| 质粒名称: | pAAVLINK-EF1a-HA-5'-SMARCA4 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001392 |
| 产品用途: | 5' AAVLINK plasmid for SMARCA4 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SMARCA4 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-SMARCA4载体质粒基本信息
pAAVLINK-EF1a-HA-5'-SMARCA4质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-SMARCA4载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-SMARCA4质粒载体简介
pAAVLINK-EF1a-HA-5'-SMARCA4质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-SMARCA4 6647 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6647)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6647
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3787
/codon_start=1
/label=SMARCA4
/translation="STPDPPLGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHSMMGPS
PGPPSAGHPIPTQGPGGYPQDNMHQMHKPMESMHEKGMSDDPRYNQMKGMGMRSGGHAG
MGPPPSPMDQHSQGYPSPLGGSEHASSPVPASGPSSGPQMSSGPGGAPLDGADPQALGQ
QNRGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQQMPTLPPP
SVSATGPGPGPGPGPGPGPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGPPK
PWPEGPMANAAAPTSTPQKLIPPQPTGRPSPAPPAVPPAASPVMPPQTQSPGQPAQPAP
MVPLHQKQSRITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKA
TIELKALRLLNFQRQLRQEVVVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQ
QKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVTGKIQKLTKAVATYHANTEREQKKE
NERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTELVRQHKAAQVA
KEKKKKKKKKKAENAEGQTPAIGPDGEPLDETSQMSDLPVKVIHVESGKILTGTDAPKA
GQLEAWLEMNPGYEVAPRSDSEESGSEEEEEEEEEEQPQAAQPPTLPVEEKKKIPDPDS
DDVSEVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVL
KQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLS
TLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAK
IRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPT
IFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPE
KVEYVIKCDMSALQRVLYRHMQAK"
misc_feature 1192..1197
/label=SDS
misc_feature 1571..1576
/label=SDS
misc_feature 1998..2003
/label=SDS
misc_feature 2349..2354
/label=SDS
misc_feature 2745..2750
/label=SDS
misc_feature 2748..2753
/label=SDS
misc_feature 2751..2756
/label=SDS
misc_feature 3618..3623
/label=SDS
intron 3788..3828
/label=Splicing Donor
misc_feature 3832..3865
/label=lox2272-JT1571
repeat_region 3910..4050
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3910..4039
/label=AAV2 ITR
rep_origin 4125..4580
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4862..4966
/gene="bla"
/label=AmpR promoter
CDS 4967..5827
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5998..6586
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatcttc cactccagac ccacccctgg gcggaactcc
781 tcggccaggt ccttccccgg gccctggccc ttcccctgga gccatgctgg gccctagccc
841 gggtccctcg ccgggctccg cccacagcat gatggggccc agcccagggc cgccctcagc
901 aggacacccc atccccaccc aggggcctgg agggtaccct caggacaaca tgcaccagat
961 gcacaagccc atggagtcca tgcatgagaa gggcatgtcg gacgacccgc gctacaacca
1021 gatgaaagga atggggatgc ggtcaggggg ccatgctggg atggggcccc cgcccagccc
1081 catggaccag cactcccaag gttacccctc gcccctgggt ggctctgagc atgcctctag
1141 tccagttcca gccagtggcc cgtcttcggg gccccagatg tcttccgggc caggaggtgc
1201 cccgctggat ggtgctgacc cccaggcctt ggggcagcag aaccggggcc caaccccatt
1261 taaccagaac cagctgcacc agctcagagc tcagatcatg gcctacaaga tgctggccag
1321 ggggcagccc ctccccgacc acctgcagat ggcggtgcag ggcaagcggc cgatgcccgg
1381 gatgcagcag cagatgccaa cgctacctcc accctcggtg tccgcaacag gacccggccc
1441 tggccctggc cctggccccg gcccgggtcc cggcccggca cctccaaatt acagcaggcc
1501 tcatggtatg ggagggccca acatgcctcc cccaggaccc tcgggcgtgc cccccgggat
1561 gccaggccag cctcctggag ggcctcccaa gccctggcct gaaggaccca tggcgaatgc
1621 tgctgccccc acgagcaccc ctcagaagct gattcccccg cagccaacgg gccgcccttc
1681 ccccgcgccc cctgccgtcc cacccgccgc ctcgcccgtg atgccaccgc agacccagtc
1741 ccccgggcag ccggcccagc ccgcgcccat ggtgccactg caccagaagc agagccgcat
1801 cacccccatc cagaagccgc ggggcctcga ccctgtggag atcctgcagg agcgcgagta
1861 caggctgcag gctcgcatcg cacaccgaat tcaggaactt gaaaaccttc ccgggtccct
1921 ggccggggat ttgcgaacca aagcgaccat tgagctcaag gccctcaggc tgctgaactt
1981 ccagaggcag ctgcgccagg aggtggtggt gtgcatgcgg agggacacag cgctggagac
2041 agccctcaat gctaaggcct acaagcgcag caagcgccag tccctgcgcg aggcccgcat
2101 cactgagaag ctggagaagc agcagaagat cgagcaggag cgcaagcgcc ggcagaagca
2161 ccaggaatac ctcaatagca ttctccagca tgccaaggat ttcaaggaat atcacagatc
2221 cgtcacaggc aaaatccaga agctgaccaa ggcagtggcc acgtaccatg ccaacacgga
2281 gcgggagcag aagaaagaga acgagcggat cgagaaggag cgcatgcgga ggctcatggc
2341 tgaagatgag gaggggtacc gcaagctcat cgaccagaag aaggacaagc gcctggccta
