| 质粒名称: | pAAVLINK-EF1a-HA-5'-SETD1A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001395 |
| 产品用途: | 5' AAVLINK plasmid for SETD1A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETD1A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-SETD1A载体质粒基本信息
pAAVLINK-EF1a-HA-5'-SETD1A质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-SETD1A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-SETD1A质粒载体简介
pAAVLINK-EF1a-HA-5'-SETD1A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-SETD1A 6901 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6901)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6901
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4041
/codon_start=1
/label=SETD1A
/translation="DQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVH
FSVNDSKYIPVEDLQDPRCHVRSKNRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNV
RETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTSVMGNI
IHAQLDIKGQQRMKYYELIVNGSYTPQTVPTGGKALSEKFQGSGAATETAESRRRSSSD
TAAYPAGTTAVGTPGNGTPCSQDTSFSSSRQDTPSSFGQFTPQSSQGTPYTSRGSTPYS
QDSAYSSSTTSTSFKPRRSENSYQDAFSRRHFSASSASTTASTAIAATTAATASSSASS
SSLSSSSSSSSSSSSSQFRSSDANYPAYYESWNRYQRHTSYPPRRATREEPPGAPFAEN
TAERFPPSYTSYLPPEPSRPTDQDYRPPASEAPPPEPPEPGGGGGGGGPSPEREEVRTS
PRPASPARSGSPAPETTNESVPFAQHSSLDSRIEMLLKEQRSKFSFLASDTEEEEENSS
MVLGARDTGSEVPSGSGHGPCTPPPAPANFEDVAPTGSGEPGATRESPKANGQNQASPC
SSGDDMEISDDDRGGSPPPAPTPPQQPPPPPPPPPPPPPYLASLPLGYPPHQPAYLLPP
RPDGPPPPEYPPPPPPPPHIYDFVNSLELMDRLGAQWGGMPMSFQMQTQMLTRLHQLRQ
GKGLIAASAGPPGGAFGEAFLPFPPPQEAAYGLPYALYAQGQEGRGAYSREAYHLPMPM
AAEPLPSSSVSGEEARLPPREEAELAEGKTLPTAGTVGRVLAMLVQEMKSIMQRDLNRK
MVENVAFGAFDQWWESKEEKAKPFQNAAKQQAKEEDKEKTKLKEPGLLSLVDWAKSGGT
TGIEAFAFGSGLRGALRLPSFKVKRKEPSEISEASEEKRPRPSTPAEEDEDDPEQEKEA
GEPGRPGTKPPKRDEERGKTQGKHRKSFALDSEGEEASQESSSEKDEEDDEEDEEDEDR
EEAVDTTKKETEVSDGEDEESDSSSKCSLYADSDGENDSTSDSESSSSSSSSSSSSSSS
SSSSSSSSSESSSEDEEEEERPAALPSASPPPREVPVPTPAPVEVPVPE"
misc_feature 1120..1125
/label=SDS
misc_feature 1626..1631
/label=SDS
misc_feature 1767..1772
/label=SDS
misc_feature 1785..1790
/label=SDS
misc_feature 1803..1808
/label=SDS
misc_feature 1974..1979
/label=SDS
misc_feature 2024..2029
/label=SDS
misc_feature 2083..2088
/label=SDS
misc_feature 2277..2282
/label=SDS
misc_feature 2280..2285
/label=SDS
misc_feature 2582..2587
/label=SDS
misc_feature 2585..2590
/label=SDS
misc_feature 2641..2646
/label=SDS
misc_feature 2690..2695
/label=SDS
misc_feature 2910..2915
/label=SDS
misc_feature 2955..2960
/label=SDS
misc_feature 3024..3029
/label=SDS
misc_feature 3027..3032
/label=SDS
misc_feature 3045..3050
/label=SDS
misc_feature 3234..3239
/label=SDS
misc_feature 3282..3287
/label=SDS
misc_feature 3285..3290
/label=SDS
misc_feature 3531..3536
/label=SDS
misc_feature 3642..3647
/label=SDS
misc_feature 3660..3665
/label=SDS
misc_feature 3678..3683
/label=SDS
misc_feature 3873..3878
/label=SDS
misc_feature 3876..3881
/label=SDS
misc_feature 3879..3884
/label=SDS
misc_feature 3882..3887
/label=SDS
misc_feature 3897..3902
/label=SDS
misc_feature 3924..3929
/label=SDS
intron 4042..4082
/label=Splicing Donor
misc_feature 4086..4119
/label=lox2272-JT1571
repeat_region 4164..4304
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4164..4293
/label=AAV2 ITR
rep_origin 4379..4834
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5116..5220
/gene="bla"
/label=AmpR promoter
CDS 5221..6081
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6252..6840
