| 质粒名称: | pAAVLINK-EF1a-HA-5'-SHANK2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001410 |
| 产品用途: | 5' AAVLINK plasmid for SHANK2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SHANK2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-SHANK2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-SHANK2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-SHANK2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-SHANK2质粒载体简介
pAAVLINK-EF1a-HA-5'-SHANK2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-SHANK2 6755 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6755)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6755
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3895
/codon_start=1
/label=SHANK2
/translation="MPRSPTSSEDEMAQSFSDYSVGSESDSSKEETIYDTIRATAEKPG
GARTEESQGNTLVIRVVIHDLQQTKCIRFNPDATVWVAKQRILCTLTQSLKDVLNYGLF
QPASNGRDGKFLDEERLLREYPQPVGEGVPSLEFRYKKRVYKQASLDEKQLAKLHTKTN
LKKCMDHIQHRLVEKITKMLDRGLDPNFHDPETGETPLTLAAQLDDSVEVIKALKNGGA
HLDFRAKDGMTALHKAARARNQVALKTLLELGASPDYKDSYGLTPLYHTAIVGGDPYCC
ELLLHEHATVCCKDENGWHEIHQACRYGHVQHLEHLLFYGADMSAQNASGNTALHICAL
YNQDSCARVLLFRGGNKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDIVPFREAPA
YSNRRRRPPNTLAAPRVLLRSNSDNNLNASAPDWAVCSTATSHRSLSPQLLQQMPSKPE
GAAKTIGSYVPGPRSRSPSLNRLGGAGEDGKRPQPLWHVGSPFALGANKDSLSAFEYPG
PKRKLYSAVPGRLFVAVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFP
AECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDTSSDCIIEEKTVVLQKKDNE
GFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVV
KVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMT
SELEELVDKASVRKKKDKPEEIVPASKPSRAAENMAVEPRVATIKQRPSSRCFPAGSDM
NSVYERQGIAVMTPTVPGSPKAPFLGIPRGTMRRQKSIDSRIFLSGITEEERQFLAPPM
LKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPKSPTPRVYGTIKPAFNQNSAA
KVSPATRSDTVATMMREKGMYFRRELDRYSLDSEDLYSRNAGPQANFRNKRGQMPENPY
SEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSS
Q"
misc_feature 2490..2495
/label=SDS
intron 3896..3936
/label=Splicing Donor
misc_feature 3940..3973
/label=lox2272-JT1571
repeat_region 4018..4158
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4018..4147
/label=AAV2 ITR
rep_origin 4233..4688
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4970..5074
/gene="bla"
/label=AmpR promoter
CDS 5075..5935
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6106..6694
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gccgcgcagc ccaacatcca gcgaggacga
781 gatggcccag agcttctccg actactccgt ggggtcggag tcagacagct ccaaagaaga
841 gaccatctat gacacgatcc gggccactgc ggagaagccg ggcggtgcca ggacggagga
901 gagccagggc aacacgctgg tgatccgcgt ggtcatccat gacctgcagc agacgaaatg
961 cattcgattt aacccggatg ccacagtgtg ggttgcaaag cagcggatcc tgtgtacatt
1021 aacccagagt ttgaaagatg tcctgaacta cggcctgttc cagccggcca gcaatgggcg
1081 tgacggcaag ttcctggatg aggagcggct cctgcgcgag tacccacagc ccgtgggtga
1141 gggcgttcct tccctggagt ttcgatacaa gaagcgggtg tataaacaag ccagtctcga
1201 tgagaaacag ttggccaagc tccacacgaa gaccaatctg aagaaatgca tggatcacat
1261 tcagcatcgc ttggtggaga agatcaccaa gatgctggac cgaggcctgg atcccaattt
1321 ccacgacccg gagaccggag agacccccct gaccttagcc gctcagctgg acgactctgt
1381 ggaggtcatc aaagctctca aaaatggtgg agctcacctg gacttccgtg ccaaagatgg
1441 gatgaccgcc ctacacaaag ctgcccgagc gaggaaccaa gttgccctga agaccctttt
1501 agagcttggt gcatccccag attataaaga cagttacggc ctcaccccgc tgtatcacac
1561 agccatcgtc ggaggtgatc cctactgctg cgagcttctc ctgcacgaac acgccactgt
1621 gtgctgcaaa gatgagaacg gctggcacga gatccaccag gcctgcaggt acgggcacgt
1681 gcagcacctg gagcacctgc tgttctacgg ggcagacatg agtgcccaga atgcctcggg
1741 gaacacggcc ttgcacatct gcgccctcta caaccaggac agctgtgcaa gagtgcttct
1801 gtttcgaggc ggaaataagg agttaaaaaa ctacaacagc cagactccat ttcaggtggc
1861 cataatagca ggcaactttg agctggcaga atacatcaag aaccacaagg aaacagacat
1921 tgtgcccttc cgagaggccc cggcgtactc caaccgccgg cggcggcccc ccaacacgct
1981 ggccgccccc cgggtcctgc tgcgctccaa cagtgacaac aacctcaatg ccagcgctcc
2041 cgactgggcc gtctgctcca cggccacctc gcaccgcagc ctgtcacccc agctgctgca
2101 gcagatgccc agcaagcccg agggggccgc gaagaccatt gggagctacg tgcccgggcc
2161 ccgcagccgg tccccatcgc tcaacaggct gggcggcgca ggcgaggacg gcaagaggcc
2221 gcagcctctc tggcatgtcg ggtcgccttt tgctcttggt gccaacaagg actcactctc
2281 ggccttcgag tacccggggc ccaagcggaa gctctacagt gccgtgcccg ggaggctctt
2341 cgtcgctgtc aagccatacc aaccccaagt ggacggcgag atcccccttc accgcggtga
2401 cagggtcaaa gttctgagca tcggtgaagg gggcttctgg gaaggcagcg cccgcggcca
2461 catcggatgg tttccggcgg agtgcgtgga ggaggtccag tgtaagccca gggacagcca
