| 质粒名称: | pAAVLINK-EF1a-HA-5'-KIF14 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001515 |
| 产品用途: | 5' AAVLINK plasmid for KIF14 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
EF1a
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
KIF14
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-KIF14载体质粒基本信息
pAAVLINK-EF1a-HA-5'-KIF14质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-KIF14载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-KIF14质粒载体简介
pAAVLINK-EF1a-HA-5'-KIF14质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-KIF14 6665 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6665)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6665
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3805
/codon_start=1
/label=KIF14
/translation="SLHSTHNRNNSGDILDIPSSQNSSSLNALTHSSRLKLHLKSDMSE
CENDDPLLRSAGKVRDINRTYVISASRKTADMPLTPNPVGRLALQRRTTRNKESSLLVS
ELEDTTEKTAETRLTLQRRAKTDSAEKWKTAEIDSVKMTLNVGGETENNGVSKESRTNV
RIVNNAKNSFVASSVPLDEDPQVIEMMADKKYKETFSAPSRANENVALKYSSNRPPIAS
LSQTEVVRSGHLTTKPTQSKLDIKVLGTGNLYHRSIGKEIAKTSNKFGSLEKRTPTKCT
TEHKLTTKCSLPQLKSPAPSILKNRMSNLQVKQRPKSSFLANKQERSAENTILPEEETV
VQNTSAGKDPLKVENSQVTVAVRVRPFTKREKIEKASQVVFMSGKEITVEHPDTKQVYN
FIYDVSFWSFDECHPHYASQTTVYEKLAAPLLERAFEGFNTCLFAYGQTGSGKSYTMMG
FSEEPGIIPRFCEDLFSQVARKQTQEVSYHIEMSFFEVYNEKIHDLLVCKDENGQRKQP
LRVREHPVYGPYVEALSMNIVSSYADIQSWLELGNKQRATAATGMNDKSSRSHSVFTLV
MTQTKTEFVEGEEHDHRITSRINLIDLAGSERCSTAHTNGDRLKEGVSINKSLLTLGKV
ISALSEQANQRSVFIPYRESVLTWLLKESLGGNSKTAMIATISPAASNIEETLSTLRYA
NQARLIVNIAKVNEDMNAKLIRELKAEIAKLKAAQRNSRNIDPERYRLCRQEITSLRMK
LHQQERDMAEMQRVWKEKFEQAEKRKLQETKELQKAGIMFQMDNHLPNLVNLNEDPQLS
EMLLYMIKEGTTTVGKYKPNSSHDIQLSGVLIADDHCTIKNFGGTVSIIPVGEAKTYVN
GKHILEITVLRHGDRVILGGDHYFRFNHPVEVQKGKRPSGRDTPISEGPKDFEFAKNEL
LMAQRSQLEAEIKEAQLKAKEEMMQGIQIAKEMAQQELSSQKAAYESKIKALEAELREE
SQRKKMQEINNQKANHKIEELEKAKQHLEQ"
CDS 2899..2910
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
intron 3806..3846
/label=Splicing Donor
misc_feature 3850..3883
/label=lox2272-JT1571
repeat_region 3928..4068
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3928..4057
/label=AAV2 ITR
rep_origin 4143..4598
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4880..4984
/gene="bla"
/label=AmpR promoter
CDS 4985..5845
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6016..6604
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatcttc attacacagt actcataata gaaataacag
781 cggtgatatt cttgatattc cttcttccca aaatagttca tcactgaatg ccctcaccca
841 cagtagccga cttaagctgc atttgaagtc ggatatgtca gaatgtgaaa atgatgatcc
901 attattgaga tctgcaggta aagtcagaga cataaataga acttatgtta tttctgccag
961 tagaaaaaca gcagacatgc cccttacccc taatcctgta ggtagattgg cacttcagag
1021 gagaactaca aggaacaaag aatcatcttt gcttgttagt gagttggaag acacaactga
1081 aaaaacagca gaaacacgtc ttacattaca acgtcgtgct aaaacagatt ctgcagaaaa
1141 gtggaaaaca gctgaaatag attctgtcaa aatgacactg aatgtgggag gtgaaacaga
1201 aaataatggt gtttctaagg aaagtagaac aaatgtaagg attgtaaata atgctaaaaa
1261 ctcttttgtt gcctcttctg tacctttaga tgaagatcca caggtcattg aaatgatggc
1321 tgataagaaa tacaaagaaa cattttctgc ccccagtaga gcaaatgaaa atgttgcact
1381 taagtactca agtaatagac cacccattgc ttccctgagt cagactgaag ttgttagatc
1441 aggacacttg acaacgaaac ctactcagag caagttggat atcaaagtgt tgggaacagg
1501 aaacttgtat catagaagta ttgggaagga aattgcaaaa acttcaaata aatttgggag
1561 cttagaaaaa agaacaccta caaaatgtac aacagaacac aaactgacaa caaagtgcag
1621 cctgcctcag cttaagagcc cagctccatc aatactgaag aatagaatgt ctaaccttca
1681 agttaaacaa agaccaaaaa gttcctttct tgcaaataaa caggaaagat ccgcagaaaa
1741 tacaattctt cccgaagaag aaactgtagt tcagaacacc tctgcaggaa aagacccctt
1801 aaaagtagag aatagtcaag tgacagtggc agtacgcgta agacctttca ccaagagaga
1861 gaagattgaa aaagcatccc aggtagtctt catgagtggg aaagaaataa ctgtggaaca
1921 ccctgacacg aaacaagttt ataattttat ttatgatgtt tcattctggt cttttgatga
1981 atgtcatcct cactacgcta gccagacaac tgtctatgag aagctagcag caccactcct
2041 agaaagagcc ttcgaaggct tcaatacctg tctttttgct tatggtcaga ctggctctgg
2101 aaaatcatat acgatgatgg gatttagtga agaaccagga ataattccaa gattttgtga
2161 agatcttttt tctcaagtag ccagaaaaca aacccaagag gtcagctatc acattgaaat
2221 gagcttcttt gaagtatata atgaaaaaat tcacgacctt ctggtttgta aagatgaaaa
2281 tgggcagaga aagcaaccac tgagagtgag ggaacatcct gtttatggac catatgttga
2341 agcactgtca atgaacattg tcagttctta cgctgatatc cagagttggc tagaattggg
2401 aaataaacaa agagctactg ctgctactgg tatgaatgat aaaagttccc gatctcattc
2461 agttttcacc ctggtgatga cccagaccaa gacagaattt gtggaagggg aagaacacga
