| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-ATP10A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001486 |
| 产品用途: | 3' AAVLINK plasmid for ATP10A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ATP10A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-ATP10A-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ATP10A-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ATP10A-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ATP10A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ATP10A-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ATP10A-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ATP10A-FLAG 6967 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6967)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6967
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3680
/codon_start=1
/label=ATP10A
/translation="GADSVVMDLLQPCSSVDARGRHQKKIRSKTQNYLNVYAAEGLRTL
CIAKRVLSKEEYACWLQSHLEAESSLENSEELLFQSAIRLETNLHLLGATGIEDRLQDG
VPETISKLRQAGLQIWVLTGDKQETAVNIAYACKLLDHDEEVITLNATSQEACAALLDQ
CLCYVQSRGLQRAPEKTKGKVSMRFSSLCPPSTSTASGRRPSLVIDGRSLAYALEKNLE
DKFLFLAKQCRSVLCCRSTPLQKSMVVKLVRSKLKAMTLAIGDGANDVSMIQVADVGVG
ISGQEGMQAVMASDFAVPKFRYLERLLILHGHWCYSRLANMVLYFFYKNTMFVGLLFWF
QFFCGFSASTMIDQWYLIFFNLLFSSLPPLVTGVLDRDVPANVLLTNPQLYKSGQNMEE
YRPRTFWFNMADAAFQSLVCFSIPYLAYYDSNVDLFTWGTPIVTIALLTFLLHLGIETK
TWTWLNWITCGFSVLLFFTVALIYNASCATCYPPSNPYWTMQALLGDPVFYLTCLMTPV
AALLPRLFFRSLQGRVFPTQLQLARQLTRKSPRRCSAPKETFAQGRLPKDSGTEHSSGR
TVKTSVPLSQPSWHTQQPVCSLEASGEPSTVDMSMPVREHTLLEGLSAPAPMSSAPGEA
VLRSPGGCPEESKVRAASTGRVTPLSSLFSLPTFSLLNWISSWSLVSRLGSVLQFSRTE
QLADGQAGRGLPVQPHSGRSGLQGPDHRLLIGASSRRSQ"
misc_feature 1912..1917
/label=SDS
misc_feature 1942..1947
/label=SDS
misc_feature 2335..2340
/label=SDS
misc_feature 2486..2491
/label=SDS
misc_feature 3401..3406
/label=SDS
misc_feature 3508..3513
/label=SDS
CDS 3687..3752
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3756..4187
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3900..3905
/label=SDS
repeat_region 4230..4370
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4230..4359
/label=AAV2 ITR
rep_origin 4445..4900
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5182..5286
/gene="bla"
/label=AmpR promoter
CDS 5287..6147
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6318..6906
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggggccgac tcagtggtca
1501 tggatctcct gcagccctgc tcttcagttg acgccagagg gaggcatcaa aaaaagattc
1561 ggagcaaaac tcagaattac ctcaacgtgt atgcggcgga aggcctgcgc accttgtgca
1621 tcgccaagag agttctgagt aaagaagagt atgcctgctg gttgcaaagc cacctagaag
1681 ccgaatcctc cctggaaaac agcgaggagc tcctcttcca gtctgccatt cgcctggaga
1741 ccaacctgca cttgttaggt gccactggga ttgaagaccg cctgcaggac ggagtccctg
1801 aaactatttc taaattgcgt caagcgggcc tgcagatttg ggttctcact ggtgacaaac
1861 aagaaacagc tgtcaacatt gcatatgcct gcaaactgct ggaccacgac gaggaggtca
1921 tcaccctgaa tgccacctcc caggaggcgt gtgcagccct gctagaccag tgcctatgct
1981 acgtgcagtc cagaggcctc cagagagccc ctgagaagac caagggcaaa gtgagcatga
2041 ggttctcctc tctctgccca ccctccacgt ccactgcctc tggccgcaga cccagcctcg
2101 tgatcgatgg gagaagcctg gcctacgctc tcgagaaaaa cctggaggac aaattcctct
2161 tccttgccaa gcagtgccgc tccgtcctct gctgtcggtc gacgcctctg cagaagagca
2221 tggtggtgaa gctggtgcgg agcaagctca aggccatgac cctggccata ggtgatggag
2281 ccaatgatgt cagcatgatc caggtggcag atgtgggtgt gggaatctcc ggccaggagg
2341 gtatgcaggc agtgatggcc agcgactttg cagtgccgaa attccgatac ctggagaggc
2401 tcttgattct tcacgggcat tggtgctact cccgacttgc caacatggtg ctgtacttct
2461 tctacaaaaa cacaatgttc gtgggcctcc tgttttggtt ccagtttttc tgtggcttct
2521 ctgcatctac catgattgac cagtggtatc taatcttctt taatctgctc ttctcgtcac
2581 ttcccccgct cgtgactggg gtgctggaca gggatgtgcc agccaatgtg ctgctgacca
2641 acccgcagct ctacaagagt ggccagaaca tggaggaata ccggccacga acgttctggt
2701 ttaacatggc cgacgccgcc ttccagagcc tggtttgctt ttccattcct tacctggcct
2761 actatgactc gaacgtggac ctgtttacct gggggacccc tattgtgaca atcgcgctgc
2821 tcactttcct gctccacctg ggcattgaaa ccaaaacctg gacctggctc aactggataa
2881 cgtgtggctt cagtgtcctt ttgtttttca ccgtggcttt gatttacaat gcgtcttgtg
2941 ccacgtgcta tcctccgtcc aacccttact ggactatgca agccttactg ggtgacccag
3001 tgttttactt gacttgcctg atgacgcctg tcgctgcact gctgcccaga ttgtttttca
3061 gatccctcca ggggagggtt ttccccacac aacttcagct ggcacgtcag ttgaccagga
3121 agtcccccag gagatgcagt gctcccaaag agacctttgc tcagggacgc ctcccgaagg
3181 actcgggaac cgagcactca tcagggagga cagtcaagac ctctgtgccc ctgtcccagc
3241 cttcttggca cacacagcag ccggtctgct ccctggaggc cagcggggag cccagcacag
3301 tggacatgag catgccagtg agggagcaca ccctgctgga ggggctgagc gcaccggccc
3361 ccatgtcctc tgcgccaggg gaggctgtcc tgaggagtcc aggagggtgt cctgaggagt
3421 ccaaggtgag agctgccagc accggcaggg tgacccccct gtcttccctc ttcagcctgc
3481 ctaccttcag cttactcaac tggatttcct cctggtcgct ggtcagcagg ctggggagtg
3541 tcttacagtt ctcccggacg gagcagcttg cagatggaca agcgggacgt ggacttcctg
3601 tccagcccca ctcaggccga tcaggacttc aagggccaga ccacagacta cttataggag
3661 catcttcaag gcggtcacag tgtacagact acaaagacca tgacggtgat tataaagatc
3721 atgacatcga ctacaaggat gacgatgaca agtaactcga gataatcaac ctctggatta
3781 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
3841 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
3901 ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc
3961 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga
4021 aatttgtgat gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg
4081 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
4141 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc
4201 ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
4261 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
4321 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
4381 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
4441 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4501 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
4561 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
4621 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
4681 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
4741 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
4801 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
4861 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
4921 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
4981 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
5041 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
5101 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
5161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
5221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
5281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
5341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
5401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
5461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
5521 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
5581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
5641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
5701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
5761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
5821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
5881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
5941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
6001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
6061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
6121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
6181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
6241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
6301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
6361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
6421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
6481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
6541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
6601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
6661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
6721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
6781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
6841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
6901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
6961 cacatgt
//
