| 质粒名称: | pAAVLINK-EF1a-HA-5'-POLR3A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001614 |
| 产品用途: | 5' AAVLINK plasmid for POLR3A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | POLR3A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-POLR3A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-POLR3A载体质粒基本信息
pAAVLINK-EF1a-HA-5'-POLR3A质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-POLR3A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-POLR3A质粒载体简介
pAAVLINK-EF1a-HA-5'-POLR3A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-POLR3A 5967 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5967)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5967
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3107
/codon_start=1
/label=POLR3A
/translation="MVKEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQD
NQHAPLLYGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGI
LQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKRGLKKKISDKCRKKNICHHCGAFN
GTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIEHNKEVEPLLGRAQENLNPLV
VLNLFKRIPAEDVPLLLMNPEAGKPSDLILTRLLVPPLCIRPSVVSDLKSGTNEDDLTM
KLTEIIFLNDVIKKHRISGAKTQMIMEDWDFLQLQCALYINSELSGIPLNMAPKKWTRG
FVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKA
NINFLRKLVQNGPEVHPGANFIQQRHTQMKRFLKYGNREKMAQELKYGDIVERHLIDGD
VVLFNRQPSLHKLSIMAHLARVKPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKA
EALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAKACQIIASILVGKD
EKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANLRTKGKQYCGKGEDLCAND
SYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNR
GFSIGIGDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALIL
KELSVIRDHAGSACLRELDKSNSPLTMALCGSK"
misc_feature 1520..1525
/label=SDS
intron 3108..3148
/label=Splicing Donor
misc_feature 3152..3185
/label=lox2272-JT1571
repeat_region 3230..3370
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3230..3359
/label=AAV2 ITR
rep_origin 3445..3900
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4182..4286
/gene="bla"
/label=AmpR promoter
CDS 4287..5147
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5318..5906
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggtgaaggag cagttccggg agacggatgt
781 ggccaagaaa ataagccaca tctgttttgg aatgaagtca cctgaggaga tgcgccagca
841 ggcgcacatc caagttgtga gtaagaacct gtacagccag gacaaccaac atgccccctt
901 gctatatggg gtgctcgacc ataggatggg tacgagtgag aaggatcgtc catgtgaaac
961 ctgtgggaaa aacttggctg actgtctagg ccactatggg tatatcgacc tggagttgcc
1021 gtgttttcat gtagggtact tcagagcagt cataggcatc ttacagatga tctgcaaaac
1081 ctgctgccac atcatgctgt cccaagagga gaagaagcag tttctggact atctaaagag
1141 gcccggcctg acctaccttc agaagcgagg actgaaaaag aaaatctctg acaagtgccg
1201 gaagaaaaac atctgccatc actgtggcgc ttttaatggt accgtaaaga agtgtggact
1261 gctgaaaata attcatgaga aatacaagac caacaaaaaa gtggtggatc ccattgtatc
1321 aaatttcctt cagtcttttg aaacagccat tgaacataat aaagaagtgg agcctctgct
1381 gggaagggca caggaaaact tgaatccctt agtagttctg aatttattta aacgaatccc
1441 agctgaagat gttcctctac ttctgatgaa cccagaagcc ggaaagccgt ctgatttgat
1501 tctcacacga cttttggtgc ctcctttgtg tatcagaccc tccgttgtga gtgatttgaa
1561 gtctggcacc aatgaagatg atctgacaat gaaactgaca gaaatcattt tcctaaacga
1621 tgttattaaa aagcatcgga tctcaggagc caagacccag atgatcatgg aggactggga
1681 tttcctgcag ctgcagtgtg ccctctacat taacagtgag ctctcgggca ttcccctcaa
1741 catggcaccc aagaagtgga ccagaggctt cgtccaacgc ctgaagggaa aacagggtcg
1801 atttagagga aatctctcag gaaagagagt ggatttttct ggcagaacag tcatctcgcc
1861 cgaccccaac ctccggattg atgaggtagc tgtgccagtt catgtggcca aaattctaac
1921 ttttcctgag aaggtaaaca aagcaaacat caatttcttg aggaaactgg ttcaaaacgg
1981 ccctgaggtt cacccaggag caaacttcat tcagcagaga catacgcaga tgaaaaggtt
2041 tttgaaatac ggaaatcgag aaaagatggc tcaagagctc aagtatggtg acatcgtaga
2101 gagacacctc atcgatggag atgtggtgct gttcaatcgg cagccctcgc tgcacaaatt
2161 gagcattatg gctcatctgg ccagggtcaa gccccaccgg accttcagat ttaatgagtg
2221 tgtctgtaca ccctataatg ctgactttga tggtgatgaa atgaaccttc atcttcctca
2281 aacagaagaa gctaaagcag aggcccttgt tctgatgggg actaaagcaa atcttgtaac
2341 cccgaggaat ggggaaccgc tgattgctgc tattcaggat tttctaacag gtgcctatct
2401 cctcactctc aaggacactt tctttgatcg agccaaggct tgccaaatca ttgcttcaat
2461 actggttggc aaggatgaga aaattaaagt tcgcctccca ccgcctacaa tcctaaagcc
2521 tgtcaccctg tggacgggaa agcagatctt cagtgtcatc ctcaggccta gcgatgacaa
2581 tccagtgagg gccaacctgc gaaccaaggg caagcagtac tgtggcaaag gggaagatct
2641 ctgtgccaat gattcctatg ttacaatcca gaacagtgag ttgatgagtg gcagcatgga
2701 caaaggaacc ctagggtcag gatccaagaa caatattttt tacattttgc tgcgagactg
2761 gggacagaat gaagctgcag atgccatgtc acggctcgcc aggctggctc ctgtctacct
2821 gtctaaccgt ggtttctcaa ttgggatcgg tgatgtcaca cctggccaag gactgctgaa
2881 ggccaagtat gagttgctga atgccggcta caagaaatgt gatgagtaca tcgaagccct
2941 gaacacgggc aagctgcagc agcagcctgg ctgcactgct gaggagaccc tggaggcact
3001 gatcctgaag gagctgtctg tgatccgtga ccacgctggc agtgcctgcc tccgggagct
3061 ggacaagagc aacagccccc tcaccatggc tctgtgcggc tccaaaggta agtatcaagg
3121 ttacaagaca ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa
3181 gttattaagt tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
3241 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
3301 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
3361 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
3421 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
3481 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
3541 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
3601 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
3661 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
3721 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
3781 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
3841 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
3901 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
3961 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
4021 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
4081 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
4141 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
4201 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
4261 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
4321 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
4381 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
4441 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
4501 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
4561 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
4621 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
4681 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
4741 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
4801 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
4861 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
4921 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
4981 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
5041 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
5101 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
5161 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
5221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
5281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
5341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
5401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
5461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
5521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
5581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
5641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
5701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
5761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
5821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
5881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
5941 ccttttgctg gccttttgct cacatgt
//
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pAAVLINK-EF1a-HA-5'-POLR3A质粒载体应用举例
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