| 质粒名称: | pAAVLINK-EF1a-HA-5'-SCN4A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001569 |
| 产品用途: | 5' AAVLINK plasmid for SCN4A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SCN4A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-SCN4A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-SCN4A载体质粒基本信息
pAAVLINK-EF1a-HA-5'-SCN4A质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-SCN4A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-SCN4A质粒载体简介
pAAVLINK-EF1a-HA-5'-SCN4A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-SCN4A 6878 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6878)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6878
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4018
/codon_start=1
/label=SCN4A
/translation="MARPSLCTLVPLGPECLRPFTRESLAAIEQRAVEEEARLQRNKQM
EIEEPERKPRSDLEAGKNLPMIYGDPPPEVIGIPLEDLDPYYSNKKTFIVLNKGKAIFR
FSATPALYLLSPFSVVRRGAIKVLIHALFSMFIMITILTNCVFMTMSDPPPWSKNVEYT
FTGIYTFESLIKILARGFCVDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFR
VLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCV
RWPPPFNDTNTTWYSNDTWYGNDTWYGNEMWYGNDSWYANDTWNSHASWATNDTFDWDA
YISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKTGRNPNYGYTSYDTFSWAFLAL
FRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATL
AEDKEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEADGDPAHGKDCNGSLDTSQGEK
GAPRQSGSGDSGISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWLKFKNIIHLIVM
DPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLTVGNLVFTGIFTAEMVLKLIAMDPY
EYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIG
NSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIALDCNLPRWHMHDFFHSFLI
VFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAA
SDEDGEMNNLQIAIGRIKLGIGFAKAFLLGLLHGKILSPKDIMLSLGEADGAGEAGEAG
ETAPEDEKKEPPEEDLKKDNHILNHMGLADGPPSSLELDHLNFINNPYLTIQVPIASEE
SDLEMPTEEETDTFSEPEDSKKPPQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEGEQ
PEECFTEACVQRWPCLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGAL
AFEDIYIEQRRVIRTILEYADKVFTYIFIMEMLLKWVAYGFK"
misc_feature 849..854
/label=SDS
misc_feature 852..857
/label=SDS
misc_feature 2142..2147
/label=SDS
misc_feature 2184..2189
/label=SDS
misc_feature 3265..3270
/label=SDS
misc_feature 3399..3404
/label=SDS
misc_feature 3561..3566
/label=SDS
misc_feature 3669..3674
/label=SDS
intron 4019..4059
/label=Splicing Donor
misc_feature 4063..4096
/label=lox2272-JT1571
repeat_region 4141..4281
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4141..4270
/label=AAV2 ITR
rep_origin 4356..4811
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5093..5197
/gene="bla"
/label=AmpR promoter
CDS 5198..6058
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6229..6817
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccagacca tctctgtgca ccctggtgcc
781 tctgggccct gagtgcttgc gccccttcac ccgggagtca ctggcagcca tagaacagcg
841 ggcggtggag gaggaggccc ggctgcagcg gaataagcag atggagattg aggagcccga
901 acggaagcca cgaagtgact tggaggctgg caagaaccta cccatgatct acggagaccc
961 cccgccggag gtcatcggca tccccctgga ggacctggat ccctactaca gcaataagaa
1021 gaccttcatc gtactcaaca agggcaaggc catcttccgc ttctccgcca cacctgctct
1081 ctacctgctg agccccttca gcgtagtcag gcgcggggcc atcaaggtgc tcatccatgc
1141 gctgttcagc atgttcatca tgatcaccat cttgaccaac tgcgtattca tgaccatgag
1201 tgacccgcct ccctggtcca agaatgtgga gtacaccttc acagggatct acacctttga
1261 gtccctcatc aagatactgg cccgaggctt ctgtgtcgac gacttcacat tcctccggga
1321 cccctggaac tggctggact tcagtgtcat catgatggcg tacctgacag agtttgtgga
1381 cttgggcaac atctcagccc tgaggacctt ccgggtgctg cgggccctca aaaccatcac
1441 ggtcatccca gggctgaaga cgatcgtggg ggccctgatc cagtcggtga aaaagctgtc
1501 ggatgtgatg atcctcactg tcttctgcct gagcgtcttt gcgctggtag gactgcagct
1561 cttcatggga aacctgaggc agaagtgtgt gcgctggccc ccgccgttca acgacaccaa
1621 caccacgtgg tacagcaatg acacgtggta cggcaatgac acatggtatg gcaatgagat
1681 gtggtacggc aatgactcat ggtatgccaa cgacacgtgg aacagccatg caagctgggc
1741 caccaacgat acctttgatt gggacgccta catcagtgat gaagggaact tctacttcct
1801 ggagggctcc aacgatgccc tgctctgtgg gaacagcagt gatgctgggc actgccctga
1861 gggttatgag tgcatcaaga ccgggcggaa ccccaactat ggctacacca gctatgacac
1921 cttcagctgg gccttcttgg ctctcttccg cctcatgaca caggactatt gggagaacct
1981 cttccagctg acccttcgag cagctggcaa gacctacatg atcttcttcg tggtcatcat
2041 cttcctgggc tctttctacc tcatcaatct gatcctggcc gtggtggcca tggcatatgc
2101 cgagcagaat gaggccaccc tggccgagga taaggagaaa gaggaggagt ttcagcagat
2161 gcttgagaag ttcaaaaagc accaggagga gctggagaag gccaaggccg cccaagctct
2221 ggaaggtggg gaggcagatg gggacccagc ccatggcaaa gactgcaatg gcagcctgga
2281 cacatcgcaa ggggagaagg gagccccgag gcagagcggc agcggagaca gcggcatctc
2341 cgacgccatg gaagaactgg aagaggccca ccaaaagtgc ccaccatggt ggtacaagtg
2401 cgcccacaaa gtgctcatat ggaactgctg cgccccgtgg ctgaagttca agaacatcat