2401 cctcttgcag cagacagacg agtacgtggc taacctcacg gagctggtgc ggcagcacaa
2461 ggctgcccag gtcgccaagg agaaaaagaa gaaaaagaaa aagaagaagg cagaaaatgc
2521 agaaggacag acgcctgcca ttgggccgga tggcgagcct ctggacgaga ccagccagat
2581 gagcgacctc ccggtgaagg tgatccacgt ggagagtggg aagatcctca caggcacaga
2641 tgcccccaaa gccgggcagc tggaggcctg gctcgagatg aacccggggt atgaagtagc
2701 tccgaggtct gatagtgaag aaagtggctc agaagaagag gaagaggagg aggaggaaga
2761 gcagccgcag gcagcacagc ctcccaccct gcccgtggag gagaagaaga agattccaga
2821 tccagacagc gatgacgtct ctgaggtgga cgcgcggcac atcattgaga atgccaagca
2881 agatgtcgat gatgaatatg gcgtgtccca ggcccttgca cgtggcctgc agtcctacta
2941 tgccgtggcc catgctgtca ctgagagagt ggacaagcag tcagcgctta tggtcaatgg
3001 tgtcctcaaa cagtaccaga tcaaaggttt ggagtggctg gtgtccctgt acaacaacaa
3061 cctgaacggc atcctggccg acgagatggg cctggggaag accatccaga ccatcgcgct
3121 catcacgtac ctcatggagc acaaacgcat caatgggccc ttcctcatca tcgtgcctct
3181 ctcaacgctg tccaactggg cgtacgagtt tgacaagtgg gccccctccg tggtgaaggt
3241 gtcttacaag ggatccccag cagcaagacg ggcctttgtc ccccagctcc ggagtgggaa
3301 gttcaacgtc ttgctgacga cgtacgagta catcatcaaa gacaagcaca tcctcgccaa
3361 gatccgttgg aagtacatga ttgtggacga aggtcaccgc atgaagaacc accactgcaa
3421 gctgacgcag gtgctcaaca cgcactatgt ggcaccccgc cgcctgctgc tgacgggcac
3481 accgctgcag aacaagcttc ccgagctctg ggcgctgctc aacttcctgc tgcccaccat
3541 cttcaagagc tgcagcacct tcgagcagtg gtttaacgca ccctttgcca tgaccgggga
3601 aaaggtggac ctgaatgagg aggaaaccat tctcatcatc cggcgtctcc acaaagtgct
3661 gcggcccttc ttgctccgac gactcaagaa ggaagtcgag gcccagttgc ccgaaaaggt
3721 ggagtacgtc atcaagtgcg acatgtctgc gctgcagcga gtgctctacc gccacatgca
3781 ggccaaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
3841 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
3901 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
3961 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
4021 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
4081 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4141 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4201 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4261 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4321 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4381 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
4441 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
4501 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
4561 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
4621 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
4681 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
4741 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
4801 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
4861 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
4921 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
4981 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
5041 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
5101 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5161 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5221 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5281 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5341 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
5401 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
5461 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
5521 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
5581 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
5641 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
5701 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
5761 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
5821 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
5881 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
5941 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
6001 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
6061 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6121 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6181 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6241 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6301 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6361 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
6421 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
6481 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6541 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
6601 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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pAAVLINK-EF1a-HA-5'-SMARCA4质粒载体应用举例
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