2521 tcacagaata acaagtcgaa ttaacctaat agatctggca ggcagtgagc gctgctctac
2581 ggctcacact aatggagatc gactaaagga aggtgtgagt attaataagt ccttgctaac
2641 tttgggaaaa gttatatctg cactttcgga acaagcaaac caaaggagtg tttttattcc
2701 ttatcgtgaa tctgttctta catggctgtt aaaagaaagt ctgggtggaa attcaaaaac
2761 tgcaatgatt gctacgatta gtcccgctgc cagcaacata gaagaaacat taagcacact
2821 tagatatgct aaccaagccc gtttaatagt caacattgct aaagtaaatg aagatatgaa
2881 cgctaagtta attagagaat tgaaggcaga aattgcaaag ctaaaagctg ctcagagaaa
2941 cagtcggaat attgaccctg aacgatacag gctctgtcgg caagaaataa catccttaag
3001 aatgaaactg catcaacagg agagagacat ggcagaaatg caaagagtgt ggaaagaaaa
3061 gtttgaacaa gctgaaaaaa gaaaacttca agaaacaaaa gagttacaga aagcaggaat
3121 tatgtttcaa atggacaatc atttaccaaa ccttgttaat ctgaatgaag atccacaact
3181 atctgagatg ctgctatata tgataaaaga aggaacaact acagttggaa agtataaacc
3241 aaactcaagc catgatattc agttatctgg ggtgctgatt gctgatgatc attgtactat
3301 caaaaatttt ggtgggacag tgagtattat cccagttggg gaagcaaaga catatgtaaa
3361 tggaaaacat attttggaaa tcacagtatt acgtcatggt gatcgagtga ttcttggtgg
3421 agatcattat tttagattta atcatccagt agaagtccag aaaggaaaaa ggccatctgg
3481 aagagatact cctataagtg agggtccaaa agactttgaa tttgcaaaaa atgagttgct
3541 catggcacag agatcacaac ttgaagcaga aataaaagag gctcagttga aggcaaagga
3601 agaaatgatg caaggaatcc agattgcaaa agaaatggct cagcaagagc tttcttctca
3661 aaaagctgca tatgaaagca aaataaaagc actggaagca gaactgagag aagagtctca
3721 aaggaaaaaa atgcaggaaa taaataacca gaaggctaat cacaaaattg aggaattaga
3781 aaaggcaaag cagcatcttg aacaggtaag tatcaaggtt acaagacagg tttaaggaga
3841 ccaataagct attattcgta taggatactt tatacgaagt tattaagtta tggagaccgg
3901 tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
3961 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
4021 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
4081 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
4141 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
4201 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
4261 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
4321 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
4381 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
4441 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
4501 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
4561 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
4621 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
4681 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
4741 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
4801 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
4861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
4921 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
4981 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
5041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
5101 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
5161 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
5221 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
5281 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
5341 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
5401 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
5461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
5521 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
5581 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
5641 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
5701 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
5761 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
5821 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
5881 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
5941 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
6001 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
6061 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
6121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
6181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
6241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
6301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
6361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
6421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
6481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
6541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
6601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
6661 catgt
//
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