| 质粒名称: |
pAAVLINKv1-pCALM1-Cre-3'-ASTN2-HA
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001471 |
| 产品用途: | 3' AAVLINK plasmid for ASTN2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ASTN2 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-ASTN2-HA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ASTN2-HA载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ASTN2-HA质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ASTN2-HA载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ASTN2-HA质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ASTN2-HA质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ASTN2-HA 7092 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7092)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7092
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3775
/codon_start=1
/label=ASTN2
/translation="GSKPLKRFSGLLSAWAKASGFLSAKALWFRLWSCPSTPWPAARPM
CSSRKTLQMSGKKRCCPHTLKPSMTCCLPSGQFVTALGTMGAALATSSVCLTGRWIPRD
VCALRS*NP*RMALAATTTPKALTALMALMAAVSSCACSRRCPCPTMPLRAPSSCSAVA
WRSTNWLLMENPA*CSQMSARAPSASNLTPNSMIPSLERCYMVTTTGPSM*TKAKSSR*
PLGRTTSSRTFPSWPMGCW*SRCRWRSSAGGSSPSPFRTSNCSLEISGMMRPWVTPWCS
SGGSGATSTV*SSAPSPSQQASLMFSRS*PRRAVGRSCCPSSSTMAPTTSQRPSMAQSS
PASSTFPARRSSSSCGSSIRKRPQSWAARRSSSPCPSSPTSQVC*QPRCCQMTSSFQVW
RFAVRRRGAVHLPVTFAAGQARSS*APHQCCWKSTVWCHFIPSSKTMAQRRPSRVH**V
PTGAQGKGM*SMTGAGVTSAPLMPMGSPTAAPFCSRCCGCPQQWSPPVLWSPWSGWMFS
QLLGPRSPTIFCSIRKWMNTQTLTCTQENS*VLLMTYSLAWAHLV*QLVEAMERSLKSV
STQSSSSVWSPTVSTSSLCMLWIHEGGTQS*AR*P*GRPVHW*MTTRQKK*LTRSTICT
MGTQVERSSRWPTTH*WRSQPRCCSESSTTTTLTMKSLATSSGEVRMSWGPGRPT*FYG
DWRG*VATAPASCGVPTSRAAWKQCPIFSAAARRSGLQAWCGIASSRTPKSRVRRRWCQ
WPETRTGSPRA"
misc_feature 2826..2831
/label=SDS
misc_feature 3595..3600
/label=SDS
misc_feature 3660..3665
/label=SDS
CDS 3791..3817
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3821..3847
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3851..3877
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
terminator 3881..4312
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4025..4030
/label=SDS
repeat_region 4355..4495
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4355..4484
/label=AAV2 ITR
rep_origin 4570..5025
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5307..5411
/gene="bla"
/label=AmpR promoter
CDS 5412..6272
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6443..7031
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggagcaag cccctgaaaa
1501 gattctcagg tctactttca gcttgggcca aggcctctgg cttcctgtca gcaaaagctt
1561 tgtggttccg cctgtggagc tgtccatcaa ccccctggcc agctgcaaga ccgatgtgct
1621 cgtcacggaa gaccctgcag atgtcaggga agaagcgatg ctgtccacat actttgaaac
1681 catcaatgac ctgctgtctt ccttcgggcc agttcgtgac tgctctcgga acaatggggg
1741 ctgcactcgc aacttcaagt gtgtgtctga ccggcaggtg gattcctcgg gatgtgtgtg
1801 ccctgaggag ctgaaaccca tgaaggatgg ctctggctgc tacgaccact ccaaaggcat
1861 tgactgctct gatggcttta atggcggctg tgagcagctg tgcctgcagc agacgctgcc
1921 cctgccctac gatgccactt cgagcaccat cttcatgttc tgcggttgcg tggaggagta
1981 caaactggct cctgatggaa aatcctgctt aatgctctca gatgtctgcg agggccccaa
2041 gtgcctcaaa cctgactcca aattcaatga taccctcttt ggagagatgc tacatggtta
2101 caacaaccgg acccagcatg tgaaccaagg ccaagtcttc cagatgacct ttagggagaa
2161 caacttcatc aaggactttc cccagctggc cgatgggctg ttggtgatcc cgctgccggt
2221 ggaggagcag tgccgggggg tcctctccga gccccttccg gacctccaac tgctcactgg
2281 agatatcagg tatgatgagg ccatgggtta ccccatggtg cagcagtggc gggtccggag
2341 caacctctac cgtgtgaagc tcagcaccat caccctcgca gcaggcttca ctaatgttct
2401 caagatcctg accaaggaga gcagtcggga ggagctgctg tccttcatcc agcactatgg
2461 ctcccactac atcgcagagg ccctctatgg ctcagagctc acctgcatca tccactttcc
2521 cagcaagaag gtccagcagc agctgtggct ccagtatcag aaagagacca cagagctggg
2581 cagcaagaag gagctcaagt ccatgccctt catcacctac ctctcaggtt tgctgacagc
2641 ccagatgctg tcagatgacc agctcatttc aggtgtggag attcgctgtg aggagaaggg
2701 gcgctgtcca tctacctgtc acctttgccg ccggccaggc aaggagcagc tgagccccac
2761 accagtgctg ctggaaatca accgtgtggt gccactttat accctcatcc aagacaatgg
2821 cacaaaggag gccttcaaga gtgcactgat gagttcctac tggtgctcag ggaaagggga
2881 tgtgatcgat gactggtgca ggtgtgacct cagcgccttt gatgccaatg ggctccccaa
