| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-CECR2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001480 |
| 产品用途: | 3' AAVLINK plasmid for CECR2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CECR2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-CECR2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-CECR2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-CECR2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-CECR2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-CECR2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-CECR2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-CECR2-FLAG 6660 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6660)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6660
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3361
/codon_start=1
/label=CECR2
/translation="GTCDRPASLPDIGYSHLQCQHPVLCLEHLPRLFGGCREGTP*WTA
QR*LRCSSSPPASAPQVCLTTPTSLHTPVYLALFRR*LTQCQSLCQPPSLPWATLGGHR
RTVKHKSLRMTKQSRCLALKRNHQVLVLQRGSTSHNYLTPHLPCRLTAPGRAHHKKGKQ
WARSSKAAPPNLRTTVKQ*RARIPGPRIAATPAQPPKGA*ETSPRWQTGALYPRTESLG
KHLLVDRRGRALVAVVPKSCSAPEAERCRKPCHARDRTQRHRPAQTPV*REAL*ASFPR
CICLA*STRIQLPITTSVQACRVWAL*WEGSPQHPIPSIFPQGAFSLTTHILEAFPGIA
PHKE*GIPTTHRHSLPTTTISELLTMPVHRAFLTGRDLSIPREAQADPQPVSLPHQGPS
SQIRMPWPVCKAVRH*MLP*LLQPVWMQWLLKSQMTGRILVQRKRSWMNLWRGQRVPKN
F*TWTTITQLPSGRARCQPASISMELLRL*VQEWDLVHLHFHPTV*CCRRGLPIPLSGR
PVTFSPGLTLPLWLPSHLTTQGPPSPTASLRRVPSIAARKKAWVTFKL**WNKLALEVE
*EDLSRKCTDHQECRCTRSSRRPRSQRPPQQQHHRRRCRLISLQHFPWIR"
misc_feature 3123..3128
/label=SDS
misc_feature 3312..3317
/label=SDS
misc_feature 3315..3320
/label=SDS
CDS 3368..3433
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3449..3880
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3593..3598
/label=SDS
repeat_region 3923..4063
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3923..4052
/label=AAV2 ITR
rep_origin 4138..4593
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4875..4979
/gene="bla"
/label=AmpR promoter
CDS 4980..5840
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6011..6599
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggtacatgc gaccgccctg
1501 caagtctgcc ggacatcggt tacagccacc tccagtgcca gcacccagtt ctttgtttgg
1561 agcacctgcc caggctcttc ggggggtgca gggaggggac tccatgatgg acagcccaga
1621 gatgattgcg atgcagcagc tctcctcccg cgtctgcccc ccaggtgtgc cttaccaccc
1681 ccaccagcct gcacaccccc gtttacctgg cccttttccg caggtagctc acccaatgtc
1741 agtcactgtg tcagccccca agcctgccct gggcaaccct gggagggcac cggagaacag
1801 tgaagcacaa gagcctgaga atgaccaagc agagccgttg cctggccttg aagagaaacc
1861 accaggtgtt ggtacttcag agggggtcta cctcacacaa ctacctcacc ccacacctcc
1921 cctgcagact gactgcacca ggcagagctc accacaagaa agggaaacag tgggcccgga
1981 gctcaaaagc agctcctccg aatctgcgga caactgtaaa gcaatgaagg gcaagaatcc
2041 ctggccctcg gatagcagct accccggccc agccgcccaa gggtgcgtga gagacctctc
2101 cacggtggca gacaggggcg ctctatccga gaacggagtc attggggaag catctccttg
2161 tggatcggag gggaagggcc ttggtagcag tggttccgaa aagctgctct gccccagagg
2221 cagaacgttg caggaaacca tgccatgcac gggacagaac gcagcgacac cgcccagcac
2281 agaccccggt ttgacgggag gcactgtgag ccagtttccc ccgctgtata tgcctggcct
2341 agagtacccg aattcagctg cccattacca catcagtcca ggcctgcagg gtgtgggccc
2401 tgtgatggga gggaagtccc cagcatccca tccccagcat tttcccccaa ggggctttca
2461 gtctaaccac ccacattctg gaggctttcc ccggtatcgc cccccacaag gaatgaggta
2521 ttcctaccac ccaccgccac agccttccta ccaccactat cagcgaactc cttactatgc
2581 ctgtccacag agcttttctg actggcagag acctctccat ccccagggaa gcccaagcgg
2641 acccccagcc agtcagcctc ccccaccaag gtccctcttc tcagataaga atgccatggc
2701 cagtctgcaa ggctgtgaga cactgaatgc tgccttaact tctccaaccc gtatggatgc
2761 agtggctgct aaagtcccaa atgacgggca gaatcctggt ccagaggaag agaagctgga
2821 tgaatctatg gagaggccag agagtcccaa agaattttta gacctggaca accataacgc
2881 agctaccaag cggcagagct cgttgtcagc cagcgagtat ctctatggaa ctcctccgcc
2941 tctgagttca ggaatgggat ttggttcatc tgcatttcca ccccacagtg tgatgctgca
3001 gacggggcct ccctataccc ctcagcggcc ggccagtcac tttcagccca gggcttactc
3061 ttcccctgtg gctgccctcc cacctcacca cccaggggcc acccagccca acggcctctc
3121 tcaggagggt cccatctatc gctgccagga agaaggcctg ggtcactttc aagctgtgat
3181 gatggaacaa attggcacta gaagtggaat aagaggacct ttccaggaaa tgtacagacc
3241 atcaggaatg cagatgcacc cggtccagtc gcaggcctcg ttcccaaaga cccccacagc
3301 agcaacatca caggaggagg tgccgcctca taagcctcca acacttcccc tggatcagag
3361 ctgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
3421 tgacgatgac aagtaagaat tctgatgact cgagataatc aacctctgga ttacaaaatt
3481 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
3541 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
3601 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
3661 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt
3721 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc
3781 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
3841 tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg
3901 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
3961 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
4021 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
4081 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
4141 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
4201 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
4261 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
4321 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
4381 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
4441 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
4501 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
4561 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
4621 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
4681 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
4741 tgtgtcagag gttttcaccg tcatcaccgg aacgcgcgag acgaaagggc ctcgtgatac
4801 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
4861 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
4921 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
4981 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
5041 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
5101 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
5161 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
5221 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
5281 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
5341 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
5401 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
5461 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
5521 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
5581 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
5641 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
5701 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
5761 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
5821 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
5881 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
5941 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
6001 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
6061 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
6121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
6181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
6241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
6301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
6361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
6421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
6481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
6541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
6601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
