| 质粒名称: | pAAVLINK-EF1a-HA-5'-POGZ |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001437 |
| 产品用途: | 5' AAVLINK plasmid for POGZ expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | POGZ |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-POGZ载体质粒基本信息
pAAVLINK-EF1a-HA-5'-POGZ质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-POGZ载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-POGZ质粒载体简介
pAAVLINK-EF1a-HA-5'-POGZ质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-POGZ 6413 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6413)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6413
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3575
/codon_start=1
/label=POGZ
/translation="ADTDLFMECEEEELEPWQKISDVIEDSVVEDYNSVDKTTTVSVSQ
QPVSAPVPIAAHASVAGHLSTSTTVSSSGAQNSDSTKKTLVTLIANNNAGNPLVQQGGQ
PLILTQNPAPGLGTMVTQPVLRPVQVMQNANHVTSSPVASQPIFITTQGFPVRNVRPVQ
NAMNQVGIVLNVQQGQTVRPITLVPAPGTQFVKPTVGVPQVFSQMTPVRPGSTMPVRPT
TNTFTTVIPATLTIRSTVPQSQSQQTKSTPSTSTTPTATQPTSLGQLAVQSPGQSNQTT
NPKLAPSFPSPPAVSIASFVTVKRPGVTGENSNEVAKLVNTLNTIPSLGQSPGPVVVSN
NSSAHGSQRTSGPESSMKVTSSIPVFDLQDGGRKICPRCNAQFRVTEALRGHMCYCCPE
MVEYQKKGKSLDSEPSVPSAAKPPSPEKTAPVASTPSSTPIPALSPPTKVPEPNENVGD
AVQTKLIMLVDDFYYGRDGGKVAQLTNFPKVATSFRCPHCTKRLKNNIRFMNHMKHHVE
LDQQNGEVDGHTICQHCYRQFSTPFQLQCHLENVHSPYESTTKCKICEWAFESEPLFLQ
HMKDTHKPGEMPYVCQVCQYRSSLYSEVDVHFRMIHEDTRHLLCPYCLKVFKNGNAFQQ
HYMRHQKRNVYHCNKCRLQFLFAKDKIEHKLQHHKTFRKPKQLEGLKPGTKVTIRASRG
QPRTVPVSSNDTPPSALQEAAPLTSSMDPLPVFLYPPVQRSIQKRAVRKMSVMGRQTCL
ECSFEIPDFPNHFPTYVHCSLCRYSTCCSRAYANHMINNHVPRKSPKYLALFKNSVSGI
KLACTSCTFVTSVGDAMAKHLVFNPSHRSSSILPRGLTWIAHSRHGQTRDRVHDRNVKN
MYPPPSFPTNKAATVKSAGATPAEPEELLTPLAPALPSPASTATPPPTPTHPQALALPP
LATEGAECLNVDDQDE"
misc_feature 765..770
/label=SDS
misc_feature 768..773
/label=SDS
misc_feature 1596..1601
/label=SDS
misc_feature 2871..2876
/label=SDS
misc_feature 2889..2894
/label=SDS
misc_feature 3356..3361
/label=SDS
misc_feature 3359..3364
/label=SDS
intron 3576..3616
/label=Splicing Donor
misc_feature 3620..3653
/label=lox2272-JT1571
repeat_region 3698..3816
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3698..3805
/label=AAV2 ITR
rep_origin 3891..4346
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4628..4732
/gene="bla"
/label=AmpR promoter
CDS 4733..5593
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5764..6352
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgcgg acaccgacct gttcatggaa tgtgaggagg aggagttgga
781 gccatggcag aaaatcagtg atgtcattga ggactctgta gttgaagatt ataattcagt
841 ggataaaact accacagttt ctgtgagcca gcagccagtc tcggctccag tgcccatcgc
901 tgcccatgct tctgttgctg ggcacctctc tacatccacc accgttagta gcagcggggc
961 acagaacagc gacagtacaa agaagactct tgtcacacta attgccaaca acaatgctgg
1021 caatcctttg gtccagcaag gtggacagcc actcatcctg acccagaatc cagccccagg
1081 tctgggcaca atggttactc aaccagtatt gaggcctgtt caggtcatgc agaatgccaa
1141 tcatgtgact agttcccctg tggcctcaca accaatattt atcactacgc agggatttcc
1201 tgtaaggaat gtccggcctg tacaaaatgc aatgaatcag gttgggattg tgctgaacgt
1261 acagcaaggc caaacggtta gaccaattac actagttcca gccccaggta cccagtttgt
1321 taagccgaca gttggagttc cacaagtgtt ctcccagatg acccctgtga ggccaggctc
1381 cacaatgcct gtgaggccca ccaccaacac cttcaccacc gtcatcccgg ccactcttac
1441 cattcgaagc accgtcccac agtcccagtc ccagcagacc aagtccactc ccagcacttc
1501 taccactccc actgccacac agccaacctc actggggcaa ctagctgttc agtctccagg
1561 ccagtcaaac cagaccacga atcccaagct agctccctcc ttcccctctc cacctgcagt
1621 gagcattgcc agctttgtca ctgtgaagcg acctggtgtt acaggcgaaa atagcaatga
1681 agtggccaaa ttggtgaata cccttaacac catcccttcc ctgggccaga gtcctgggcc
1741 agtggtggtg tccaacaaca gctctgctca tggctctcaa agaaccagcg gacctgagtc
1801 ttcaatgaaa gtgacctctt ccatcccagt atttgacctc caggatggtg gacggaaaat
1861 atgtccacga tgtaatgctc aatttcgtgt tactgaagct ttgagaggtc acatgtgtta
1921 ctgttgccca gaaatggttg aataccagaa gaaaggaaag tccctggatt cagaacccag
1981 tgtcccatca gcagcaaagc ccccatcccc tgagaaaaca gctcctgttg cttccacacc
2041 ctcttctaca cctattcctg ctctgtcacc gcctaccaaa gtaccagaac caaatgagaa
2101 cgtgggcgat gccgtccaga ccaaactcat tatgcttgta gatgacttct actatggacg
2161 ggatggtggc aaagtagccc agctcacaaa tttccctaag gtcgccacat ctttccgatg
2221 cccacattgt accaaaaggc taaaaaacaa tattcgattc atgaaccata tgaaacacca
2281 cgtagaactc gatcagcaga acggtgaggt agatggtcac actatctgcc agcactgtta
