pC0046-EF1a-PspCas13b-NES-HIV

LOCUS       pC0046-EF1a-PspCas13b-NES-HIV               12607 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Expresses PspCas13b in mammalian cells for knockdown of target RNAs 
            in combination with compatible guide RNAs. Localized to the 
            cytoplasm.
ACCESSION   .
VERSION     .
KEYWORDS    pC0046-EF1a-PspCas13b-NES-HIV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung 
            J, Zhang F
  TITLE     RNA editing with CRISPR-Cas13.
  JOURNAL   Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
  PUBMED    29070703
REFERENCE   2  (bases 1 to 12607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12607
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2445..3623
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2676..3614
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3571..3591
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6918..6944
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             6954..7034
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    7062..7650
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7115..7135)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7533..7544)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7653..7669)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7654..7670)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8175..8355
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8381..8398)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8387..8611
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8657..9085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8744..8763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8954..8975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9094..9114)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9099..9428
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9279..9414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9341..9360
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9476..9523
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9542..9916
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10046..10167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10083..10102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10137..10156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10216..10232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10216..10232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10229..10251)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10240..10256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10264..10294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10309..10330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10447..10464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10618..11206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10698..10717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11377..12237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12000..12019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12238..12342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12417..12436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaatgcaaa gatggataaa gttttaaaca gagaggaatc
     2401 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2461 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2521 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2581 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2641 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2701 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2761 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2821 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2881 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2941 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     3001 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     3061 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     3121 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     3181 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     3241 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3301 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3361 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3421 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3481 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3541 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3601 ttcttccatt tcaggtgtcg tgacgtacgg ccaccatgaa catccccgct ctggtggaaa
     3661 accagaagaa gtactttggc acctacagcg tgatggccat gctgaacgct cagaccgtgc
     3721 tggaccacat ccagaaggtg gccgatattg agggcgagca gaacgagaac aacgagaatc
     3781 tgtggtttca ccccgtgatg agccacctgt acaacgccaa gaacggctac gacaagcagc
     3841 ccgagaaaac catgttcatc atcgagcggc tgcagagcta cttcccattc ctgaagatca
     3901 tggccgagaa ccagagagag tacagcaacg gcaagtacaa gcagaaccgc gtggaagtga
     3961 acagcaacga catcttcgag gtgctgaagc gcgccttcgg cgtgctgaag atgtacaggg
     4021 acctgaccaa ccactacaag acctacgagg aaaagctgaa cgacggctgc gagttcctga
     4081 ccagcacaga gcaacctctg agcggcatga tcaacaacta ctacacagtg gccctgcgga
     4141 acatgaacga gagatacggc tacaagacag aggacctggc cttcatccag gacaagcggt
     4201 tcaagttcgt gaaggacgcc tacggcaaga aaaagtccca agtgaatacc ggattcttcc
     4261 tgagcctgca ggactacaac ggcgacacac agaagaagct gcacctgagc ggagtgggaa
     4321 tcgccctgct gatctgcctg ttcctggaca agcagtacat caacatcttt ctgagcaggc
     4381 tgcccatctt ctccagctac aatgcccaga gcgaggaacg gcggatcatc atcagatcct
     4441 tcggcatcaa cagcatcaag ctgcccaagg accggatcca cagcgagaag tccaacaaga
     4501 gcgtggccat ggatatgctc aacgaagtga agcggtgccc cgacgagctg ttcacaacac
     4561 tgtctgccga gaagcagtcc cggttcagaa tcatcagcga cgaccacaat gaagtgctga
     4621 tgaagcggag cagcgacaga ttcgtgcctc tgctgctgca gtatatcgat tacggcaagc
     4681 tgttcgacca catcaggttc cacgtgaaca tgggcaagct gagatacctg ctgaaggccg
     4741 acaagacctg catcgacggc cagaccagag tcagagtgat cgagcagccc ctgaacggct
     4801 tcggcagact ggaagaggcc gagacaatgc ggaagcaaga gaacggcacc ttcggcaaca
     4861 gcggcatccg gatcagagac ttcgagaaca tgaagcggga cgacgccaat cctgccaact
     4921 atccctacat cgtggacacc tacacacact acatcctgga aaacaacaag gtcgagatgt
     4981 ttatcaacga caaagaggac agcgccccac tgctgcccgt gatcgaggat gatagatacg
     5041 tggtcaagac aatccccagc tgccggatga gcaccctgga aattccagcc atggccttcc
     5101 acatgtttct gttcggcagc aagaaaaccg agaagctgat cgtggacgtg cacaaccggt
     5161 acaagagact gttccaggcc atgcagaaag aagaagtgac cgccgagaat atcgccagct
     5221 tcggaatcgc cgagagcgac ctgcctcaga agatcctgga tctgatcagc ggcaatgccc
     5281 acggcaagga tgtggacgcc ttcatcagac tgaccgtgga cgacatgctg accgacaccg
     5341 agcggagaat caagagattc aaggacgacc ggaagtccat tcggagcgcc gacaacaaga
