| 质粒名称: | pAAVLINK-EF1a-HA-5'-NINL |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001458 |
| 产品用途: | 5' AAVLINK plasmid for NINL expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NINL |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-NINL
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-NINL载体质粒基本信息
pAAVLINK-EF1a-HA-5'-NINL质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-NINL载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-NINL质粒载体简介
pAAVLINK-EF1a-HA-5'-NINL质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-NINL 6195 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6195)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6195
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3335
/codon_start=1
/label=NINL
/translation="DEEENHYVSQLREVYSSCDTTGTGFLDRQELTQLCLKLHLEQQLP
VLLQTLLGNDHFARVNFEEFKEGFVAVLSSNAGVRPSDEDSSSLESAASSAIPPKYVNG
SKWYGRRSRPELCDAATEARRVPEQQTQASLKSHLWRSASLESVESPKSDEEAESTKEA
QNELFEAQGQLQTWDSEDFGSPQKSCSPSFDTPESQIRGVWEELGVGSSGHLSEQELAV
VCQSVGLQGLEKEELEDLFNKLDQDGDGKVSLEEFQLGLFSHEPALLLESSTRVKPSKA
WSHYQVPEESGCHTTTTSSLVSLCSSLRLFSSIDDGSGFAFPDQVLAMWTQEGIQNGRE
ILQSLDFSVDEKVNLLELTWALDNELMTVDSAVQQAALACYHQELSYQQGQVEQLARER
DKARQDLERAEKRNLEFVKEMDDCHSTLEQLTEKKIKHLEQGYRERLSLLRSEVEAERE
LFWEQAHRQRAALEWDVGRLQAEEAGLREKLTLALKENSRLQKEIVEVVEKLSDSERLA
LKLQKDLEFVLKDKLEPQSAELLAQEERFAAVLKEYELKCRDLQDRNDELQAELEGLWA
RLPKNRHSPSWSPDGRRRQLPGLGPAGISFLGNSAPVSIETELMMEQVKEHYQDLRTQL
ETKVNYYEREIAALKRNFEKERKDMEQARRREVSVLEGQKADLEELHEKSQEVIWGLQE
QLQDTARGPEPEQMGLAPCCTQALCGLALRHHSHLQQIRREAEAELSGELSGLGALPAR
RDLTLELEEPPQGPLPRGSQRSEQLELERALKLQPCASEKRAQMCVSLALEEEELELAR
GKRVDGPSLEAEMQALPKDGLVAGSGQEGTRGLLPLRPGCGERPLAWLAP"
misc_feature 886..891
/label=SDS
misc_feature 1320..1325
/label=SDS
misc_feature 1743..1748
/label=SDS
misc_feature 2089..2094
/label=SDS
misc_feature 2190..2195
/label=SDS
misc_feature 2373..2378
/label=SDS
misc_feature 2805..2810
/label=SDS
misc_feature 3159..3164
/label=SDS
misc_feature 3162..3167
/label=SDS
misc_feature 3264..3269
/label=SDS
misc_feature 3280..3285
/label=SDS
intron 3336..3376
/label=Splicing Donor
misc_feature 3380..3413
/label=lox2272-JT1571
repeat_region 3458..3598
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3458..3587
/label=AAV2 ITR
rep_origin 3673..4128
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4410..4514
/gene="bla"
/label=AmpR promoter
CDS 4515..5375
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5546..6134
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctga tgaagaagag aaccactatg tctcgcagct
781 cagggaagtc tacagcagct gcgacaccac ggggactggc tttctggacc gccaggagct
841 gacccagctc tgccttaagc ttcacctgga gcagcagctg cccgtcctcc tgcagacgct
901 tctcggaaac gaccatttcg ccagggttaa ctttgaggaa tttaaggaag gttttgtggc
961 tgtgttgtct tcaaatgctg gtgttcgccc ctcagatgaa gacagtagtt ctttggaatc
1021 agctgcctcc agtgccatcc ctccaaagta tgtgaatggt tctaagtggt atggccgtcg
1081 gagccggcct gagctatgtg acgctgccac agaagccaga cgcgtgccgg agcagcaaac
1141 ccaggccagc ctgaaaagtc acctctggcg ctcagcgtct ctggagagcg tggagagtcc
1201 caagtcagat gaagaggccg agagcactaa agaagctcag aatgaattat ttgaagcaca
1261 aggacagctg cagacctggg attctgagga ctttgggagc ccccagaagt cctgcagccc
1321 ctcctttgac accccagaga gccagatccg gggcgtgtgg gaagagctgg gggtgggcag
1381 cagcggacac ctgagcgagc aggagctggc tgtggtctgc cagagcgtcg ggctccaggg
1441 actcgagaaa gaggaactcg aagacctgtt taacaaactg gatcaagacg gagacggcaa
1501 agtgagtctt gaggaattcc agcttggcct cttcagtcat gagcccgcgc tacttctaga
1561 gtcttccact cgggttaaac cgagcaaggc ttggtctcat taccaggtcc cagaggagag
1621 cggctgccac accaccacaa cctcatccct cgtgtccctg tgctccagcc tgcgcctctt
1681 ctccagcatt gacgatggtt ctggcttcgc ttttcctgat caggtcctgg ccatgtggac
1741 ccaggagggg attcagaatg gcagggagat cttgcagagc ctggacttca gcgtggacga
1801 gaaggtgaac cttctggagc tgacctgggc ccttgacaac gagctcatga cagtggacag
1861 tgccgtccag caggcagccc tggcctgcta ccaccaggag ctgagctacc agcaagggca
1921 ggtggagcag ctggcaaggg agcgtgacaa ggcaaggcag gacctggaga gggccgagaa
1981 gaggaacctg gagtttgtga aagagatgga cgactgccac tccaccctgg agcagctcac
2041 ggagaagaaa atcaagcatc tggagcaggg gtaccgggaa aggctgagcc tcctgcggtc
2101 tgaggtggag gcggagcgag agctgttctg ggagcaggcc cacaggcaga gggccgcgct
2161 ggagtgggac gtggggcgcc tgcaggctga ggaggctggc ctccgcgaga agctgaccct
