pMKV057

LOCUS       pMKV057               15986 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2
            and IPT.
ACCESSION   .
VERSION     .
KEYWORDS    pMKV057
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15986)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 15986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15986
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1668..1692
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2993..3622
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4051..5124
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5190..5384
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5620..5642)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5728..5868
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5783..5802
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5883..5900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6054..6642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6134..6153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6729..7523)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7948..7972
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9810..11462
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(9876..9894)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        11879..12058
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        11911..12094
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        14350..14695
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14638..14658
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tttgatcgcc ggcggtaccg agtgtacttc aagtcagtgg gaaatcaata aaatgattat
       61 tttatgaata tatttcattg tgcaagtaga tagaaattac atatgttaca taacacacga
      121 aataaacaaa aaaagacaat ccaaaaacaa acaccccaaa aaaaataatc actttagata
      181 aactcgtatg aggagaggca cgttcagtga ctcgacgatt cccgagcaaa aaaagtctcc
      241 ccgtcacaca tgtagtgggt gacgcaatta tctttaaagt aatccttctg ttgacttgtc
      301 attgataaca tccagtcttc gtcaggattg caaagaatta tagaagggat cccacctttt
      361 attttcttct tttttccata tttagggttg acagtgaaat cagactggca acctattaat
      421 tgcttccaca atgggacgaa cttgaagggg atgtcgtcga tgatattata ggtggcgtgt
      481 tcatcgtagt tggtgaaatc gatggtaccg ttccaatagt tgtgtcgtcc gagacttcta
      541 gcccaggtgg tctttccggt acgagttggt ccgcagatgt agaggctggg gtgtcggatt
      601 ccattccttc cattgtcctt gttaaatcgg ccatccattc aaggtcagat tgagcttgtt
      661 ggtatgagac aggatgtatg taagtataag cgtctatgct tacatggtat agatgggttt
      721 ccctccagga gtgtagatct tcgtggcagc gaagatctga ttctgtgaag ggcgacacat
      781 acggttcagg ttgtggaggg aataatttgt tggctgaata ttccagccat tgaagctttg
      841 ttgcccattc atgagggaat tcttccttga tcatgtcaag atattcctcc ttagacgttg
      901 cagtctggat aatagttctc catcgtgcgt cagatttgcg aggagaaacc ttatgatctc
      961 ggaaatctcc tctggtttta atatctccgt cctttgatat gtaatcaagg acttgtttag
     1021 agtttctagc tggctggata ttagggtgat ttccttcaaa atcgaaaaaa gaaggatccc
     1081 taatacaagg ttttttatca agctggagaa gagcatgata gtgggtagtg ccatcttgat
     1141 gaagctcaga agcaacacca aggaagaaaa taagaaaagg tgtgagtttc tcccagagaa
     1201 actggaataa atcatctctt tgagatgagc acttgggata ggtaaggaaa acatatttag
     1261 attggagtct gaagttctta ctagcagaag gcatgttgtt gtgactccga ggggttgcct
     1321 caaactctat cttataaccg gcgtggaggc atggaggcag gggtattttg gtcattttaa
     1381 tagatagtgg aaaatgacgt ggaatttact taaagacgaa gtctttgcga caaggggggg
     1441 cccacgccga atttaatatt accggcgtgg ccccccctta tcgcgagtgc tttagcacga
     1501 gcggtccaga tttaaagtag aaaatttccc gcccactagg gttaaaggtg ttcacactat
     1561 aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg tatttccgtt
     1621 ggatacgaat tattcgtacg accctcatag tttaaactat cagtgtttga caggatatat
     1681 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     1741 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     1801 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     1861 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     1921 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     1981 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     2041 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     2101 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     2161 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     2221 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     2281 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     2341 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     2401 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     2461 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     2521 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     2581 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     2641 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     2701 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     2761 cggccgggta cgtgttcgag ccgcccgcgc acggctcaac cgtgcggctg catgaaatcc
     2821 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     2881 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     2941 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     3001 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     3061 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     3121 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     3181 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     3241 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     3301 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     3361 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     3421 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     3481 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     3541 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     3601 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     3661 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     3721 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     3781 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     3841 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     3901 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     3961 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg
     4021 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
