| 质粒名称: | pAAVLINK-EF1a-HA-5'-YEATS2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001464 |
| 产品用途: | 5' AAVLINK plasmid for YEATS2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | YEATS2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-YEATS2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-YEATS2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-YEATS2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-YEATS2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-YEATS2质粒载体简介
pAAVLINK-EF1a-HA-5'-YEATS2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-YEATS2 5770 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5770)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5770
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..2932
/codon_start=1
/label=YEATS2
/translation="SGIKRTIKETDPDYEDVSVALPNKRHKAIENSARDAAVQKIETII
KEQFALEMKNKEHEIEVIDQRLIEARRMMDKLRACIVANYYASAGLLKVSEGSKTCDTM
VFNHPAIKKFLESPSRSSSPANQRAETPSANHSESDSLSQHNDFLSDKDNNSNMDIEER
LSNNMEQRPSRNTGRDTSRITGSHKTEQRNADLTDETSRLFVKKTIVVGNVSKYIPPDK
REENDQSTHKWMVYVRGSRREPSINHFVKKVWFFLHPSYKPNDLVEVREPPFHLTRRGW
GEFPVRVQVHFKDSQNKRIDIIHNLKLDRTYTGLQTLGAETVVDVELHRHSLGEDCIYP
QSSESDISDAPPSLPLTIPAPVKASSPIKQSHEPVPDTSVEKGFPASTEAERHTPFYAL
PSSLERTPTKMTTSQKVTFCSHGNSAFQPIASSCKIVPQSQVPNPESPGKSFQPITMSC
KIVSGSPISTPSPSPLPRTPTSTPVHVKQGTAGSVINNPYVIMDKQPGQVIGATTPSTG
SPTNKISTASQVSQGTGSPVPKIHGSSFVTSTVKQEDSLFASMPPLCPIGSHPKVQSPK
PITGGLGAFTKVIIKQEPGEAPHVPATGAASQSPLPQYVTVKGGHMIAVSPQKQVITPG
EGIAQSAKVQPSKVVGVPVGSALPSTVKQAVAISGGQILVAKASSSVSKAVGPKQVVTQ
GVAKAIVSGGGGTIVAQPVQTLTKAQVTAAGPQKSGSQ"
misc_feature 1550..1555
/label=SDS
misc_feature 2391..2396
/label=SDS
misc_feature 2473..2478
/label=SDS
intron 2933..2973
/label=Splicing Donor
misc_feature 2977..3010
/label=lox2272-JT1571
repeat_region 3055..3173
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3055..3162
/label=AAV2 ITR
rep_origin 3248..3703
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3985..4089
/gene="bla"
/label=AmpR promoter
CDS 4090..4950
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5121..5709
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatcttctg gaatcaagcg aaccatcaaa gaaaccgacc ctgattacga
781 ggatgtatct gtggcccttc caaataagcg gcataaagca attgagaatt cagctcgaga
841 tgctgctgtg cagaagattg agactattat caaagaacag tttgctcttg aaatgaagaa
901 taaggaacat gaaattgaag tcattgacca gcgactgatt gaagcaagaa ggatgatgga
961 taaactgcgt gcctgcattg tagcaaacta ctatgcttct gcaggtcttc taaaagtttc
1021 tgagggatca aagacatgtg atacaatggt ttttaatcat cctgctatca agaaattttt
1081 ggaatcacca tctaggtcat catctcctgc caatcagaga gcagaaacac catcagccaa
1141 tcattcagaa agtgattctt tatctcagca caatgacttc ttatctgaca aagataataa
1201 cagcaatatg gatatagagg aaagactctc aaacaacatg gagcagagac caagccgaaa
1261 tactggaagg gatacttcta gaattactgg ctcccataaa acagaacagc ggaatgctga
1321 tctcacagat gagacttcac gactttttgt aaagaaaaca atagtagtgg gcaatgtgtc
1381 caagtatata cctccggata agagggaaga aaatgaccag tcaactcata agtggatggt
1441 atatgtccga gggtcccgta gagaacccag cattaatcat tttgtcaaga aggtttggtt
1501 cttccttcat cctagctata aaccaaatga ccttgtggaa gttagagagc ctccttttca
1561 cctgaccaga agaggctggg gtgagtttcc cgtcagagtt caagttcatt ttaaggacag
1621 ccagaacaag cggatagata tcatacataa tctgaagctg gatagaactt atactggctt
1681 gcagactctt ggagcagaga cggtagtgga tgttgaactc catcgccatt ctctcggaga
1741 agactgtatc tatcctcagt cctcggagtc tgacatctct gatgcccctc catctttgcc
1801 tttgaccatt ccagccccag tgaaagcttc ttcaccaata aagcagtcac atgagccagt
1861 acccgatacc tctgtggaga aaggattccc agctagcact gaagctgaac gacacactcc
1921 gttttatgct ttgccatctt cattggaaag aacacccacc aaaatgacta catcccagaa
1981 agttaccttt tgttcccatg gcaattcagc tttccagcca atagcatcaa gctgcaaaat
2041 tgttccacaa agtcaggttc ctaatcctga gtcacctgga aaatccttcc agcccatcac
2101 catgagctgc aagattgtgt caggttcccc aatatcaact ccaagcccat caccattgcc
2161 tcgaaccccg acttccactc cagtccacgt gaagcaaggc actgccggct ctgttattaa
2221 taatccttat gttatcatgg acaagcagcc ggggcaggtg attggagcca ccactcccag
2281 tacaggaagt cctacaaaca agatctccac ggcttctcag gtctcccaag gaacaggttc
2341 ccctgttcct aaaattcatg gaagtagttt tgtaacatct actgtcaagc aggaggattc
2401 tttgtttgca tctatgccac ctctttgccc aattgggagt caccctaagg ttcaaagccc
2461 caaacctata acaggaggac ttggagcttt cacaaaagtg atcatcaaac aggaacctgg
2521 tgaagcccct cacgtgcccg caacaggagc tgccagccag tcaccactcc cgcagtatgt
2581 gactgtgaaa gggggtcaca tgatagctgt gtcccctcaa aaacaggtca taactcctgg
2641 agaagggatt gcccagtcag caaaggttca gccctccaag gttgtcgggg taccagttgg
2701 gtctgcttta ccttcaacag tgaagcaggc tgtggcgatc agtggtggcc agatcctggt
2761 agccaaggcc agctcttctg tctccaaagc agttgggcca aagcaagttg taacccaagg
2821 agttgccaaa gcaattgtga gtggaggtgg aggaaccatt gttgctcagc cagtgcagac
2881 cttaaccaag gcccaggtta ctgccgctgg tcctcagaag agtggatccc aggtaagtat
2941 caaggttaca agacaggttt aaggagacca ataagctatt attcgtatag gatactttat
3001 acgaagttat taagttatgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
3061 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
3121 gaccaaaggt cgcccggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
3181 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
3241 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
3301 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
3361 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
3421 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
3481 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
3541 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
3601 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
3661 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
3721 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
3781 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
3841 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
3901 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
3961 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
4021 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
4081 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
4141 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
4201 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
4261 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
4321 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
4381 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
4441 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
4501 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
4561 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
4621 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
4681 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
4741 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
4801 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
4861 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
4921 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
4981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
5041 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
5101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
5161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
5221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
5281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
5341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
5401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
5461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
5521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
5581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
5641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
5701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
5761 gctcacatgt
//
