| 质粒名称: | proE-cad178-Luc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 42081 |
| 存储实验室: | Kumiko Ui-Tei |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3-Basic |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4807 bp |
| 完整质粒大小: | 5077 bp |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | E-cadherin,CDH1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 270 bp |
| 基因突变: | contains E-cadherin promoter region from -178 to +92 |
| GenBank编号 | NM_004360 |
| 启动子 | E-cadherin |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII(未破坏) |
| 3' 克隆位点: | HindIII(未破坏) |
| 5' 测序引物 | CTAGCAAAATAGGCTGTCCC |
|
文献引用方法 |
|
| 材料和方法部分: | proE-cad178-Luc was a gift from Kumiko Ui-Tei (Addgene plasmid # 42081 ; http://n2t.net/addgene:42081 ; RRID:Addgene_42081) |
| 参考文献部分: | E-cadherin is transcriptionally activated via suppression of ZEB1 transcriptional repressor by small RNA-mediated gene silencing. Mazda M, Nishi K, Naito Y, Ui-Tei K. PLoS One. 2011;6(12):e28688. doi: 10.1371/journal.pone.0028688. Epub 2011 Dec 21. 10.1371/journal.pone.0028688 PubMed 22205962 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS proE-cad178-Luc 5077 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS proE-cad178-Luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5077)
AUTHORS Mazda M, Nishi K, Naito Y, Ui-Tei K
TITLE E-cadherin is transcriptionally activated via suppression of ZEB1
transcriptional repressor by small RNA-mediated gene silencing.
JOURNAL PLoS One. 2011;6(12):e28688. doi: 10.1371/journal.pone.0028688. Epub
2011 Dec 21.
PUBMED 22205962
REFERENCE 2 (bases 1 to 5077)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5077
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 341..350
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 347..1999
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(413..431)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 2040..2161
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2051..2070)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2105..2124
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2409..2426)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2580..3168)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2660..2679)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3339..4199)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3962..3981
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4200..4304)
/gene="bla"
/label=AmpR promoter
rep_origin 4331..4786
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4418..4437)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4628..4649
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4917..4965
/note="synthetic polyadenylation signal"
misc_feature 4979..5070
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5019..5038
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc ttccaggcta gagggtcacc
61 gcgtctatgc gaggccgggt gggcgggccg tcagctccgc cctggggagg ggtccgcgct
121 gctgattggc tgtggccggc aggtgaaccc tcagccaatc agcggtacgg ggggcggtgc
181 ctccggggct cacctggctg cagccacgca ccccctctca gtggcgtcgg aactgcaaag
241 cacctgtgag cttgcggaag tcagttcaga ctccagcccg ctccagcccg gcccgacccg
301 accgcacccg gaagcttggc attccggtac tgttggtaaa gccaccatgg aagacgccaa
361 aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca
421 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc
481 acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga
541 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc
601 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc
661 gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt
721 ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat
781 catccaaaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta
841 cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc
901 cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc
961 taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc
1021 tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca
1081 cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat
1141 gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc
1201 gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga
1261 tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga
1321 agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac
1381 tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt
1441 tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa
1501 tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc
1561 ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta
1621 ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa
1681 aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt
1741 cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt
1801 tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca
1861 agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg
1921 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg
1981 cggaaagatc gccgtgtaat tctagagtcg gggcggccgg ccgcttcgag cagacatgat
2041 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
2101 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
2161 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
2221 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc
2281 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
2341 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
2401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
2461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
2521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
2581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
2641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
2701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
2761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
2821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
2881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
2941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
3001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
3061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
3121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
3181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
3241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
3301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
3361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
3421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
3481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
3541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
3601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
3661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
3721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
3781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
3841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
3901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
3961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
4021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
4081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
4141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
4201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
4261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
4321 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
4381 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
4441 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
4501 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
4561 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
4621 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
4681 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
4741 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc
4801 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc
4861 aagctaccat gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata
4921 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
4981 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
5041 gtgcaagtgc aggtgccaga acatttctct atcgata
//
