| 质粒名称: | pAAVLINK-EF1a-HA-5'-RAI1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001413 |
| 产品用途: | 5' AAVLINK plasmid for RAI1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RAI1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-RAI1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-RAI1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-RAI1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-RAI1质粒载体简介
pAAVLINK-EF1a-HA-5'-RAI1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-RAI1 7135 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7135)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7135
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4297
/codon_start=1
/label=RAI1
/translation="QSFRERCGFHGKQQNYQQTSQETSRLENYRQPSQAGLSCDRQRLL
AKDYYNPQPYPSYEGGAGTPSGTAAAVAADKYHRGSKALPTQQGLQGRPAFPGYGVQDS
SPYPGRYAGEESLQAWGAPQPPPPQPQPLPAGVAKYDENLMKKTAVPPSRQYAEQGAQV
PFRTHSLHVQQPPPPQQPLAYPKLQRQKLQNDIASPLPFPQGTHFPQHSQSFPTSSTYS
SSVQGGGQGAHSYKSCTAPTAQPHDRPLTASSSLAPGQRVQNLHAYQSGRLSYDQQQQQ
QQQQQQQQQALQSRHHAQETLHYQNLAKYQHYGQQGQGYCQPDAAVRTPEQYYQTFSPS
SSHSPARSVGRSPSYSSTPSPLMPNLENFPYSQQPLSTGAFPAGITDHSHFMPLLNPSP
TDATSSVDTQAGNCKPLQKDKLPENLLSDLSLQSLTALTSQVENISNTVQQLLLSKAAV
PQKKGVKNLVSRTPEQHKSQHCSPEGSGYSAEPAGTPLSEPPSSTPQSTHAEPQEADYL
SGSEDPLERSFLYCNQARGSPARVNSNSKAKPESVSTCSVTSPDDMSTKSDDSFQSLHG
SLPLDSFSKFVAGERDCPRLLLSALAQEDLASEILGLQEAIGEKADKAWAEAPSLVKDS
SKPPFSLENHSACLDSVAKSAWPRPGEPEALPDSLQLDKGGNAKDFSPGLFEDPSVAFA
TPDPKKTTGPLSFGTKPTLGVPAPDPTTAAFDCFPDTTAASSADSANPFAWPEENLGDA
CPRWGLHPGELTKGLEQGGKASDGISKGDTHEASACLGFQEEDPPGEKVASLPGDFKQE
EVGGVKEEAGGLLQCPEVAKADRWLEDSRHCCSTADFGDLPLLPPTSRKEDLEAEEEYS
SLCELLGSPEQRPGMQDPLSPKAPLICTKEEVEEVLDSKAGWGSPCHLSGESVILLGPT
VGTESKVQSWFESSLSHMKPGEEGPDGERAPGDSTTSDASLAQKPNKPAVPEAPIAKKE
PVPRGKSLRSRRVHRGLPEAEDSPCRAPVLPKDLLLPESCTGPPQGQMEGAGAPGRGAS
EGLPRMCTRSLTALSEPRTPGPPGLTTTPAPPDKLGGKQRAAFKSGKRVGKPSPKAASS
PSNPAALPVASDSSPMGSKTKETDSPSTPGKDQRSMILRSRTKTQEIFHSKRRRPSEGR
LPNCRATKKLLDNSHLPATFK"
misc_feature 1386..1391
/label=SDS
misc_feature 1401..1406
/label=SDS
misc_feature 2271..2276
/label=SDS
misc_feature 2544..2549
/label=SDS
misc_feature 3114..3119
/label=SDS
misc_feature 3117..3122
/label=SDS
misc_feature 3168..3173
/label=SDS
misc_feature 3171..3176
/label=SDS
misc_feature 3189..3194
/label=SDS
misc_feature 3192..3197
/label=SDS
misc_feature 3318..3323
/label=SDS
misc_feature 3336..3341
/label=SDS
misc_feature 3435..3440
/label=SDS
misc_feature 3438..3443
/label=SDS
misc_feature 3447..3452
/label=SDS
intron 4298..4338
/label=Splicing Donor
misc_feature 4342..4375
/label=lox2272-JT1571
repeat_region 4420..4538
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4420..4527
/label=AAV2 ITR
rep_origin 4613..5068
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5350..5454
/gene="bla"
/label=AmpR promoter
CDS 5455..6315
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6486..7074
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctcagt cttttcgaga aaggtgtggt ttccatggca aacaacagaa
781 ctaccagcag acctcgcagg aaacatcacg cctagagaat tacaggcagc cgagtcaggc
841 cgggctaagc tgcgaccggc agcggctgct cgccaaggac tattataacc cgcagcctta
901 cccgagctat gagggtggcg ctggcacgcc ctctggcact gcagccgcgg tggccgccga
961 caagtaccac cgaggcagca aggccctgcc cacacagcaa ggcctgcagg ggaggccggc
1021 tttccctggc tacggcgtcc aggacagcag cccctaccca ggccgctatg ctggtgagga
