| 质粒名称: | pAAVLINK-EF1a-HA-5'-TNRC6C |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001530 |
| 产品用途: | 5' AAVLINK plasmid for TNRC6C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TNRC6C |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-TNRC6C
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-TNRC6C载体质粒基本信息
pAAVLINK-EF1a-HA-5'-TNRC6C质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-TNRC6C载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-TNRC6C质粒载体简介
pAAVLINK-EF1a-HA-5'-TNRC6C质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-TNRC6C 7264 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7264)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7264
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4426
/codon_start=1
/label=TNRC6C
/translation="EEKKKKKQEEKKKKEGAQKKAADQKTKVPEPTKTCSSQPQPAGTS
TSTSTSTISSSNNGKRASASGQQPAASRYLPREVPPRFRQQEQKQLLKRGQPLPTGTLT
SVSPTQGAGPAGVSPPPLPGAGTQHHPSKLQPDLSHSGLADHYENSHWGQQPTYRSEAN
CSWDKVIIDRTDKEAWPSITGTETESASECTTDTDSASNCGSENSSMATGSAQGNFTGH
TKKTNGNNGTNGALVQSPSNQSALGAGGANSNGSAARVWGVATGSSSGLAHCSVSGGDG
KMDTMIGDGRSQNCWGASNSNAGINLNLNPNANPAAWPVLGHEGTVATGNPSSICSPVS
AIGQNMGNQNGNPTGTLGAWGNLLPQESTEPQTSTSQNVSFSAQPQNLNTDGPNNTNPM
NSSPNPINAMQTNGLPNWGMAVGMGAIIPPHLQGLPGANGSSVSQVSGGSAEGISNSVW
GLSPGNPATGNSNSGFSQGNGDTVNSALSAKQNGSSSAVQKEGSGGNAWDSGPPAGPGI
LAWGRGSGNNGVGNIHSGAWGHPSRSTSNGVNGEWGKPPNQHSNSDINGKGSTGWESPS
VTSQNPTVQPGGEHMNSWAKAASSGTTASEGSSDGSGNHNEGSTGREGTGEGRRRDKGI
IDQGHIQLPRNDLDPRVLSNTGWGQTPVKQNTAWEFEESPRSERKNDNGTEAWGCAATQ
ASNSGGKNDGSIMNSTNTSSVSGWVNAPPAAVPANTGWGDSNNKAPSGPGVWGDSISST
AVSTAAAAKSGHAWSGAANQEDKSPTWGEPPKPKSQHWGDGQRSNPAWSAGGGDWADSS
SVLGHLGDGKKNGSGWDADSNRSGSGWNDTTRSGNSGWGNSTNTKANPGTNWGETLKPG
PQQNWASKPQDNNVSNWGGAASVKQTGTGWIGGPVPVKQKDSSEATGWEEPSPPSIRRK
MEIDDGTSAWGDPSNYNNKTVNMWDRNNPVIQSSTTTNTTTTTTTTTSNTTHRVETPPP
HQAGTQLNRSPLLGPVSSGWGEMPNVHSKTENSWGEPSSPSTLVDNGTAAWGKPPSSGS
GWGDHPAEPPVAFGRAGAPVAASALCKPASKSMQEGWGSGGDEMNLSTSQWEDEEGDVW
NNAASQESTSSCSSWGNAPKKGLQKGMKTSGKQDEAWIMSRLIKQLTDMGFPREPAEEA
LKSNNMNLDQAMSALLEKKVDVDKRGLGVTDHNGMAAKPLGCRPPISKESSVDRPTFLD
K"
misc_feature 1275..1280
/label=SDS
misc_feature 2279..2284
/label=SDS
misc_feature 3066..3071
/label=SDS
misc_feature 3157..3162
/label=SDS
CDS 3655..3672
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
misc_feature 4095..4100
/label=SDS
misc_feature 4098..4103
/label=SDS
misc_feature 4239..4244
/label=SDS
intron 4427..4467
/label=Splicing Donor
misc_feature 4471..4504
/label=lox2272-JT1571
repeat_region 4549..4667
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4549..4656
/label=AAV2 ITR
rep_origin 4742..5197
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5479..5583
/gene="bla"
/label=AmpR promoter
CDS 5584..6444
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6615..7203
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctga agaaaagaaa aagaaaaagc aggaagaaaa
781 gaaaaagaag gaaggtgctc agaaaaaggc tgctgatcaa aaaaccaaag tgccagaacc
841 tactaagacc tgttcaagcc agccccaacc tgccggtacc agtaccagta cttccaccag
901 cactatctca agcagcaaca atggcaaacg tgcatctgcc agtggccagc agccagctgc
961 atcccgttac ctgcctcgtg aggtgcctcc acgcttccgc cagcaagaac agaagcagct
1021 attaaagaga ggccagccat tgcctacggg gactctaacc agtgtgagcc caacccaggg
1081 tgctgggcct gcaggggtaa gcccacctcc cctacctgga gccggaacac agcatcatcc
1141 cagtaagctc caaccagatc tcagtcatag tggattggca gatcattatg aaaattccca
