| 质粒名称: | pAAVLINK-EF1a-HA-5'-MYO5C |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001545 |
| 产品用途: | 5' AAVLINK plasmid for MYO5C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MYO5C |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-MYO5C载体质粒基本信息
pAAVLINK-EF1a-HA-5'-MYO5C质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-MYO5C载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-MYO5C质粒载体简介
pAAVLINK-EF1a-HA-5'-MYO5C质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-MYO5C 6806 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6806)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6806
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3946
/codon_start=1
/label=MYO5C
/translation="AVAELYTQYNRVWIPDPEEVWKSAEIAKDYRVGDKVLRLLLEDGT
ELDYSVNPESLPPLRNPDILVGENDLTALSYLHEPAVLHNLRIRFAESKLIYTYSGIIL
VAMNPYKQLPIYGDAIIHAYSGQNMGDMDPHIFAVAEEAYKQMARNNRNQSIIVSGESG
AGKTVSARYAMRYFATVSKSGSNAHVEDKVLASNPITEAVGNAKTTRNDNSSRFGKYTE
ISFDEQNQIIGANMSTYLLEKSRVVFQSENERNYHIFYQLCASAQQSEFKHLKLGSAEE
FNYTRMGGNTVIEGVNDRAEMVETQKTFTLLGFKEDFQMDVFKILAAILHLGNVQITAV
GNERSSVSEDDSHLKVFCELLGLESGRVAQWLCNRKIVTSSETVVKPMTRPQAVNARDA
LAKKIYAHLFDFIVERINQALQFSGKQHTFIGVLDIYGFETFDVNSFEQFCINYANEKL
QQQFNMHVFKLEQEEYMKEDIPWTLIDFYDNQPVIDLIEAKMGILELLDEECLLPHGTD
ENWLQKLYNNFVNRNPLFEKPRMSNTSFVIQHFADKVEYKCEGFLEKNRDTVYDMLVEI
LRASKFHLCANFFQENPTPPSPFGSMITVKSAKQVIKPNSKHFRTTVGSKFRSSLYLLM
ETLNATTPHYVRCIKPNDEKLPFEFDSKRIVQQLRACGVLETIRISAQSYPSRWTYIEF
YSRYGILMTKQELSFSDKKEVCKVVLHRLIQDSNQYQFGKTKIFFRAGQVAYLEKLRLD
KLRQSCVMVQKHMRGWLQRKKFLRERRAALIIQQYFRGQQTVRKAITAVALKEAWAAII
IQKHCRGYLVRSLYQLIRMATITMQAYSRGFLARRRYRKMLEEHKAVILQKYARAWLAR
RRFQSIRRFVLNIQLTYRVQRLQKKLEDQNKENHGLVEKLTSLAALRAGDVEKIQKLEA
ELEKAATHRRNYEEKGKRYRDAVEEKLAKLQKHNSELETQKEQIQLKLQEKTEELKEKM
DNLTKQLFDDVQKEERQRMLLEKSFELKTQDYEKQIQSLKEEIKALKDEKMQLQHLVEG
EHVTSDGLKAEVARLSKQ"
misc_feature 1465..1470
/label=SDS
misc_feature 1692..1697
/label=SDS
misc_feature 2531..2536
/label=SDS
misc_feature 2886..2891
/label=SDS
misc_feature 3284..3289
/label=SDS
misc_feature 3287..3292
/label=SDS
intron 3947..3987
/label=Splicing Donor
misc_feature 3991..4024
/label=lox2272-JT1571
repeat_region 4069..4209
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4069..4198
/label=AAV2 ITR
rep_origin 4284..4739
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5021..5125
/gene="bla"
/label=AmpR promoter
CDS 5126..5986
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6157..6745
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctgc ggtggccgag ctgtacacgc agtacaacag
781 ggtctggatt cccgatcctg aagaagtttg gaagtctgct gaaatagcca aggactacag
841 agttggtgac aaggtcctgc gactcctgct ggaggatgga acggagctgg attattctgt
901 caatccagaa tctctgcctc cacttcggaa tcctgacatc ctcgtgggcg agaatgacct
961 cacggctctc agctatcttc acgagcccgc ggtgctccac aacctcagaa tccgctttgc
1021 agaatccaaa ctcatttaca cctacagtgg aatcattttg gtggccatga atccttacaa
1081 gcagttgcca atatacggag atgccatcat ccacgcctac agcgggcaga acatgggcga
1141 tatggaccca cacatatttg ccgtggcaga agaggcatac aagcagatgg ccagaaacaa
1201 cagaaaccag tccataattg taagtgggga gtcaggtgct ggaaagacag tgtcggctcg
1261 ctatgccatg aggtactttg ccaccgtcag caaatcgggc agcaacgctc acgtggaaga
1321 caaggtcctg gcatccaatc ccatcaccga ggccgttgga aatgccaaga ccacccgcaa
1381 tgacaatagt agtcggtttg ggaaatacac agaaatcagt tttgatgaac aaaatcaaat
1441 tataggagcc aacatgagca cttacctcct ggagaaatcc agagttgtct ttcaatcgga
1501 aaatgaacga aattaccaca ttttctatca gctttgtgca tctgcacagc agtcggaatt
1561 taaacatctt aaattgggga gtgccgaaga atttaattat acaagaatgg gaggcaatac
1621 tgtcattgag ggtgtgaatg atcgagctga aatggtagag actcaaaaga ccttcacgct
1681 tctgggtttc aaggaggatt ttcagatgga cgtttttaaa atcctggcag ccatcctaca
1741 tctgggcaac gtgcagatca ccgcggtggg caacgagagg tcctcagtta gtgaggatga
1801 cagtcacctg aaggtgttct gtgagctcct gggcctggag agtggcagag ttgctcagtg
1861 gctgtgcaat cgcaaaatcg tcacaagctc tgagacggtg gtaaaaccca tgaccaggcc
1921 tcaggctgtc aacgccaggg atgcactggc caaaaagatc tatgctcacc tgttcgactt
1981 cattgtggag agaattaacc aagcgttgca gttttcaggc aagcagcaca cttttattgg
2041 tgttttggac atttatggtt ttgaaacctt tgatgtgaac agctttgaac aattttgcat
2101 caattacgct aatgaaaaac tgcaacaaca gtttaacatg catgtcttca aactggaaca
2161 agaagaatac atgaaggaag atataccttg gacgctgata gatttttatg acaatcaacc
2221 agttattgac ctgattgaag caaaaatggg aattctggag ttactggatg aagaatgttt
2281 gttaccacat ggaactgatg aaaactggct tcaaaagctg tataataatt ttgtcaacag
2341 gaaccctttg tttgaaaagc ctagaatgtc aaacacatcc tttgtcatcc agcactttgc
2401 tgataaggta gagtataaat gtgaaggttt cctggagaaa aacagagaca ccgtctatga
2461 catgctggtt gaaatcctga gagcaagcaa gtttcatctc tgtgccaact tttttcaaga
2521 aaatccaact cctccttctc cttttggttc aatgattaca gttaaatctg caaagcaagt