1081 gagccttcag gcttgggggg ccccacagcc accaccccca cagccgcagc cactacctgc
1141 aggggtggcc aagtatgatg agaacttgat gaaaaagaca gcagtgcccc ccagcaggca
1201 gtatgcagag cagggcgccc aggtgccctt tcggactcac tccctgcacg tccagcagcc
1261 accgccgccc cagcagcccc tggcataccc caagctccaa aggcagaagc tgcagaacga
1321 cattgcctcc cctctgccct tcccccaggg tacccacttt cctcagcatt cccagtcctt
1381 ccccacctcc tccacctact cctcctctgt ccagggtggt gggcaggggg cccactccta
1441 taagagttgc acagcaccga ctgcccagcc ccatgacagg ccgctgactg ccagctccag
1501 cctggccccg gggcagcggg tccagaatct tcatgcctac cagtcgggcc gcctcagcta
1561 tgaccagcag cagcagcagc agcagcagca gcagcagcag cagcaagccc ttcagagccg
1621 gcaccatgcc caggaaaccc tccattacca aaacctcgcc aagtatcagc actacgggca
1681 gcaaggccag ggctactgcc agccggacgc agccgtccgg accccagagc agtactacca
1741 gaccttcagc cccagctcca gccactcacc cgcccgctcc gtgggccgct caccttccta
1801 cagttccaca ccgtcgccgc tgatgccaaa cctggagaac tttccctaca gccagcagcc
1861 gctcagcacc ggggccttcc ccgcagggat cactgaccac agccacttca tgcccctgct
1921 caatccctcc ccaacggatg ccaccagctc tgtggacacc caggctggca actgcaagcc
1981 ccttcagaag gacaagctcc ctgagaacct gctgtcggat ctcagcctgc agagcctcac
2041 ggcgctgacc tcacaggtgg agaacatctc caacaccgtc cagcagctgc tgctctccaa
2101 ggctgctgtg ccgcagaaga aaggtgtcaa gaacctcgtg tccaggaccc cagagcagca
2161 taaaagccag cactgcagcc ccgaagggag cggctactca gccgagcccg caggcacacc
2221 gctgtcagag ccgccgagca gcacgccaca gtccacgcat gcggagccgc aggaggccga
2281 ctacctgagc ggctccgagg acccactgga gcgcagcttc ctctactgca accaggcccg
2341 tggcagccct gccagggtca acagcaactc gaaggccaag cccgagtccg tgtccacctg
2401 ttctgtgacc tctcctgacg acatgtccac caaatctgac gactccttcc agagcctaca
2461 cggcagtctg ccgctcgaca gcttctccaa gttcgtggcg ggtgagcggg actgtccgcg
2521 gctgctgctc agcgccctgg cacaggagga cctggcctcc gagatcctgg ggctgcagga
2581 agccatcggt gagaaggccg acaaagcttg ggctgaagca cccagcctgg tcaaggacag
2641 cagcaagcca cccttctcgc tggagaacca cagcgcctgc ctggactctg tggccaagag
2701 tgcgtggccc cggcctgggg agccagaggc cctgcccgac tccttgcagc tggacaaggg
2761 cggcaatgcc aaggacttca gcccagggct gtttgaagac ccttccgtgg ccttcgctac
2821 gcctgacccc aaaaagacaa ctggtcctct ctcctttggt accaagccca cccttggggt
2881 tcctgctcca gaccccacta cagcagcttt tgactgtttc ccggacacaa ccgctgccag
2941 ctcagcggac agcgccaacc cctttgcctg gccagaggaa aacctggggg atgcttgtcc
3001 caggtgggga ttgcaccctg gcgagcttac caagggcctg gagcagggtg ggaaggcctc
3061 agatggcatc agcaaagggg acacccatga ggcttcggcc tgcctgggct tccaggagga
3121 ggacccccct ggggagaagg tggcctcgtt gcccggggac ttcaagcagg aggaggtggg
3181 tggggtgaag gaggaggcag gtgggctgct gcagtgcccc gaggtggcca aggctgaccg
3241 gtggctggag gacagccggc actgctgttc caccgccgac ttcggggacc tcccactgct
3301 gccacccacc agcaggaagg aggacctgga agctgaggag gagtactcct ccctatgtga
3361 gctcctgggc agccccgagc agaggcctgg catgcaggac ccgctgtcac ccaaggcccc
3421 actcatctgc accaaggagg aggtggagga ggtgctggac tccaaggccg gctggggctc
3481 tccgtgccac ctctcagggg agtccgtcat cctgctgggc cctacagtgg gcaccgagtc
3541 aaaggtccag agctggtttg agtcctctct gtcacacatg aagccaggtg aagaggggcc
3601 tgatggggag cgagctccag gggattccac cacctcggac gcctctctgg cccagaagcc
3661 caacaagcct gctgtgcccg aggcgcccat cgcaaagaaa gagcctgtgc cacggggcaa
3721 aagcttacgg agccgtcggg tgcaccgggg gctgcccgag gccgaggact ccccatgcag
3781 ggcaccagtg ctgcccaaag acctcttgct ccctgaatcc tgcacagggc ccccccaggg
3841 acagatggaa ggggctggag ccccaggccg gggggcctcg gaagggctcc ccaggatgtg