1201 ctggggacag cagcctactt acagaagcga agccaactgc agctgggata aagtgataat
1261 agataggact gacaaggagg cgtggccttc catcacagga acagagactg aatctgcctc
1321 agaatgtact acagacactg actctgcctc caactgtggc tcagagaaca gtagcatggc
1381 tacagggagt gcccagggca acttcactgg acataccaag aagacaaatg gcaataatgg
1441 caccaatggc gcactcgtcc aaagcccttc taatcagagt gcccttggag cagggggagc
1501 gaacagtaat ggaagtgcgg ccagagtgtg gggtgtagcc acaggctcca gctctggtct
1561 ggctcactgc tctgtcagtg gtggggatgg aaaaatggac actatgattg gagatgggag
1621 aagtcagaat tgctggggtg cttccaactc caatgctggc attaatctta accttaatcc
1681 taatgccaac ccagctgcct ggcctgtact tggacatgaa ggaaccgtgg cgacaggcaa
1741 cccttccagt atttgcagtc cagtcagtgc cataggtcaa aatatgggca accagaacgg
1801 gaacccaaca ggcactttag gtgcttgggg aaacttgctg ccacaagaga gcacagaacc
1861 acaaacgtcc acttctcaga atgtgtcttt cagcgcacaa cctcagaacc ttaacactga
1921 tggaccaaat aacactaacc ccatgaactc ttcacccaac cctatcaatg caatgcagac
1981 aaatggactg ccaaactggg gcatggctgt tggtatgggg gccatcatcc cgccccacct
2041 gcaaggcctt cctggtgcta atggatcatc agtttctcaa gtcagtgggg gcagtgctga
2101 aggaataagc aattctgtgt ggggactgtc cccaggtaac cctgccacag gaaatagcaa
2161 ttctgggttc agtcagggga atggagacac tgtgaactca gcattaagtg ctaaacaaaa
2221 tggatccagc agtgctgtgc aaaaggaagg aagtggagga aatgcttggg attcaggacc
2281 tcctgctggt cctggaatac tcgcctgggg aaggggcagt ggcaacaatg gcgttggtaa
2341 tatccattca ggagcttggg gccaccccag ccgaagcacc tctaacggtg tgaatgggga
2401 atggggaaag cccccaaacc agcattccaa cagtgacatc aatgggaaag gatcaacagg
2461 gtgggagagt cctagtgtca ccagccagaa ccctaccgta cagcctggtg gtgaacacat
2521 gaactcctgg gccaaagcgg catcttctgg aactacagca agtgaaggaa gtagtgatgg
2581 ttctggcaac cacaatgaag gaagcactgg gagggaagga acgggagaag gccgaaggcg
2641 agataaaggg attatagacc aagggcacat ccagttgcca aggaatgatc ttgacccaag
2701 agttctgtct aatactggtt ggggacagac tcctgtaaag caaaacactg cctgggaatt
2761 tgaagaatcc cctaggtctg aaaggaaaaa tgacaatggg acagaggcct ggggttgtgc
2821 agctactcag gcttcaaact caggggggaa gaacgatggg tccatcatga acagtacaaa
2881 tacctcttca gtatctgggt gggtcaacgc gccacctgcc gctgtgccag caaacacagg
2941 ttggggagac agcaacaaca aagcgccaag tggcccgggg gtttgggggg actcgataag
3001 ctctactgct gttagtactg ctgctgctgc caagagtggc catgcttgga gtggggccgc
3061 aaatcaggag gacaagtcac ccacctgggg tgagcctcca aagcccaaat cccaacactg
3121 gggagatgga caaagatcaa atccagcctg gagtgcagga gggggagatt gggcagattc
3181 atcgtctgtc cttggacact tgggggatgg gaaaaaaaat ggatctggat gggatgctga
3241 cagtaatagg tcagggtctg gttggaatga caccacgaga tctgggaaca gtggctgggg
3301 caacagcaca aatacaaagg ccaatccagg tacaaactgg ggggagactt taaaacctgg
3361 cccccaacag aactgggcta gcaaacccca agacaacaat gtgagtaact ggggaggagc
3421 tgcttctgtg aaacagacag gaacagggtg gatcgggggg ccggtaccgg tcaaacagaa
3481 ggacagcagt gaagcaactg gctgggaaga accctctcca ccgtccattc gccgcaaaat
3541 ggaaattgat gatggtacct cagcttgggg ggacccaagc aactataaca ataaaactgt
3601 aaacatgtgg gatagaaaca acccggtcat ccagagcagt accacgacca ataccaccac
3661 caccaccacc actaccacga gcaacaccac acacagggtc gagacgccgc ccccgcacca
3721 ggccggtact cagctgaatc gatcaccgtt gcttggtcca gtttcatcag gctggggaga
3781 aatgcctaat gttcactcaa agactgaaaa ctcttgggga gaaccatcct ccccttctac
3841 cctggtggat aatggcacag cagcatgggg gaagccaccc agcagtggca gcgggtgggg
3901 agatcaccct gcagagccgc cggtggcatt tggaagagct ggcgcacctg ttgctgcctc
3961 agccctgtgc aaaccagctt caaaatctat gcaagaaggc tggggcagtg gtggggatga