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctga tcaggaaggt gggggagatg ggcagaaggc
781 cccgagcttc cagtggcgga actacaagct catcgtggat cctgccttgg accctgccct
841 gcgcaggcct tctcagaagg tgtaccgcta tgatggagtc cacttcagtg tcaacgactc
901 aaagtatata ccagtcgaag acctccaaga cccccgttgc catgtcaggt ccaaaaacag
961 agacttttcc ctcccagtcc ctaagtttaa gctggacgag ttctatattg gacagattcc
1021 actgaaggaa gtgacttttg caaggctgaa tgacaacgtg cgggagacct tcctgaagga
1081 tatgtgccgt aagtacggtg aggtggaaga ggtagagatc ctccttcacc cccgtacgcg
1141 caagcacctg ggcctggccc gtgtgctctt caccagcact cggggcgcca aggaaacggt
1201 caaaaacctc caccttacct ccgtcatggg caacatcatc catgcccagc ttgacatcaa
1261 aggacaacaa cgaatgaaat actatgaact aattgtcaat ggctcctaca cccctcagac
1321 tgtgcccact gggggcaagg ccctgagtga gaagttccaa ggctcgggtg cagccactga
1381 gacggccgaa tcccgccgcc gctcttcctc tgacacagct gcctacccag caggcaccac
1441 tgcggtgggc actcctggca acggcacccc ctgctcccag gacacaagct tctccagcag
1501 ccgacaagat accccatctt cctttggcca gttcacacct cagtcctccc aaggaacccc
1561 ctacacgtct cggggcagca ccccctactc tcaggactct gcctactcca gcagcaccac
1621 ttcaacctcc ttcaagcccc ggcggtcaga gaacagctac caagatgcct tttcccgccg
1681 ccacttctct gcatcttcag cctccacaac cgcctccacg gccatcgccg ccaccactgc
1741 agccactgcc tcatcctccg cctcttcctc ctcattgtcc tcgtcctcct cgtcatcctc
1801 ttcctcctcg tcctctcagt ttcgtagttc tgatgcaaac tacccagcgt attatgaaag
1861 ctggaatcgc taccagcgcc atacttccta cccaccacgc cgggccacac gggaggaacc
1921 ccctggagcc ccttttgctg aaaatacagc tgagcgcttc ccaccttctt acacctccta
1981 cctgcccccc gagcccagcc ggcccaccga ccaggactac cggcctcctg cctcagaggc
2041 tccacccccg gagcctccag aacctggtgg aggcgggggt ggaggagggc ccagccctga
2101 gagagaagaa gttcggactt ccccccgccc agcctcccct gcccgctctg gctccccagc
2161 cccggagacc accaatgaga gtgtgccctt cgcccagcac agcagcctgg attcccgcat
2221 cgagatgctg ctgaaggagc agcgctccaa gttttccttc ttggcctctg acacagagga
2281 ggaggaagag aacagcagca tggtccttgg ggccagagat acagggagtg aggtgccttc
2341 tgggtcaggg catgggccct gcacaccccc tccggcccca gctaattttg aggatgtggc
2401 acctacaggg agcggggagc caggggctac ccgggagtct cccaaggcaa atggacagaa
2461 ccaggcttct ccatgctctt ctggagacga catggagatc tccgacgacg accggggtgg
2521 ctcaccccct ccggccccga cgccccctca gcagcctccg ccacctcccc ctcccccgcc
2581 gcctcctcct ccctacctgg cgtcccttcc tcttggttat cctccccacc aacctgccta
2641 cctcctccca cccagacctg atgggccgcc gccccctgag taccccccac ctcctccacc
2701 acccccgcac atctatgact ttgtgaactc cttggagctc atggaccgac ttggggctca
2761 gtggggaggg atgcccatgt ccttccagat gcagacccag atgttaactc ggctccatca
2821 gctgcggcag ggcaagggat tgattgccgc ctcagctggc ccccccggtg gggcctttgg
2881 ggaggccttc ctcccgtttc cacccccgca ggaggcagcc tacggcttgc cgtatgctct
2941 atatgcacag gggcaggagg gcagaggggc atactcacgg gaggcctacc acctgcccat
3001 gccaatggca gccgagcccc tgccctcctc ctcagtctcg ggagaggagg cccggctgcc
3061 acccagggaa gaagcagagc tggcagaggg caagaccctc ccgacagcag gcaccgtggg
3121 ccgtgtgctc gccatgctgg tccaggagat gaagagcatc atgcagcgag acctcaaccg
3181 caagatggtg gagaacgtgg ccttcggagc ctttgaccag tggtgggaga gcaaggagga
3241 gaaggccaag ccattccaga acgcggccaa gcagcaagcc aaggaggagg ataaagagaa
3301 gacgaagctg aaggagcctg gcctgctgtc cctcgtggac tgggccaaga gcgggggcac
3361 tacgggcatc gaggctttcg cctttgggtc agggctgaga ggggccctgc ggctgccttc
3421 attcaaggta aagcggaaag agccatcgga aatttccgag gccagtgagg aaaagaggcc
3481 tcgtccctcc actcctgctg aggaagatga agacgaccct gaacaagaga aggaggctgg
3541 agagccagga cgtccgggga ccaagccccc gaagcgggac gaagagcgag gcaagaccca
