| 出品公司: | Thermofisher |
|---|---|
| 载体名称: | pMCS-Gaussia-Luc |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 4.8 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 产品目录号: | #16146 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 载体序列: | pMCS-Gaussia Luc |
| 备注: |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pMCS-Gaussia Luc 载体是一种设计用于接受启动子序列的多克隆位点质粒，用于利用自然分泌 Gaussia 荧光素酶报告基因进行的基因调控研究。
载体序列
LOCUS pMCS-Gaussia Luc 4886 bp ds-DNA circular SYN 29-FEB-2024
DEFINITION Secreted Gaussia luciferase vector for cloning a transcriptional
regulatory element.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4886)
AUTHORS Thermo Fisher (Pierce)
TITLE Direct Submission
JOURNAL Exported Thursday, Feb 29, 2024 from SnapGene 4.3.6 to Vector NTI(R)
format
https://www.biofeng.com
COMMENT VNTDATE|666144000|
COMMENT VNTDBDATE|1050364800|
COMMENT VNTNAME|pMCS-Gaussia Luc|
COMMENT VNTAUTHORNAME|Thermo Fisher (Pierce)|
FEATURES Location/Qualifiers
misc_feature 1..42
/label=MCS
/note="multiple cloning site"
CDS 50..607
/codon_start=1
/product="secreted Gaussia luciferase"
/label=hGLuc
/note="human codon-optimized"
polyA_signal 630..741
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 864..1193
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1044..1179
/label=SV40 ori
/note="SV40 origin of replication"
promoter 1241..1288
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 1307..1906
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
polyA_signal 2036..2157
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(2210..3070)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 3334..3922
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4664..4680
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 4795..4886
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 actagtgaat tcggtaccga gctcctcgag aagcttggat ccagccacca tgggagtcaa
61 agttctgttt gccctgatct gcatcgctgt ggccgaggcc aagcccaccg agaacaacga
121 agacttcaac atcgtggccg tggccagcaa cttcgcgacc acggatctcg atgctgaccg
181 cgggaagttg cccggcaaga agctgccgct ggaggtgctc aaagagatgg aagccaatgc
241 ccggaaagct ggctgcacca ggggctgtct gatctgcctg tcccacatca agtgcacgcc
301 caagatgaag aagttcatcc caggacgctg ccacacctac gaaggcgaca aagagtccgc
361 acagggcggc ataggcgagg cgatcgtcga cattcctgag attcctgggt tcaaggactt
421 ggagcccatg gagcagttca tcgcacaggt cgatctgtgt gtggactgca caactggctg
481 cctcaaaggg cttgccaacg tgcagtgttc tgacctgctc aagaagtggc tgccgcaacg
541 ctgtgcgacc tttgccagca agatccaggg ccaggtggac aagatcaagg gggccggtgg
601 tgactaagcg gccgcaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt
661 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
721 ataaaatgag gaaattgcat cacaacactc aaccctatct cggtctattc ttttgattta
781 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
841 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
901 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
961 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
1021 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
1081 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
1141 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
1201 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
1261 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatga ccgagtacaa
1321 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
1381 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
1441 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
1501 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
1561 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
1621 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
1681 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
1741 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga catccgcgcc
1801 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
1861 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacacg tgctacgaga
1921 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
1981 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
2041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
2101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
2161 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat taccaatgct
2221 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
2281 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agcgctgcga
2341 tgataccgcg agaaccacgc tcaccggctc cggatttatc agcaataaac cagccagccg
2401 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
2461 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
2521 tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
2581 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
2641 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
2701 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
2761 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
2821 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
2881 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
2941 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
3001 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
3061 gaatactcat attcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
3121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtc agtgttacaa
3181 ccaattaacc aattctgaac attatcgcga gcccatttat acctgaatat ggctcataac
3241 accccttgct catgaccaaa atcccttaac gtgagttacg cgcgcgtcgt tccactgagc
3301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
3361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
3421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
3481 tcttctagtg tagccgtagt tagcccacca cttcaagaac tctgtagcac cgcctacata
3541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
3601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
3661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
3721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
3781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
3841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
3901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
3961 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
4021 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
4081 gtcagtgagc gaggaagcgg aaggcgagag tagggaactg ccaggcatca aactaagcag
4141 aaggcccctg acggatggcc tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga
4201 cggccagtct taagctcggg ccccctgggc ggttctgata acgagtaatc gttaatccgc
4261 aaataacgta aaaacccgct tcggcgggtt tttttatggg gggagtttag ggaaagagca
4321 tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat atatgagaat tatttaacct
4381 tataaatgag aaaaaagcaa cgcactttaa ataagatacg ttgctttttc gattgatgaa
4441 cacctataat taaactattc atctattatt tatgattttt tgtatataca atatttctag
4501 tttgttaaag agaattaaga aaataaatct cgaaaataat aaagggaaaa tcagtttttg
4561 atatcaaaat tatacatgtc aacgataata caaaatataa tacaaactat aagatgttat
4621 cagtatttat tatcatttag aataaatttt gtgtcgccct taattgtgag cggataacaa
4681 ttacgagctt catgcacagt ggcgttgaca ttgattattg actagcatgt tctttcctgc
4741 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taataacata
4801 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
4861 aagtgcaggt gccagaacat ttctct
//
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