2341 ccgccagttt tccactccct tccagcttca gtgccacttg gaaaatgttc atagtcccta
2401 tgaatctact accaagtgca agatctgtga atgggcgttt gaaagtgagc cactatttct
2461 ccagcatatg aaggatactc ataagcctgg agagatgcct tatgtttgcc aggtgtgtca
2521 atatcgctcc tcactctact ctgaggtaga tgtccatttt cggatgatcc atgaggatac
2581 ccggcatctg ctctgccctt attgcctgaa ggtcttcaaa aatggcaatg cattccaaca
2641 gcattacatg aggcaccaga agagaaatgt ttatcactgc aacaaatgcc ggctgcagtt
2701 tctctttgcc aaggacaaaa ttgaacacaa gcttcaacac cataaaacct tccgtaaacc
2761 caagcagctg gagggcttga aaccaggcac caaggtgaca atccgggctt cccgagggca
2821 gccacgaact gttcctgtat cctctaatga tacacctccc agcgccttgc aggaggcagc
2881 accgctgacc tcctcaatgg accctctgcc tgtcttcctt tatccccctg tccagcgcag
2941 catccagaag agagctgtta ggaaaatgag tgtcatgggc cggcagacat gcctggagtg
3001 cagcttcgag atcccagact tccctaatca tttccctact tacgtacact gctctctgtg
3061 tcgctatagc acctgctgtt ctcgagctta tgccaaccac atgatcaaca atcatgttcc
3121 acggaagagc cccaagtatt tggctttgtt taaaaattct gtgagtggaa tcaagctggc
3181 ctgcacttca tgtacctttg ttacctctgt gggcgatgct atggccaagc atttggtatt
3241 caacccctct cacagatcca gcagcatcct gccacgggga ctcacttgga tagctcactc
3301 aaggcatggc cagactcgtg accgagtgca tgaccggaac gtgaagaata tgtaccctcc
3361 tccttccttc cccactaaca aagctgccac tgtgaaatct gcgggggcca ccccagctga
3421 gcctgaagag ctactaactc ccttagcccc agcactccca tcaccagcct caactgcaac
3481 cccaccacca acccccactc acccgcaggc tttagccctt ccaccgctgg ctacagaggg
3541 agccgaatgt ctgaatgttg atgatcagga tgaaggtaag tatcaaggtt acaagacagg
3601 tttaaggaga ccaataagct attattcgta taggatactt tatacgaagt tattaagtta
3661 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
3721 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccgg
3781 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
3841 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
3901 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
3961 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4021 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4081 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
4141 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4201 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
4261 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4321 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
4381 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
4441 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
4501 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
4561 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
4621 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
4681 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
4741 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
4801 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
4861 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
4921 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
4981 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
5041 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
5101 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
5161 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
5221 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
5281 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
5341 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
5401 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
5461 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
5521 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
5581 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
5641 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
5701 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
5761 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
5821 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
5881 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
5941 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
6001 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
6061 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
6121 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
6181 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
6241 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
6301 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
6361 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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