3601 gggcaagcac cgcaagtcct ttgctctgga cagcgaaggg gaggaggcat cccaggagtc
3661 ctcctcggag aaggatgagg aggatgacga ggaagatgag gaagatgaag atcgagagga
3721 agctgtggat accacaaaga aggagacaga ggtgtcggat ggcgaggacg aggaaagcga
3781 ttcgtcttcc aaatgttctc tgtatgctga ctcagatggc gaaaatgaca gcacatcaga
3841 ctccgagagc agcagctctt ccagctcctc atcctcctcc tcctcctcgt cctcatcctc
3901 ctcgtcctct tcatcctctg agtcctcctc tgaagatgaa gaggaagagg agcggccagc
3961 agcccttccc tcagcctccc cgccccccag agaagtccca gtgcccacgc cagcacctgt
4021 ggaggtgcca gtgccggaaa ggtaagtatc aaggttacaa gacaggttta aggagaccaa
4081 taagctatta ttcgtatagg atactttata cgaagttatt aagttatgga gaccggtgtg
4141 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
4201 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
4261 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
4321 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
4381 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
4441 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
4501 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
4561 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
4621 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
4681 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
4741 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
4801 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
4861 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
4921 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
4981 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
5041 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
5101 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
5161 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
5221 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
5281 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
5341 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
5401 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
5461 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
5521 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
5581 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
5641 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
5701 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
5761 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
5821 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
5881 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
5941 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
6001 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
6061 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
6121 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
6181 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
6241 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
6301 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
6361 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
6421 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
6481 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
6541 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
6601 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
6661 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
6721 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
6781 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
6841 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
6901 t
//
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