     5401 tgggaaagag aggcttcaag cagatctcca caggcaagct ggccgacttc ctggccaagg
     5461 acatcgtgct gtttcagccc agcgtgaacg atggcgagaa caagatcacc ggcctgaact
     5521 accggatcat gcagagcgcc attgccgtgt acgatagcgg cgacgattac gaggccaagc
     5581 agcagttcaa gctgatgttc gagaaggccc ggctgatcgg caagggcaca acagagcctc
     5641 atccatttct gtacaaggtg ttcgcccgca gcatccccgc caatgccgtc gagttctacg
     5701 agcgctacct gatcgagcgg aagttctacc tgaccggcct gtccaacgag atcaagaaag
     5761 gcaacagagt ggatgtgccc ttcatccggc gggaccagaa caagtggaaa acacccgcca
     5821 tgaagaccct gggcagaatc tacagcgagg atctgcccgt ggaactgccc agacagatgt
     5881 tcgacaatga gatcaagtcc cacctgaagt ccctgccaca gatggaaggc atcgacttca
     5941 acaatgccaa cgtgacctat ctgatcgccg agtacatgaa gagagtgctg gacgacgact
     6001 tccagacctt ctaccagtgg aaccgcaact accggtacat ggacatgctt aagggcgagt
     6061 acgacagaaa gggctccctg cagcactgct tcaccagcgt ggaagagaga gaaggcctct
     6121 ggaaagagcg ggcctccaga acagagcggt acagaaagca ggccagcaac aagatccgca
     6181 gcaaccggca gatgagaaac gccagcagcg aagagatcga gacaatcctg gataagcggc
     6241 tgagcaacag ccggaacgag taccagaaaa gcgagaaagt gatccggcgc tacagagtgc
     6301 aggatgccct gctgtttctg ctggccaaaa agaccctgac cgaactggcc gatttcgacg
     6361 gcgagaggtt caaactgaaa gaaatcatgc ccgacgccga gaagggaatc ctgagcgaga
     6421 tcatgcccat gagcttcacc ttcgagaaag gcggcaagaa gtacaccatc accagcgagg
     6481 gcatgaagct gaagaactac ggcgacttct ttgtgctggc tagcgacaag aggatcggca
     6541 acctgctgga actcgtgggc agcgacatcg tgtccaaaga ggatatcatg gaagagttca
     6601 acaaatacga ccagtgcagg cccgagatca gctccatcgt gttcaacctg gaaaagtggg
     6661 ccttcgacac ataccccgag ctgtctgcca gagtggaccg ggaagagaag gtggacttca
     6721 agagcatcct gaaaatcctg ctgaacaaca agaacatcaa caaagagcag agcgacatcc
     6781 tgcggaagat ccggaacgcc ttcgatcaca acaattaccc cgacaaaggc gtggtggaaa
     6841 tcaaggccct gcctgagatc gccatgagca tcaagaaggc ctttggggag tacgccatca
     6901 tgaagggatc ccttcaactg cctccacttg aaagactgac actgggtagt tcctacccat
     6961 acgatgttcc agattacgct tatccctacg acgtgcctga ttatgcatac ccatatgatg
     7021 tccccgacta tgcctaagaa ttcgatatca agcttatcga taatcaacct ctggattaca
     7081 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7141 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7201 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7261 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7321 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7381 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7441 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7501 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7561 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7621 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     7681 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7741 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     7801 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7861 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     7921 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     7981 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     8041 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     8101 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     8161 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8221 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8281 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8341 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     8401 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     8461 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     8521 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     8581 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     8641 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8701 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8761 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8821 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8881 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8941 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9001 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9061 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     9121 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9181 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9241 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9361 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9421 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     9481 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     9541 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     9601 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     9661 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     9721 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     9781 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     9841 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     9901 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     9961 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
    10021 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
    10081 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10141 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
    10201 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10261 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10321 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10381 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10441 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10501 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10561 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10621 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    10801 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    10861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    10921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11041 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11101 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11161 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11401 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    11761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    11821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    11881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    11941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12361 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    12421 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    12481 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
    12541 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
    12601 acgcgtt
//