2221 ggccctgaag gaaaacagtc gcctacagaa ggagattgtg gaagtggtgg aaaagctttc
2281 ggattcggag aggctggccc tgaagctgca gaaggacctg gagtttgtgc tgaaggacaa
2341 gctggagcca cagagtgcag agctcctggc ccaggaggag cggttcgcag cagtcctgaa
2401 ggaatacgag ctcaagtgcc gggacctgca ggaccgcaac gatgagctgc aagctgagct
2461 ggaaggcctg tgggcgcggc tgcccaagaa ccggcacagc ccctcatgga gcccggatgg
2521 gcgcagacgg cagctccctg gactcggccc agcaggcatt tcattcctgg gtaattctgc
2581 tccagtgagt atagaaacgg agctgatgat ggagcaggta aaggagcatt accaagacct
2641 caggacccag ctggagacca aggtaaatta ctacgaaagg gaaattgcgg cactgaaaag
2701 gaactttgag aaggagagga aggacatgga gcaggctcgc cggcgcgagg tcagcgtgct
2761 ggagggtcag aaggccgacc tggaggagct ccacgagaag tctcaggagg tcatctgggg
2821 cctgcaggag cagctgcagg acacagcccg cggccccgag cctgagcaga tgggcctggc
2881 accctgctgc acccaggcac tgtgtggcct ggccctgcgg catcacagcc acctgcagca
2941 gatcaggaga gaggctgagg cggagctgag tggagagctg tcggggctgg gagccctgcc
3001 cgctcgcaga gacctgacct tggagctgga ggagccgccg cagggacccc tgccacgcgg
3061 gagccagagg tcggagcagc tggagctgga gagggcactg aagctgcagc cctgtgcgag
3121 cgagaagcgc gcccagatgt gcgtatcgtt ggccctcgag gaggaggagt tggagcttgc
3181 ccgcgggaag cgagtggacg ggccctccct ggaagcagag atgcaggccc tgccgaaaga
3241 tgggctggtg gcaggaagtg gccaggaggg cacacgtggc ctcctaccac tgcgtccggg
3301 ctgtggggag cggccactgg cctggctggc cccaggtaag tatcaaggtt acaagacagg
3361 tttaaggaga ccaataagct attattcgta taggatactt tatacgaagt tattaagtta
3421 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
3481 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
3541 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
3601 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
3661 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
3721 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
3781 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
3841 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
3901 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
3961 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
4021 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
4081 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
4141 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
4201 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
4261 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
4321 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
4381 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
4441 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
4501 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
4561 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
4621 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
4681 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
4741 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
4801 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
4861 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
4921 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
4981 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
5041 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
5101 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
5161 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
5221 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
5281 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
5341 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
5401 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
5461 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
5521 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
5581 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
5641 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
5701 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
5761 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
5821 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
5881 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
5941 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
6001 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
6061 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
6121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
6181 cttttgctca catgt
//
