| 质粒名称: | pAAVLINK-EF1a-HA-5'-PHIP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001407 |
| 产品用途: | 5' AAVLINK plasmid for PHIP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PHIP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-PHIP载体质粒基本信息
pAAVLINK-EF1a-HA-5'-PHIP质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-PHIP载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-PHIP质粒载体简介
pAAVLINK-EF1a-HA-5'-PHIP质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-PHIP 6811 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6811)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6811
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3951
/codon_start=1
/label=PHIP
/translation="SCERKGLSELRSELYFLIARFLEDGPCQQAAQVLIREVAEKELLP
RRTDWTGKEHPRTYQNLVKYYRHLAPDHLLQICHRLGPLLEQEIPQSVPGVQTLLGAGR
QSLLRTNKSCKHVVWKGSALAALHCGRPPESPVNYGSPPSIADTLFSRKLNGKYRLERL
VPTAVYQHMKMHKRILGHLSSVYCVTFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRG
HAAEISDMAVNYENTMIAAGSCDKMIRVWCLRTCAPLAVLQGHSASITSLQFSPLCSGS
KRYLSSTGADGTICFWLWDAGTLKINPRPAKFTERPRPGVQMICSSFSAGGMFLATGST
DHIIRVYFFGSGQPEKISELEFHTDKVDSIQFSNTSNRFVSGSRDGTARIWQFKRREWK
SILLDMATRPAGQNLQGIEDKITKMKVTMVAWDRHDNTVITAVNNMTLKVWNSYTGQLI
HVLMGHEDEVFVLEPHPFDPRVLFSAGHDGNVIVWDLARGVKIRSYFNMIEGQGHGAVF
DCKCSPDGQHFACTDSHGHLLIFGFGSSSKYDKIADQMFFHSDYRPLIRDANNFVLDEQ
TQQAPHLMPPPFLVDVDGNPHPSRYQRLVPGRENCREEQLIPQMGVTSSGLNQVLSQQA
NQEISPLDSMIQRLQQEQDLRRSGEAGISNTSRLSRGSISSTSEVHSPPNVGLRRSGQI
EGVRQMHSNAPRSEIATERDLVAWSRRVVVPELSAGVASRQEEWRTAKGEEEIKTYRSE
EKRKHLTVPKENKIPTVSKNHAHEHFLDLGESKKQQTNQHNYRTRSALEETPRPSEEIE
NGSSSSDEGEVVAVSGGTSEEEERAWHSDGSSSDYSSDYSDWTADAGINLQPPKKVPKN
KTKKAESSSDEEEESEKQKQKQIKKEKKKVNEEKDGPISPKKKKPKERKQKRLAVGELT
ENGLTLEEWLPSTWITDTIPRRCPFVPQMGDEVYYFRQGHEAYVEMARKNKIYSINPKK
QPWHKMELREQELMKIVGIKYEVGLPTLCCLKLAFLDPDTGKLTGGSFTMKYHDMPDVI
DFLVLRQQFDDAKYRRWNIGDRF"
intron 3952..3992
/label=Splicing Donor
misc_feature 3996..4029
/label=lox2272-JT1571
repeat_region 4074..4214
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4074..4203
/label=AAV2 ITR
rep_origin 4289..4744
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5026..5130
/gene="bla"
/label=AmpR promoter
CDS 5131..5991
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6162..6750
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatcttctt gtgagaggaa aggcctctcg gagctgcgat cggagctcta
781 cttcctcatc gcccggttcc tggaagatgg accctgtcag caggcggctc aggtgctgat
841 ccgcgaggtg gccgagaagg agctgctgcc ccggcgcacc gactggaccg ggaaggagca
901 tcccaggacc taccagaatc tggtgaagta ttacagacac ttagcacctg atcacttgct
961 gcaaatatgt catcgactag gacctcttct tgaacaagaa attcctcaaa gtgttcctgg
1021 agtacaaact ttattaggag ctggaagaca gtctttacta cgcacaaata aaagctgcaa
1081 gcatgttgtg tggaaaggat ctgctctggc tgcgttgcac tgtggaagac cacctgagtc
1141 accagttaac tatggtagcc cacccagcat tgcggatact ctgttttcaa ggaagctgaa
1201 tgggaaatac agacttgagc gacttgttcc aactgcagtg tatcagcaca tgaaaatgca
1261 taaacgaatt cttggacact tgtcatctgt gtactgtgta acttttgatc gaactggcag
1321 acggatattt actggttctg atgactgtct tgtgaaaata tgggcaacag atgatgggag
1381 gttgttagct accttaagag gacatgctgc tgaaatatca gacatggctg taaactatga
1441 gaataccatg atagcagctg gaagttgtga taaaatgatc cgagtctggt gtcttcgaac
1501 ctgtgcacct ttggctgttc ttcagggcca tagtgcatct attacatcac tacagttctc
1561 accattgtgc agtggctcaa agagatatct atcttctact ggggcagatg gcactatttg
1621 tttttggctc tgggatgctg gaacccttaa aataaaccca agacctgcaa aatttacaga
1681 gcgccctcgg cctggagttc aaatgatctg ttcttctttt agtgctggtg gaatgtttct
1741 ggcgacggga agcacagatc atattattcg ggtttatttt tttggatcag gtcagccaga
1801 gaaaatatca gaattggagt ttcatactga caaagttgac agtatccagt tttccaacac
1861 tagtaacagg tttgtaagtg gcagtcgtga tgggacagca cgtatttggc aatttaaacg
1921 aagagagtgg aagagcattt tgttggatat ggctactcgt ccagcaggcc aaaaccttca
1981 aggaatagaa gataaaatca caaaaatgaa ggttactatg gtagcttggg atcgacatga
2041 caatacagtt ataactgcag ttaataacat gactctgaaa gtttggaatt cttacactgg
2101 tcaactaatt catgtcctga tgggtcatga agatgaggta tttgttcttg aaccacaccc
2161 gttcgatcct agagttctct tttctgctgg tcatgatgga aacgtgatag tgtgggatct
2221 ggcaagagga gtcaaaatac gatcttattt caatatgatt gaaggccaag gacatggcgc
2281 agtatttgac tgcaaatgct ctcctgatgg tcagcatttt gcatgcacag actctcatgg
2341 acatctttta atttttggct ttgggtccag tagcaaatat gacaagatag cagatcagat
2401 gttctttcat agtgattatc ggccacttat tcgtgatgcc aacaattttg tattagatga
2461 acagactcag caagcacctc atcttatgcc tccccctttt ttggttgatg ttgatggtaa
2521 ccctcatcca tcaagatatc aaagattagt tcctggccgt gaaaattgca gggaggagca