2521 ggcagaaacg cgcgctgacc gcagcaagaa gcttttcagg cactacaccg tgggctccta
2581 tgacagcttc gacacttcca gtgactgcat tattgaggag aagacggtgg tcctgcagaa
2641 aaaagacaat gagggctttg gattcgtgct tcgaggggcc aaagctgaca cacccattga
2701 agaattcaca ccaacaccgg ctttcccagc cctacagtac ctggagtccg tggatgaagg
2761 tggggtggcg tggcaagccg gactaaggac cggggacttc ttgattgagg ttaacaatga
2821 gaatgttgtc aaagtcggcc acaggcaggt ggtgaacatg atccggcagg gagggaatca
2881 cctggtcctt aaggtggtca cggtgaccag gaatctggac cccgacgaca ccgccaggaa
2941 gaaagctccc ccgcctccaa agcgggcacc gaccacagcc ctcaccctgc gctccaagtc
3001 catgacctcg gagctggagg agctcgtgga taaagcctcg gtccggaaga agaaggataa
3061 acccgaggag atagtcccgg cctccaagcc ctcccgcgct gctgagaaca tggctgtgga
3121 accgagggtg gcgaccatca agcagcggcc cagcagccgg tgcttcccgg cgggctcaga
3181 catgaactct gtgtacgaac gccaaggaat cgccgtgatg acgcccactg ttcctgggag
3241 cccaaaagcc ccgtttctgg gcatccctcg aggtacgatg cgaaggcaga aatcaataga
3301 cagcagaatc tttctatcag gaataacaga ggaagagcgg cagtttctgg ctcctccaat
3361 gctgaagttc accagaagcc tgtccatgcc ggacacctct gaggacatcc cccctccacc
3421 gcagtctgtg cccccgtccc caccaccacc ttccccaacc acttacaact gccccaagtc
3481 cccaactcca agagtctacg ggacgattaa gcctgcgttc aatcagaatt ctgccgccaa
3541 ggtgtccccc gccaccaggt ccgacaccgt ggccaccatg atgagggaga aggggatgta
3601 cttcaggaga gagctggacc gctactcctt ggactctgaa gacctctaca gtcggaatgc
3661 cggcccgcaa gccaacttcc gcaacaagag aggccagatg ccagaaaacc catactcaga
3721 ggtggggaag atcgccagca aagccgtcta cgtccccgcc aagcccgcca ggcggaaggg
3781 gatgctggtg aagcagtcca acgtggagga cagccccgag aagacgtgct ccatccctat
3841 cccgaccatc atcgtgaagg agccgtccac cagcagcagc ggcaagagca gccaggtaag
3901 tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta taggatactt
3961 tatacgaagt tattaagtta tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
4021 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
4081 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
4141 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
4201 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
4261 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
4321 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
4381 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4441 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4501 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4561 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
4621 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
4681 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
4741 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
4801 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
4861 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
4921 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
4981 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
5041 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
5101 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
5161 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
5221 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
5281 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
5341 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
5401 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
5461 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
5521 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
5581 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
5641 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
5701 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
5761 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
5821 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
5881 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
5941 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
6001 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
6061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
6121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
6181 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
6241 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
6301 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
6361 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
6421 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
6481 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
6541 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
6601 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
6661 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
6721 gttcctggcc ttttgctggc cttttgctca catgt
//
