| 质粒名称: | pAAVLINK-EF1a-HA-5'-TCF20 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001416 |
| 产品用途: | 5' AAVLINK plasmid for TCF20 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TCF20 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-TCF20载体质粒基本信息
pAAVLINK-EF1a-HA-5'-TCF20质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-TCF20载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-TCF20质粒载体简介
pAAVLINK-EF1a-HA-5'-TCF20质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-TCF20 7203 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7203)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7203
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4365
/codon_start=1
/label=TCF20
/translation="QSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGG
SSGSSGSGSGGGRRGAAAAAAAMASETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQ
PPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQAQHSGLGGVSHYQQDYTGPFSPGS
AQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQPMQRPSTLPSSAAG
YQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSNAQ
AYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQ
SQVGQYNQPEVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQ
GSVPMGSRNRILQLMPQLSPTPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSAL
STQVANLPNTVQHMLLSDALTPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAES
LDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAA
REEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQ
RPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSR
TEPSKSPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQ
EVLQGYHHHPDRRYSRSTQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLE
NPHWGPWERKSSSTAPEMKQINLTDYPIPRKFEIEPQSSAHEPGGSLSERRSVICDISP
LRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVILPGGLVSMETKLKSQSGQIKEE
DFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGPQDSRPTPMRR
VPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLH
TPFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGV
IAAAQHRQEGPRKSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSS
DGLPNKGMELKHGSQKLQESCWDLSRQTSPAKSSGPPGMSSQK"
misc_feature 789..794
/label=SDS
misc_feature 1049..1054
/label=SDS
misc_feature 1452..1457
/label=SDS
misc_feature 1455..1460
/label=SDS
misc_feature 1458..1463
/label=SDS
misc_feature 1461..1466
/label=SDS
misc_feature 1464..1469
/label=SDS
misc_feature 1467..1472
/label=SDS
misc_feature 2707..2712
/label=SDS
misc_feature 2710..2715
/label=SDS
misc_feature 2713..2718
/label=SDS
misc_feature 2986..2991
/label=SDS
misc_feature 3590..3595
/label=SDS
misc_feature 4080..4085
/label=SDS
misc_feature 4206..4211
/label=SDS
intron 4366..4406
/label=Splicing Donor
misc_feature 4410..4443
/label=lox2272-JT1571
repeat_region 4488..4606
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4488..4595
/label=AAV2 ITR
rep_origin 4681..5136
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5418..5522
/gene="bla"
/label=AmpR promoter
CDS 5523..6383
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6554..7142
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctcagt cctttcggga gcaaagcagt taccacggaa accagcaaag
781 ctacccacag gaggtacacg gctcatcccg gctagaagag ttcagccctc gtcaggccca
841 gatgttccag aattttggag gtacaggtgg cagtagtggc agcagtggca gtggcagtgg
901 tggtggacga cgaggagcag cagctgctgc ggcagcgatg gctagcgaga cctctggcca
961 tcaaggttac cagggtttca ggaaagaggc tggagatttt tactacatgg caggcaacaa
1021 agaccccgtg actacaggaa ccccacagcc tcctcagcga aggccttctg ggcctgtgca
1081 gagctatgga cccccccagg ggagcagctt tggcaatcag tatgggagtg agggtcatgt
1141 gggccagttt caagcacagc actctggcct tggcggtgtg tcacattatc agcaggatta
1201 cactgggcct ttctctccag ggagtgctca gtaccaacag caggcttcca gccagcagca
1261 gcagcagcaa gtccagcagt tgagacaaca gctttaccag tcccatcagc ccctgccaca
1321 ggccactggc caaccagcat ccagctcatc ccatctacag ccaatgcagc ggccctcaac
1381 tctgccatcc tctgctgctg gttaccagtt aagagtgggt cagtttggcc aacactatca
1441 gtcttctgct tcctcctcct cctcctcctc cttcccttca ccacagcgtt ttagccagtc
1501 tggacagagc tatgatggca gttacaatgt gaatgctgga tctcagtatg aaggacacaa
1561 tgtgggttct aatgcacagg cttatggaac acaatccaat tacagctatc agcctcaatc
1621 tatgaagaat tttgaacagg caaagattcc acaagggacc caacaggggc agcagcagca
1681 gcaaccgcag caacaacaac acccttctca gcatgtgatg cagtatacta acgctgccac
1741 caagctgccc ctgcaaagcc aagtggggca gtacaaccag cctgaggttc ctgtgaggtc
1801 ccccatgcag tttcaccaga acttcagccc catttctaac ccttctccag ctgcctctgt
1861 ggttcagtct ccaagctgta gttctacccc atctcctctc atgcagactg gggagaatct
1921 ccagtgtggg caaggcagtg tgcctatggg ttccagaaac agaattttac agttaatgcc
1981 tcaactcagt ccaaccccat caatgatgcc cagtcctaat tctcatgctg caggcttcaa
2041 agggtttgga ctagaagggg taccagaaaa gcgactgaca gatcctgggt tgagtagttt
2101 gagtgctctg agtactcaag tggccaatct tcctaacact gtccagcaca tgttactttc
2161 tgatgccctg actcctcaga agaagacctc caagaggccc tcatcttcca agaaagcaga
2221 tagctgcaca aattctgaag gctcctcaca acctgaagaa cagctgaagt cccctatggc
2281 agagtcatta gatggaggct gctccagcag ttcagaggat caaggcgaga gagtgcggca
2341 actaagtggc cagagcacca gctctgacac cacctacaag ggtggagcct ctgagaaagc
2401 tggctcctca ccggcacaag gtgctcagaa tgaacccccc agactcaatg ctagtcctgc
2461 cgcaagagaa gaggccacct caccaggcgc taaggacatg ccattgtcat ccgacgggaa