2581 actcatccct cagatgggag taacttcctc aggactgaat caagttttaa gtcagcaagc
2641 aaaccaggag atcagcccac tggacagcat gattcaaaga ctacaacagg agcaagacct
2701 gagacgttct ggtgaagcag gtatcagtaa taccagccgt ttaagtagag gctccataag
2761 ttctacctca gaggttcatt caccaccaaa cgtaggacta agacgtagtg gacaaattga
2821 aggtgtacgg caaatgcaca gcaacgcacc aagaagtgaa atagccacag agcgggatct
2881 ggtagcttgg agtcgaaggg tggtagtacc cgagctatca gctggtgtag ccagtaggca
2941 agaagaatgg agaactgcaa agggagaaga agaaataaag acttacaggt cagaagagaa
3001 aagaaaacac ttaactgttc caaaagagaa taaaataccc actgtctcaa agaaccatgc
3061 tcatgagcat ttcctggatc ttggagaatc caaaaagcaa cagacaaatc aacacaatta
3121 tcgtacaaga tctgcattgg aagagactcc tagaccctca gaagagatag aaaatggcag
3181 tagttcttca gatgaaggcg aagtagttgc tgtcagtggt ggaacatccg aagaagaaga
3241 gagagcatgg cacagtgatg gcagttctag tgactactcc agtgattact ctgactggac
3301 agcagatgca ggaattaatc tgcagccacc aaagaaagtt cctaagaata aaaccaagaa
3361 agcagaaagc agttcagatg aagaagaaga atctgaaaaa cagaagcaaa aacagattaa
3421 aaaggaaaag aaaaaagtaa atgaagaaaa agatggacca atatcaccaa agaaaaagaa
3481 gcccaaagaa agaaaacaaa agagattggc tgtgggagaa ctaactgaaa atggtttgac
3541 attagaagaa tggttgccat caacatggat tacagatacc attccccgaa gatgtccatt
3601 tgtgccacag atgggtgatg aggtttatta tttccgacaa ggacatgaag cctatgtcga
3661 aatggcccgg aaaaataaaa tatatagtat caatcccaaa aaacaaccat ggcataaaat
3721 ggagctacgg gaacaagaac ttatgaaaat agttggcata aagtatgaag tgggattacc
3781 taccctttgc tgccttaaac ttgcttttct agatcctgat actggtaaac tgactggcgg
3841 atcatttacc atgaaatacc atgatatgcc tgacgttata gatttcctag tcttgagaca
3901 acaatttgat gatgcaaaat acaggcgatg gaatataggt gaccgcttca ggtaagtatc
3961 aaggttacaa gacaggttta aggagaccaa taagctatta ttcgtatagg atactttata
4021 cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc
4081 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
4141 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
4201 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
4261 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
4321 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
4381 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
4441 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
4501 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
4561 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
4621 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
4681 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
4741 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
4801 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
4861 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
4921 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
4981 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
5041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
5101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
5161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
5221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
5281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
5341 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
5401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
5461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
5521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
5581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
5641 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
5701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
5761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
5821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
5881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
5941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
6001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
6061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
6121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
6181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
6241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
6301 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
6361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
6421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
6481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
6541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
6601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
6661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
6721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
6781 ctggcctttt gctggccttt tgctcacatg t
//
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