2941 ctgcagcccc cttctgcagc cggtgctgcg gctgtcccca acagtggagc cctccagtac
3001 tgtggtctcc ttggagtggg tggatgttca gccagctatt gggaccaagg tctccgacta
3061 tattctgcag cataagaaag tggatgaata cacagacact gacctgtaca caggagaatt
3121 cctgagtttt gctgatgact tactctctgg cctgggcaca tcttgtgtag cagctggtcg
3181 aagccatgga gaggtccctg aagtcagtat ctactcagtc atcttcaagt gtctggagcc
3241 cgacggtctc tacaagttca ctctgtatgc tgtggataca cgagggaggc actcagagct
3301 aagcacggtg accctgagga cggcctgtcc actggtagat gacaacaagg cagaagaaat
3361 agctgacaag atctacaatc tgtacaatgg gtacacaagt ggaaaggagc agcagatggc
3421 ctacaacaca ctgatggagg tctcagcctc gatgctgttc cgagtccagc accactacaa
3481 ctctcactat gaaaagtttg gcgacttcgt ctggagaagt gaggatgagc tggggcccag
3541 gaaggcccac ctgattctac ggcgactgga gagggtgagt agccactgct ccagcctcct
3601 gcggagtgcc tacatccaga gccgcgtgga aacagtgccc tatcttttct gccgcagcga
3661 ggaggtccgg cctgcaggca tggtgtggta tagcatcctc aaggacacca aaatcacgtg
3721 tgaggagaag atggtgtcaa tggcccgaaa cacgtacggg gagtccaagg gccggggagg
3781 tggatctggt tacccgtatg atgttccgga ttacgctggc tatccctacg acgtgcccga
3841 ctatgccggg tacccctatg acgtcccaga ctacgcataa ctcgagataa tcaacctctg
3901 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
3961 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
4021 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
4081 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt
4141 tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg
4201 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
4261 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg
4321 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
4381 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
4441 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
4501 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
4561 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
4621 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
4681 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
4741 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
4801 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
4861 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
4921 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
4981 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
5041 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
5101 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
5161 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
5221 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
5281 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
5341 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
5401 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
5461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
5521 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
5581 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
5641 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
5701 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
5761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
5821 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
5881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
5941 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
6001 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
6061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
6121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
6181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
6241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
6301 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
6361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
6421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
6481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
6541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
6601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
6661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
6841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
6901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
6961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
7021 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
7081 ttgctcacat gt
//