4021 aatgaacctc agtaccagcc agtgggagga tgaagaaggg gacgtgtgga ataatgctgc
4081 ttcccaagaa agcacctcct cctgcagctc ctgggggaac gcccccaaaa aaggacttca
4141 aaagggcatg aagacgtctg gcaagcagga tgaggcctgg atcatgagcc ggctgatcaa
4201 acaactcaca gacatgggct tcccgagaga gccagctgag gaggccttga agagtaacaa
4261 tatgaatctt gatcaggcca tgagcgctct gctggaaaag aaggtggacg tggacaagcg
4321 tgggctggga gtgaccgacc ataatggaat ggccgccaag cccctcggct gccgcccgcc
4381 aatctccaaa gagtcttccg tggaccgccc cacctttctt gacaaggtaa gtatcaaggt
4441 tacaagacag gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag
4501 ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
4561 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
4621 aggtcgcccg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
4681 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
4741 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
4801 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
4861 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
4921 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
4981 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
5041 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
5101 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
5161 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
5221 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
5281 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
5341 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
5401 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
5461 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
5521 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
5581 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
5641 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
5701 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
5761 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
5821 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
5881 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
5941 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
6001 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
6061 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
6121 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
6181 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
6241 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
6301 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
6361 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
6421 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
6481 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
6541 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
6601 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
6661 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
6721 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
6781 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
6841 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
6901 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
6961 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
7021 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
7081 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
7141 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
7201 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
7261 atgt
//
