| 质粒名称: | pAAVLINK-EF1a-HA-5'-PHRF1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001518 |
| 产品用途: | 5' AAVLINK plasmid for PHRF1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PHRF1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-PHRF1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-PHRF1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-PHRF1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-PHRF1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-PHRF1质粒载体简介
pAAVLINK-EF1a-HA-5'-PHRF1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-PHRF1 6817 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6817)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6817
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3957
/codon_start=1
/label=PHRF1
/translation="DDDSLDELVARSPGPDGHPQVGPADPAGDFEESSVGSSGDSGDDS
DSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDA
ESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGK
ILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVD
EWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERV
RATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKT
RRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLR
RPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSA
KRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLML
GSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNR
PQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTN
KHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRD
AAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPS
SHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENP
SPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQT
VQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAAR
LRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPP
SRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKA
KSRRSSSDRSSSRERAKRKKAKDKS"
misc_feature 921..926
/label=SDS
misc_feature 1266..1271
/label=SDS
misc_feature 1269..1274
/label=SDS
misc_feature 1272..1277
/label=SDS
misc_feature 1386..1391
/label=SDS
misc_feature 1470..1475
/label=SDS
misc_feature 1473..1478
/label=SDS
misc_feature 1616..1621
/label=SDS
misc_feature 2186..2191
/label=SDS
misc_feature 2226..2231
/label=SDS
misc_feature 2275..2280
/label=SDS
misc_feature 3292..3297
/label=SDS
misc_feature 3372..3377
/label=SDS
misc_feature 3561..3566
/label=SDS
misc_feature 3860..3865
/label=SDS
intron 3958..3998
/label=Splicing Donor
misc_feature 4002..4035
/label=lox2272-JT1571
repeat_region 4080..4220
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4080..4209
/label=AAV2 ITR
rep_origin 4295..4750
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5032..5136
/gene="bla"
/label=AmpR promoter
CDS 5137..5997
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6168..6756
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgatg acgacagcct ggatgagctt gtggcccgga gcccagggcc
781 ggatggacac ccacaggtcg gccctgcaga cccggcaggt gactttgaag aaagcagcgt
841 gggcagcagt ggggactctg gggacgacag tgacagcgag catggagatg gcacagacgg
901 agaagacgag ggggcgtctg aggaggaaga cctggaagac agatctggtt ccgaggattc
961 tgaagacgac ggggagacat tgctggaggt agcgggtact caggggaaac tggaagccgc
1021 tggctctttc aattctgatg atgatgcaga gagctgccca atctgtctca acgcattcag
1081 agaccaggcc gtggggacgc cggagaactg tgcccattac ttctgcctgg actgcattgt
1141 cgaatggtcc aagaatgcca attcctgtcc agttgatcga actctattta agtgcatttg
1201 tattcgagct caatttggtg gtaaaatctt aagaaagatc ccagtggaga acaccaaagc
1261 gagcgaggag gaggaggacc cgaccttctg tgaggtgtgc ggcaggagcg accgtgagga
1321 caggcttttg ctctgcgacg gctgtgatgc ggggtaccac atggaatgct tggacccccc
1381 tctccaggag gtgccggtgg acgagtggtt ctgcccggaa tgtgctgcgc ctggtgttgt
1441 ccttgccgct gatgcgggtc ccgtgagtga ggaggaggtc tccctgctct tggctgatgt
1501 ggtgcccacc accagcaggc ttcggcctcg agcaggtagg acccgggcga tagccaggac
1561 acggcagagt gagagagtga gagcaaccgt gaaccggaac cggatctcca cggccaggag
1621 ggtccagcac acaccagggc gcctcgggtc ttccctgctg gatgaagcca tcgaggctgt
1681 ggcgactggc ctgagcactg ccgtgtatca gcgccccctg acgccgcgca ctcccgcccg
1741 acggaagagg aagacaagaa gacggaagaa agtgccggga agaaagaaaa ccccgtccgg
1801 accatccgca aaaagtaaga gctcagcgac aagatctaag aaacgccaac atcgagtgaa
1861 gaagagaaga gggaagaagg taaagagtga agccaccact cgctctcgaa tcgcgcggac
1921 gctgggcctg cgcaggcctg ttcacagcag ctgcatcccg tcagtgttga agccagtgga
1981 gccctctttg gggctgctga gagcggatat tggagctgcc tctctgtctc tgtttggaga
2041 tccctatgag ctggatccct tcgacagcag tgaagagctt tctgcaaacc ctctttcccc
2101 tctgagtgcc aagagacggg ctctgtcccg gtcagccctg cagtcccacc agcccgtggc
2161 caggcccgtc tccgtggggc tttccaggag gcgcctccct gccgcggtgc cagagccaga
2221 cttggaggag gagccagtgc ctgacctgct gggcagcatc ctgtcgggcc agagcctcct
2281 gatgctgggc agcagtgatg tcatcatcca ccgcgacggc tccctcagcg ccaagagggc
2341 ggctccagtt tcttttcagc gaaactcagg cagtctgtcc agaggggaag aaggattcaa