2521 cccaaaggtt aatgagaaga ctgttggggt gattgtctcc cgggaagcca tgacaggtcg
2581 ggtagaaaag cctggtggac aagataaagg ctcccaagag gatgatcctg cagccactca
2641 aaggccacct agcaatggtg gggcaaagga aaccagtcat gcatcacttc cccagccaga
2701 gcctccagga ggaggaggga gcaaaggaaa caagaatggc gataacaact ccaaccataa
2761 tggagaagga aatggccaga gtggccactc tgcagcgggc cctggtttta cgagcagaac
2821 tgagcctagc aaatctcctg gaagtctgcg ctatagttac aaagatagtt tcgggtcagc
2881 cgtgccacga aatgtcagtg gctttcctca gtatcctaca gggcaagaaa agggagattt
2941 cactggccat ggggaacgaa agggtagaaa tgaaaaattc ccaagcctcc tgcaggaagt
3001 gcttcagggt taccaccacc accctgacag gagatattct aggagtactc aagagcatca
3061 ggggatggct ggtagcctag aaggaaccac aaggcccaat gtcttggtta gtcaaaccaa
3121 tgaattagct agcaggggcc ttctgaacaa aagcattggg tctctattag aaaatcccca
3181 ctggggcccc tgggaaagga aatcaagcag cacagctcct gaaatgaaac agatcaattt
3241 gactgactat ccaattccca gaaagtttga aatagagcct cagtcatcag cacatgagcc
3301 tgggggttcc ctctctgaaa gaagatcagt gatctgtgat atttctccac taagacagat
3361 tgtcagggac ccaggggctc actcactggg acacatgagt gccgacacca gaattgggag
3421 gaatgaccgt ctcaatccaa ctttaagtca gtcggtcatt cttcctggtg gtttggtgtc
3481 catggaaacc aagctgaaat cccagagcgg gcagataaaa gaggaagact ttgaacagtc
3541 taaatctcaa gctagtttca acaacaagaa atctggagac cactgccatc ctcctagcat
3601 caagcatgag tcttaccgcg gcaatgccag ccctggagca gcaacccatg attccctttc
3661 agactatggc ccgcaagaca gcagacccac gccaatgcgg cgggtccctg gcagagttgg
3721 tggtcgggag ggcatgaggg gtcggtcccc ttctcaatat catgactttg cagaaaaatt
3781 gaaaatgtct cctgggcgga gcagaggccc agggggagac cctcatcaca tgaatccaca
3841 catgaccttt tcagagaggg ctaaccggag ttctttacac actccctttt ctcccaactc
3901 agaaaccctg gcctctgctt atcatgcaaa tactcgggct catgcttatg gggaccctaa
3961 cgcaggtttg aattctcagc tgcattataa gagacagatg taccaacagc aaccagagga
4021 gtataaagac tggagcagcg gttctgctca gggagtaatt gctgcagcac agcacaggca
4081 ggaggggcca cggaagagtc caaggcagca gcagtttctt gacagagtac ggagccctct
4141 gaaaaatgac aaagatggta tgatgtatgg cccaccagtg gggacttacc atgaccccag
4201 tgcccaggag gctgggcgct gcctaatgtc tagtgatggt ctgcctaaca agggcatgga
4261 attaaagcat ggctcccaga agttacaaga atcctgttgg gatctttctc ggcaaacttc
4321 tccagccaaa agcagcggtc ctccaggaat gtccagtcaa aaaaggtaag tatcaaggtt
4381 acaagacagg tttaaggaga ccaataagct attattcgta taggatactt tatacgaagt
4441 tattaagtta tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt
4501 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
4561 ggtcgcccgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
4621 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
4681 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4741 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4801 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4861 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
4921 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
4981 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
5041 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
5101 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
5161 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
5221 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
5281 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
5341 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
5401 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
5461 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
5521 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
5581 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
5641 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
5701 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
5761 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
5821 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
5881 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
5941 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
6001 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
6061 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
6121 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
6181 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
6241 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
6301 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
6361 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
6421 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
6481 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
6541 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
6601 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
6661 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
6721 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
6781 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
6841 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
6901 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
6961 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
7021 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
7081 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
7141 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
7201 tgt
//
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pAAVLINK-EF1a-HA-5'-TCF20质粒载体应用举例
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