     4081 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     4141 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     4201 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     4261 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     4321 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     4381 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     4441 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     4501 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     4561 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     4621 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     4681 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     4741 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     4801 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     4861 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     4921 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     4981 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     5041 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     5101 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     5161 ggggaaaaag gtcgaaaagg cctctttcct gtggatagca cgtacattgg gaacccaaag
     5221 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     5281 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     5341 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     5401 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     5461 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     5521 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     5581 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5641 ggaaacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5701 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     5761 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     5821 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     5881 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5941 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6001 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6061 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6121 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6181 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6241 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6301 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6361 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6421 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6481 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6541 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6601 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6661 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
     6721 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
     6781 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
     6841 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
     6901 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
     6961 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
     7021 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
     7081 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
     7141 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
     7201 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
     7261 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
     7321 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
     7381 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
     7441 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
     7501 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
     7561 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     7621 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     7681 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     7741 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     7801 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     7861 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     7921 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     7981 gcttagacaa cttaataaca cattgcggac gtttttaatg tagagctcaa agtttaacgc
     8041 gttagcagaa ggcatgttgt tgtgactccg aggggttgcc tcaaactcta tcttataacc
     8101 ggcgtggagg catggaggca ggggtatttt ggtcatttta atagatagtg gaaaatgacg
     8161 tggaatttac ttaaagacga agtctttgcg acaagggggg gcccacgccg aatttaatat
     8221 taccggcgtg gccccccctt atcgcgagtg ctttagcacg agcggtccag atttaaagta
     8281 gaaaatttcc cgcccactag ggttaaaggt gttcacacta taaaagcata tacgatgtga
     8341 tggtatttga tggagcgtat attgtatcag gtatttccgt tggatacgaa ttattcgtac
     8401 gaccctcggt accgatcggc gcgcgcggcc gcacagagat ttaaatagct ccggtgacgg
     8461 acggcgcgcc ctatgtcgag ctgcaggtca acggatcagg atattcttgt ttaagatgtt
     8521 gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc ccttcttcat
     8581 agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta ttaatgaaaa
     8641 aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc ccaaataaga
     8701 tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca cttgcctttt
     8761 ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa atcaataatc
     8821 atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc acaatatgtt
     8881 atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc acttgcatta
     8941 gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat tctagaaaat
     9001 acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt ttcagctcca
     9061 ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa cgatcgttaa
     9121 atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac gagtcagtaa
     9181 taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca actcaaagca
     9241 caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt tgcgtgtaaa
     9301 caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct tagctttctc
     9361 gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac ccaaagagct
     9421 cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc tctcaattct
     9481 ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct tcgattttgt
     9541 tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa tcttagatcg
     9601 aacacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta gggtttcata
     9661 gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc ttcgatttgt
     9721 gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg tcgattaatc
     9781 tgagtttttc tgattaacag gcctgcagga tggaagacgc caaaaacata aagaaaggcc
     9841 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
     9901 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
     9961 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
    10021 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
    10081 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
    10141 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
    10201 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
    10261 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
    10321 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
    10381 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