2401 gggctgcctg cagccccgag cactgccctc cgggagcccg gcccaaggcc cgtcaggaaa
2461 caggccacag agcacagggc tcagctgtca aggcaggtcc cgcacccccg cccgcaccgc
2521 gggggcgcct gtgaggctgg acttgccagc agcccctggg gcggttcagg ctcggaactt
2581 gtcaaatggg agtgtgcctg gcttcagaca gagccacagc ccctggttca acggcaccaa
2641 caagcacacc ttgccccttg cctctgccgc gtctaagatc tcaagcagag attctaagcc
2701 cccatgtcgc agtgtggtgc cggggcctcc cctgaagcca gcgcccagaa gaacagacat
2761 ctctgagcta cccaggatac caaagatcag gagagatgac ggtggtggca gacgggatgc
2821 ggccccggcc cacgggcaga gcattgagat ccccagtgcc tgcatcagcc gactgactgg
2881 cagggagggc accgggcagc cagggcgagg cacacgggca gagagcgagg ccagcagcag
2941 ggtgccccgg gagcccgggg tgcacacggg cagctcccgg cccccagccc ccagctccca
3001 tggcagtttg gccccactgg gaccatcaag agggaaaggg gtcgggtcga cctttgagag
3061 cttccggatc aatattcctg gaaacatggc acattccagc cagctctcca gccctggctt
3121 ctgtaacacg ttccggcctg tggacgataa ggagcagagg aaggagaacc cctcacccct
3181 cttctccatc aagaagacga agcagctgcg gagcgaggtc tacgacccat ccgaccccac
3241 cggctccgac tccagcgccc ctggcagcag ccccgagagg tctggccccg gcctcctgcc
3301 ctctgagatc acacgaacca tctccatcaa cagcccgaag gcccagacgg tgcaggctgt
3361 gcgctgcgtc acctcctaca cggtggagag catctttggt acagagcccg aaccccctct
3421 cggaccgtcc tccgccatgt ccaagctccg gggtgcagtg gctgccgagg gggcctctga
3481 cacggagcga gaggagccca cagagagcca gggcctggct gcccggctgc ggaggccatc
3541 ccccccagag ccctgggatg aggaggatgg ggcgtcttgc agcaccttct ttggctctga
3601 ggagcggacg gtgacctgtg tgactgtcgt ggagccggaa gccccaccca gcccggacgt
3661 gctgcaggct gccacccaca gagtcgtgga gctcaggccc ccttcccggt cccgctccac
3721 atccagctcc cgcagcagga agaaggccaa gaggaagagg gtgtccaggg agcacggacg
3781 gacgcgctct gggacgcgct ctgaatccag ggacaggagc tcgaggtcag cgtcaccatc
3841 agtgggtgag gagcgcccca ggaggcagcg gtccaaggcc aagagccggc ggtcctccag
3901 tgaccgctcc agcagccgag agcgagctaa gaggaagaaa gccaaggaca agagcaggta
3961 agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg tataggatac
4021 tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg agcggccgca
4081 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
4141 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
4201 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
4261 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
4321 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
4381 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
4441 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
4501 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
4561 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
4621 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
4681 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
4741 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
4801 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
4861 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
4921 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
4981 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
5041 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
5101 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
5161 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
5221 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
5281 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
5341 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
5401 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
5461 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
5521 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
5581 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
5641 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
5701 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
5761 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
5821 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
5881 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
5941 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
6001 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
6061 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
6121 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
6181 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
6241 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
6301 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
6361 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
6421 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
6481 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
6541 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
6601 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
6661 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
6721 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
6781 cggttcctgg ccttttgctg gccttttgct cacatgt
//
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