3901 tactcgttct ctcacggccc tgagtgagcc ccgcacgccc ggacccccag gcctgaccac
3961 cacccctgca cccccagaca aactgggggg caagcagcga gccgccttca agtcgggcaa
4021 gcgggtgggg aagccctcac ccaaggctgc ctccagcccc agcaacccgg ccgccctgcc
4081 tgtggcctcc gacagcagcc cgatgggctc caagaccaag gagacagact cacccagcac
4141 gcctggcaag gaccagcgct ccatgatcct tcggtcacgc accaaaaccc aggagatctt
4201 ccactccaag cggcggaggc cctctgaggg ccggctcccc aactgccgtg ccaccaagaa
4261 gctcctcgac aacagccact tgcccgccac attcaaggta agtatcaagg ttacaagaca
4321 ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt
4381 tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
4441 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
4501 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
4561 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
4621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4681 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4801 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
4861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
4921 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
4981 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
5041 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
5101 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
5161 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
5221 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
5281 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
5341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
5401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
5461 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
5521 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
5581 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
5641 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
5701 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
5761 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
5821 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
5881 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
5941 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
6001 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
6061 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
6121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
6181 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
6241 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
6301 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
6361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
6421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
6481 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
6541 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
6601 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
6661 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
6721 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
6781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
6841 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
6901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
6961 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
7021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
7081 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
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pAAVLINK-EF1a-HA-5'-RAI1质粒载体应用举例
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