 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2641 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3301 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     3721 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3781 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     3841 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     3901 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     3961 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     4021 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     4081 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     4141 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     4201 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     4261 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4321 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4381 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4441 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4501 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     4561 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta
     4621 agcttgccac catgaacatc cccgctctgg tggaaaacca gaagaagtac tttggcacct
     4681 acagcgtgat ggccatgctg aacgctcaga ccgtgctgga ccacatccag aaggtggccg
     4741 atattgaggg cgagcagaac gagaacaacg agaatctgtg gtttcacccc gtgatgagcc
     4801 acctgtacaa cgccaagaac ggctacgaca agcagcccga gaaaaccatg ttcatcatcg
     4861 agcggctgca gagctacttc ccattcctga agatcatggc cgagaaccag agagagtaca
     4921 gcaacggcaa gtacaagcag aaccgcgtgg aagtgaacag caacgacatc ttcgaggtgc
     4981 tgaagcgcgc cttcggcgtg ctgaagatgt acagggacct gaccaacgca tacaagacct
     5041 acgaggaaaa gctgaacgac ggctgcgagt tcctgaccag cacagagcaa cctctgagcg
     5101 gcatgatcaa caactactac acagtggccc tgcggaacat gaacgagaga tacggctaca
     5161 agacagagga cctggccttc atccaggaca agcggttcaa gttcgtgaag gacgcctacg
     5221 gcaagaaaaa gtcccaagtg aataccggat tcttcctgag cctgcaggac tacaacggcg
     5281 acacacagaa gaagctgcac ctgagcggag tgggaatcgc cctgctgatc tgcctgttcc
     5341 tggacaagca gtacatcaac atctttctga gcaggctgcc catcttctcc agctacaatg
     5401 cccagagcga ggaacggcgg atcatcatca gatccttcgg catcaacagc atcaagctgc
     5461 ccaaggaccg gatccacagc gagaagtcca acaagagcgt ggccatggat atgctcaacg
     5521 aagtgaagcg gtgccccgac gagctgttca caacactgtc tgccgagaag cagtcccggt
     5581 tcagaatcat cagcgacgac cacaatgaag tgctgatgaa gcggagcagc gacagattcg
     5641 tgcctctgct gctgcagtat atcgattacg gcaagctgtt cgaccacatc aggttccacg
     5701 tgaacatggg caagctgaga tacctgctga aggccgacaa gacctgcatc gacggccaga
     5761 ccagagtcag agtgatcgag cagcccctga acggcttcgg cagactggaa gaggccgaga
     5821 caatgcggaa gcaagagaac ggcaccttcg gcaacagcgg catccggatc agagacttcg
     5881 agaacatgaa gcgggacgac gccaatcctg ccaactatcc ctacatcgtg gacacctaca
     5941 cacactacat cctggaaaac aacaaggtcg agatgtttat caacgacaaa gaggacagcg
     6001 ccccactgct gcccgtgatc gaggatgata gatacgtggt caagacaatc cccagctgcc
     6061 ggatgagcac cctggaaatt ccagccatgg ccttccacat gtttctgttc ggcagcaaga
     6121 aaaccgagaa gctgatcgtg gacgtgcaca accggtacaa gagactgttc caggccatgc
     6181 agaaagaaga agtgaccgcc gagaatatcg ccagcttcgg aatcgccgag agcgacctgc
     6241 ctcagaagat cctggatctg atcagcggca atgcccacgg caaggatgtg gacgccttca
     6301 tcagactgac cgtggacgac atgctgaccg acaccgagcg gagaatcaag agattcaagg
     6361 acgaccggaa gtccattcgg agcgccgaca acaagatggg aaagagaggc ttcaagcaga
     6421 tctccacagg caagctggcc gacttcctgg ccaaggacat cgtgctgttt cagcccagcg
     6481 tgaacgatgg cgagaacaag atcaccggcc tgaactaccg gatcatgcag agcgccattg
     6541 ccgtgtacga tagcggcgac gattacgagg ccaagcagca gttcaagctg atgttcgaga
     6601 aggcccggct gatcggcaag ggcacaacag agcctcatcc atttctgtac aaggtgttcg
     6661 cccgcagcat ccccgccaat gccgtcgagt tctacgagcg ctacctgatc gagcggaagt