    10441 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
    10501 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
    10561 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
    10621 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
    10681 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
    10741 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
    10801 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
    10861 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
    10921 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
    10981 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
    11041 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
    11101 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
    11161 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgct ggtgtcgcag
    11221 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
    11281 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
    11341 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
    11401 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt
    11461 gactcgaggt tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg
    11521 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga
    11581 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt
    11641 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt
    11701 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa
    11761 acacttgtag ttgtgttaga gctcccgggc gcgccgatca tgagcggaga attaagggag
    11821 tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga
    11881 accgcaacgt tgaaggagcc actcagccgc gggtttctgg agtttaatga gctaagcaca
    11941 tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa
    12001 tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga
    12061 gtctcatatt cactctcaat ccaaataatc tgcaccgtac ctgcagggtc cgagctaggt
    12121 cacagaagcg ctcaggaagg ccgctgagat agaggcatgg cggccaatgc gggcggcggt
    12181 ggagcgggag gaggcagcgg cagcggcagc gtggctgcgc cggcggtgtg ccgccccagc
    12241 ggctcgcggt ggacgccgac gccggagcag atcaggatgc tgaaggagct gtactacggc
    12301 tgcggcatcc ggtcgcccag ctcggagcag atccagcgca tcaccgccat gctgcggcag
    12361 cacggcaaga tcgagggcaa gaacgtcttc tactggttcc agaaccacaa ggcccgcgag
    12421 cgccagaagc gccgcctcac cagcctcgac gtgaacgtgc ccgccgccgg cgcggccgac
    12481 gccaccacca gccaactcgg cgtcctctcg ctgtcgtcgc cgccgccttc aggcgcggcg
    12541 cctccctcgc ccaccctcgg cttctacgcc gccggcaatg gcggcggatc ggctgtgctg
    12601 ctggacacga gttccgactg gggcagcagc ggcgctgcga tggccaccga gacatgcttc
    12661 ctccaggact acatgggcgt gacggacacg ggcagctcgt cgcagtggcc acgcttctcg
    12721 tcgtcggaca cgataatggc ggcggccgcg gcgcgggcgg cgacgacgcg ggcgcccgag
    12781 actctccctc tcttcccgac ctgcggcgac gacggcggca gcggtagcag cagctacttg
    12841 ccgttctggg gtgccgcgtc cacaactgcc ggcgccactt cttccgttgc gatccagcag
    12901 caacaccagc tgcaggagca gtacagcttt tacagcaaca gcaacagcac ccagctggcc
    12961 ggcaccggca accaagacgt atcggcaaca gcagcagcag ccgccgccct ggagctgagc
    13021 ctcagctcat ggtgctcccc ttaccctgct gcagggagta tgtgagagca acgcgagctg
    13081 ccactgctct tcacttatgt ctctggaatg gaaggaggag gaagtgagca tagcgttggt
    13141 gcgttgctgt cattgtccta ggttagtagc tagtgccagt tactagtaag catcaggcat
    13201 aggagtatgt agtagaagca tgcacgttgc cggccagcca ggctttagac gggaaaagaa
    13261 tttggtgcag ccggctgcaa aacaggatgt ttacagcccc cccctcgagc cctagacttg
    13321 tccatcttct ggattggcca agttaattaa tgtatgaaat aaaaggatgc acacatagtg
    13381 acatgctaat cactataatg tgggcatcaa agttgtgtgt tatgtgtaat tactaattat
    13441 ctgaataaga gaaagagatc atccatattt cttatcctaa atgaatgtca cgtgtcttta
    13501 taattctttg atgaaccaga tgcattttat taaccaattc catatacata taaatattaa
    13561 tcatatataa ttaatatcaa ttgggttagc aaaacaaatc tagtctaggt gtgttttgct
    13621 aattattggg ggatagtgca aaaagaaatc tacgttctca ataattcaga tagaaaactt
    13681 aataaagtga gataatttac atagattgct tttatccttt gatatatgtg aaaccatgca
    13741 tgatataagg aaaatagata gagaaataat tttttacatc gttgaatatg taaacaattt
    13801 aattcaagaa gctaggaata taaatattga ggagtttatg attagagctc tcccggcgcg
    13861 ccagatttgc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
    13921 cgcagcaggt atcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
    13981 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
    14041 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
    14101 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
    14161 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
    14221 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
    14281 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
    14341 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
    14401 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
    14461 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
    14521 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
    14581 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
    14641 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
    14701 gactcctgca ggatggatct gcgtctaatt ttcggtccaa cttgcacagg aaagacgtcg
    14761 accgcgatac gtcttgccca gcagactggc cttccagtcc tttcgctcga tcgggtccaa
    14821 tgctgtcctc aactgtcaac cggaagcgga cgaccaacag tggaagaact gaaaggaacg
    14881 acccgtctat accttgaaga tcggcctctg gtgaagggta tcatcgcagc caagcaagct
    14941 cacgaaaggc tgatcgggga agtgtacaat tatgaggccc acggcgggct tattcttgag
    15001 ggaggatcta tctcgttgct caggtgcatg gcgcaaagca gttattggag taccgatttt
    15061 cgttggcata ttattcgcca caagttagca gacgaggaga cattcatgaa cgcggccaag
    15121 gccagagtta ggcagatgtt gcgccctgct gtaggcccat ctattattca agagttggtt
    15181 catctttgga atgagcctcg gctgaggccc atactgaaag agatcgacgg atatcgatat
    15241 gccatgttat ttgctagcca gaaccagatc acacccgata tgctattgca gcttgaccca
    15301 gatatggagg gtgagttgat tcatggaatc gctcaggagt atctcatcca tgcgcgccgg
    15361 caggagcagg aattccctcc agtgagcgtg gtcgctttcg aaggattcga aggtccaccg
    15421 ttcggaatgt gctagctcga gccctagact tgtccatctt ctggattggc caagttaatt
    15481 aatgtatgaa ataaaaggat gcacacatag tgacatgcta atcactataa tgtgggcatc
    15541 aaagttgtgt gttatgtgta attactaatt atctgaataa gagaaagaga tcatccatat
    15601 ttcttatcct aaatgaatgt cacgtgtctt tataattctt tgatgaacca gatgcatttt
    15661 attaaccaat tccatataca tataaatatt aatcatatat aattaatatc aattgggtta
    15721 gcaaaacaaa tctagtctag gtgtgttttg ctaattattg ggggatagtg caaaaagaaa
    15781 tctacgttct caataattca gatagaaaac ttaataaagt gagataattt acatagattg
    15841 cttttatcct ttgatatatg tgaaaccatg catgatataa ggaaaataga tagagaaata
    15901 attttttaca tcgttgaata tgtaaacaat ttaattcaag aagctaggaa tataaatatt
    15961 gaggagttta tgattagagc tcagtg
//