2581 catcaagcca aacagcaagc atttccgaac cacagttggg agcaagttcc gcagctctct
2641 gtacttgctc atggagaccc tcaatgcgac gacgccccac tacgttcgat gcatcaagcc
2701 aaatgatgag aagttaccct ttgaatttga ctccaaaaga attgttcagc agctgcgagc
2761 ctgcggcgtt ttagaaacga ttcgcattag tgcacagagc tacccttcca ggtggacata
2821 catcgagttc tacagtcgct acggcattct catgaccaag caagagcttt ccttcagcga
2881 taaaaaggag gtgtgcaagg tggttttaca cagactcatc caggattcta atcagtacca
2941 gtttggtaaa accaaaattt tcttcagagc aggacaagtg gcttatttag agaaacttcg
3001 attggataaa ctgaggcaga gttgtgttat ggtacaaaag cacatgcgtg gctggctcca
3061 gaggaaaaaa ttcctccgag agagacgagc cgccctgata atccagcagt acttccgggg
3121 tcagcaaact gtgaggaaag ctattactgc agtggcctta aaagaagctt gggcagccat
3181 aatcattcag aagcactgcc gtgggtatct tgttcgcagc ctgtatcagt tgattcgcat
3241 ggccaccatc acaatgcagg cctacagccg aggattcctg gcaaggagga ggtatcgaaa
3301 gatgctggag gaacataagg ctgtgatcct acagaaatac gcacgggcgt ggctggccag
3361 acgcagattc cagagtatcc gacgattcgt gcttaatatt cagcttactt acagggtcca
3421 gcgtttgcag aaaaagctgg aagatcagaa caaagaaaac catgggctgg tggagaagct
3481 gactagcctg gctgctcttc gagctgggga tgtggaaaag attcagaagc tggaagcaga
3541 actagaaaaa gcagccactc acaggcgaaa ttacgaggag aaggggaaga gatacaggga
3601 tgctgtggaa gagaaattgg caaagcttca gaagcataat tcagaactgg aaacacagaa
3661 agaacaaata cagctgaagc ttcaagagaa gactgaagag ttaaaagaaa aaatggacaa
3721 cctcaccaag cagctctttg atgatgtaca aaaggaagaa cggcaaagaa tgcttcttga
3781 aaaaagtttt gaactgaaaa cacaagacta tgagaagcag attcagtctt tgaaagaaga
3841 aattaaagct ctcaaggatg agaagatgca actccaacac ctggtggagg gggagcacgt
3901 cacttctgat ggcttgaagg cggaagtggc ccgcctgagc aagcaggtaa gtatcaaggt
3961 tacaagacag gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag
4021 ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
4081 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
4141 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
4201 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
4261 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
4321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
4381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
4441 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
4501 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
4561 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
4621 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
4681 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
4741 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
4801 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
4861 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
4921 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
4981 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
5041 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
5101 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
5161 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
5221 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
5281 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
5341 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
5401 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
5461 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
5521 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
5581 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
5641 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
5701 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
5761 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
5821 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
5881 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
5941 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
6001 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
6061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
6121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
6181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
6241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
6301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
6361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
6421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
6481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
6541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
6601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
6661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
6721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
6781 cttttgctgg ccttttgctc acatgt
//
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pAAVLINK-EF1a-HA-5'-MYO5C质粒载体应用举例
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