     6721 tctacctgac cggcctgtcc aacgagatca agaaaggcaa cagagtggat gtgcccttca
     6781 tccggcggga ccagaacaag tggaaaacac ccgccatgaa gaccctgggc agaatctaca
     6841 gcgaggatct gcccgtggaa ctgcccagac agatgttcga caatgagatc aagtcccacc
     6901 tgaagtccct gccacagatg gaaggcatcg acttcaacaa tgccaacgtg acctatctga
     6961 tcgccgagta catgaagaga gtgctggacg acgacttcca gaccttctac cagtggaacc
     7021 gcaactaccg gtacatggac atgcttaagg gcgagtacga cagaaagggc tccctgcagc
     7081 actgcttcac cagcgtggaa gagagagaag gcctctggaa agagcgggcc tccagaacag
     7141 agcggtacag aaagcaggcc agcaacaaga tccgcagcaa ccggcagatg agaaacgcca
     7201 gcagcgaaga gatcgagaca atcctggata agcggctgag caacagccgg aacgagtacc
     7261 agaaaagcga gaaagtgatc cggcgctaca gagtgcagga tgccctgctg tttctgctgg
     7321 ccaaaaagac cctgaccgaa ctggccgatt tcgacggcga gaggttcaaa ctgaaagaaa
     7381 tcatgcccga cgccgagaag ggaatcctga gcgagatcat gcccatgagc ttcaccttcg
     7441 agaaaggcgg caagaagtac accatcacca gcgagggcat gaagctgaag aactacggcg
     7501 acttctttgt gctggctagc gacaagagga tcggcaacct gctggaactc gtgggcagcg
     7561 acatcgtgtc caaagaggat atcatggaag agttcaacaa atacgaccag tgcaggcccg
     7621 agatcagctc catcgtgttc aacctggaaa agtgggcctt cgacacatac cccgagctgt
     7681 ctgccagagt ggaccgggaa gagaaggtgg acttcaagag catcctgaaa atcctgctga
     7741 acaacaagaa catcaacaaa gagcagagcg acatcctgcg gaagatccgg aacgccttcg
     7801 atgcaaacaa ttaccccgac aaaggcgtgg tggaaatcaa ggccctgcct gagatcgcca
     7861 tgagcatcaa gaaggccttt ggggagtacg ccatcatgaa gggaagcctg cagctgcctc
     7921 cacttgaaag actgacactg ggatccggag gaggtggaag ccagctgcat ttaccgcagg
     7981 ttttagctga cgctgtctca cgcctggtcc tgggtaagtt tggtgacctg accgacaact
     8041 tctcctcccc tcacgctcgc agaaaagtgc tggctggagt cgtcatgaca acaggcacag
     8101 atgttaaaga tgccaaggtg ataagtgttt ctacaggaac aaaatgtatt aatggtgaat
     8161 acatgagtga tcgtggcctt gcattaaatg actgccatgc agaaataata tctcggagat
     8221 ccttgctcag atttctttat acacaacttg agctttactt aaataacaaa gatgatcaaa
     8281 aaagatccat ctttcagaaa tcagagcgag gggggtttag gctgaaggag aatgtccagt
     8341 ttcatctgta catcagcacc tctccctgtg gagatgccag aatcttctca ccacatgagc
     8401 caatcctgga agaaccagca gatagacacc caaatcgtaa agcaagagga cagctacgga
     8461 ccaaaataga gtctggtcag gggacgattc cagtgcgctc caatgcgagc atccaaacgt
     8521 gggacggggt gctgcaaggg gagcggctgc tcaccatgtc ctgcagtgac aagattgcac
     8581 gctggaacgt ggtgggcatc cagggatcac tgctcagcat tttcgtggag cccatttact
     8641 tctcgagcat catcctgggc agcctttacc acggggacca cctttccagg gccatgtacc
     8701 agcggatctc caacatagag gacctgccac ctctctacac cctcaacaag cctttgctca
     8761 gtggcatcag caatgcagaa gcacggcagc cagggaaggc ccccaacttc agtgtcaact
     8821 ggacggtagg cgactccgct attgaggtca tcaacgccac gactgggaag gatgagctgg
     8881 gccgcgcgtc ccgcctgtgt aagcacgcgt tgtactgtcg ctggatgcgt gtgcacggca
     8941 aggttccctc ccacttacta cgctccaaga ttaccaagcc caacgtgtac catgagtcca
     9001 agctggcggc aaaggagtac caggccgcca aggcgcgtct gttcacagcc ttcatcaagg
     9061 cggggctggg ggcctgggtg gagaagccca ccgagcagga ccagttctca ctcacgtaag
     9121 cggccgctcg agcctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     9181 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     9241 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9301 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     9361 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     9421 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9481 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9541 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9601 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9661 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9721 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9781 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9841 ctgatttaac aaaaatttaa cgcg
//