2461 ccacctgatc gtcatggacc cgttcgtgga cctgggcatc accatctgca tcgtgctcaa
2521 caccctcttc atggccatgg aacattaccc catgacggag cactttgaca acgtgctcac
2581 tgtgggcaac ctggtcttca caggcatctt cacagcagag atggttctga agctgattgc
2641 catggacccc tacgagtatt tccagcaggg ttggaatatc ttcgacagca tcatcgtcac
2701 cctcagcctg gtagagctag gcctggccaa cgtacaggga ctgtctgtgc tacgctcctt
2761 ccgtctgctg cgggtcttca agctggccaa gtcgtggcca acgctgaaca tgctcatcaa
2821 gatcattggc aattcagtgg gggcgctggg taacctgacg ctggtgctgg ctatcatcgt
2881 gttcatcttc gccgtggtgg gcatgcagct gtttggcaag agctacaagg agtgcgtgtg
2941 caagattgcc ttggactgca acctgccgcg ctggcacatg catgatttct tccactcctt
3001 cctcatcgtc ttccgcatcc tgtgcgggga gtggatcgag accatgtggg actgcatgga
3061 ggtggccggc caagccatgt gcctcaccgt cttcctcatg gtcatggtca tcggcaatct
3121 tgtggtcctg aacctgttcc tggctctgct gctgagctcc ttcagcgccg acagtctggc
3181 agcctcggat gaggatggcg agatgaacaa cctgcagatt gccatcgggc gcatcaagtt
3241 gggcatcggc tttgccaagg ccttcctcct ggggctgctg catggcaaga tcctgagccc
3301 caaggacatc atgctcagcc tcggggaggc tgacggggcc ggggaggctg gagaggcggg
3361 ggagactgcc cccgaggatg agaagaagga gccgcccgag gaggacctga agaaggacaa
3421 tcacatcctg aaccacatgg gcctggctga cggcccccca tccagcctcg agctggacca
3481 ccttaacttc atcaacaacc cctacctgac catacaggtg cccatcgcct ccgaggagtc
3541 cgacctggag atgcccaccg aggaggaaac cgacactttc tcagagcctg aggatagcaa
3601 gaagccgccg cagcctctct atgatgggaa ctcgtccgtc tgcagcacag ctgactacaa
3661 gccccccgag gaggaccctg aggagcaggc agaggagaac cccgaggggg agcagcctga
3721 ggagtgcttc actgaggcct gcgtgcagcg ctggccctgc ctctacgtgg acatctccca
3781 gggccgtggg aagaagtggt ggactctgcg cagggcctgc ttcaagattg tcgagcacaa
3841 ctggttcgag accttcattg tcttcatgat cctgctcagc agtggggctc tggccttcga
3901 ggacatctac attgagcagc ggcgagtcat tcgcaccatc ctagaatatg ccgacaaggt
3961 cttcacctac atcttcatca tggagatgct gctcaaatgg gtggcctacg gctttaaggt
4021 aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc gtataggata
4081 ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc
4141 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
4201 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
4261 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
4321 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
4381 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
4441 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
4501 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
4561 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
4621 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
4681 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
4741 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
4801 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
4861 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
4921 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
4981 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
5041 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
5101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
5161 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
5221 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
5281 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
5341 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
5401 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
5461 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
5521 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
5581 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
5641 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
5701 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
5761 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
5821 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
5881 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
5941 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
6001 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
6061 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
6121 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
6181 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
6241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
6301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
6361 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
6421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
6481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
6541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
6601 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
6661 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
6721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
6781 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
6841 acggttcctg gccttttgct ggccttttgc tcacatgt
//
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