| 质粒名称: |
pAAVLINK-EF1a-HA-5'-ITSN1
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001542 |
| 产品用途: | 5' AAVLINK plasmid for ITSN1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ITSN1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-ITSN1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-ITSN1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-ITSN1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-ITSN1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-ITSN1质粒载体简介
pAAVLINK-EF1a-HA-5'-ITSN1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-ITSN1 7093 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7093)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7093
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 169..711
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 198..409
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 422..690
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 516..521
/label=SDS
CDS 727..753
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 760..4233
/codon_start=1
/label=ITSN1
/translation="AQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARN
FFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQ
PVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPP
VIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRL
KYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILA
MHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQ
LEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQE
QERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLN
QRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRE
LRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKEL
ARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQE
RKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEE
KGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESR
SHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPV
KPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDA
WAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNH
LNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASL
KRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGV
FPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNP
GGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQ"
misc_feature 2027..2032
/label=SDS
misc_feature 2035..2040
/label=SDS
misc_feature 2753..2758
/label=SDS
intron 4234..4274
/label=Splicing Donor
misc_feature 4278..4311
/label=lox2272-JT1571
repeat_region 4356..4496
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4356..4485
/label=AAV2 ITR
rep_origin 4571..5026
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5308..5412
/gene="bla"
/label=AmpR promoter
CDS 5413..6273
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6444..7032
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
721 accatgtacc cttatgacgt acctgactat gctggatctg ctcagtttcc aacacctttt
781 ggtggcagcc tggatatctg ggccataact gtagaggaaa gagcgaagca tgatcagcag
841 ttccatagtt taaagccaat atctggattc attactggtg atcaagctag aaactttttt
901 tttcaatctg ggttacctca acctgtttta gcacagatat gggcactagc tgacatgaat
961 aatgatggaa gaatggatca agtggagttt tccatagcta tgaaacttat caaactgaag
1021 ctacaaggat atcagctacc ctctgcactt ccccctgtca tgaaacagca accagttgct
1081 atttctagcg caccagcatt tggtatggga ggtatcgcca gcatgccacc gcttacagct
1141 gttgctccag tgccaatggg atccattcca gttgttggaa tgtctccaac cctagtatct
1201 tctgttccca cagcagctgt gccccccctg gctaacgggg ctccccctgt tatacaacct
1261 ctgcctgcat ttgctcatcc tgcagccaca ttgccaaaga gttcttcctt tagtagatct
1321 ggtccagggt cacaactaaa cactaaatta caaaaggcac agtcatttga tgtggccagt
1381 gtcccaccag tggcagagtg ggctgttcct cagtcatcaa gactgaaata caggcaatta
1441 ttcaatagtc atgacaaaac tatgagtgga cacttaacag gtccccaagc aagaactatt
1501 cttatgcagt caagtttacc acaggctcag ctggcttcaa tatggaatct ttctgacatt
1561 gatcaagatg gaaaacttac agcagaggaa tttatcctgg caatgcacct cattgatgta
1621 gctatgtctg gccaaccact gccacctgtc ctgcctccag aatacattcc accttctttt
1681 agaagagttc gatctggcag tggtatatct gtcataagct caacatctgt agatcagagg
1741 ctaccagagg aaccagtttt agaagatgaa caacaacaat tagaaaagaa attacctgta
1801 acgtttgaag ataagaagcg ggagaacttt gaacgtggca acctggaact ggagaaacga
1861 aggcaagctc tcctggaaca gcagcgcaag gagcaggagc gcctggccca gctggagcgg
1921 gcggagcagg agaggaagga gcgtgagcgc caggagcaag agcgcaaaag acaactggaa
1981 ctggagaagc aactggaaaa gcagcgggag ctagaacggc agagagagga ggagaggagg
2041 aaagaaattg agaggcgaga ggctgcaaaa cgggaacttg aaaggcaacg acaacttgag
2101 tgggaacgga atcgaaggca agaactacta aatcaaagaa acaaagaaca agaggacata
2161 gttgtactga aagcaaagaa aaagactttg gaatttgaat tagaagctct aaatgataaa
2221 aagcatcaac tagaagggaa acttcaagat atcagatgtc gattgaccac ccaaaggcaa
2281 gaaattgaga gcacaaacaa atctagagag ttgagaattg ccgaaatcac ccatctacag
2341 caacaattac aggaatctca gcaaatgctt ggaagactta ttccagaaaa acagatactc
2401 aatgaccaat taaaacaagt tcagcagaac agtttgcaca gagattcact tgttacactt
2461 aaaagagcct tagaagcaaa agaactagct cggcagcacc tacgagacca actggatgaa
2521 gtggagaaag aaactagatc aaaactacag gagattgata ttttcaataa tcagctgaag
2581 gaactaagag aaatacacaa taagcaacaa ctccagaagc aaaagtccat ggaggctgaa
2641 cgactgaaac agaaagaaca agaacgaaag atcatagaat tagaaaaaca aaaagaagaa
2701 gcccaaagac gagctcagga aagggacaag cagtggctgg agcatgtgca gcaggaggac
2761 gagcatcaga gaccaagaaa actccacgaa gaggaaaaac tgaaaaggga ggagagtgtc
2821 aaaaagaagg atggcgagga aaaaggcaaa caggaagcac aagacaagct gggtcggctt
2881 ttccatcaac accaagaacc agctaagcca gctgtccagg caccctggtc cactgcagaa
2941 aaaggtccac ttaccatttc tgcacaggaa aatgtaaaag tggtgtatta ccgggcactg
3001 tacccctttg aatccagaag ccatgatgaa atcactatcc agccaggaga catagtcatg
3061 gttaaagggg aatgggtgga tgaaagccaa actggagaac ccggctggct tggaggagaa
3121 ttaaaaggaa agacagggtg gttccctgca aactatgcag agaaaatccc agaaaatgag
3181 gttcccgctc cagtgaaacc agtgactgat tcaacatctg cccctgcccc caaactggcc
3241 ttgcgtgaga cccccgcccc tttggcagta acctcttcag agccctccac gacccctaat
3301 aactgggccg acttcagctc cacgtggccc accagcacga atgagaaacc agaaacggat
3361 aactgggatg catgggcagc ccagccctct ctcaccgttc caagtgccgg ccagttaagg
3421 cagaggtccg cctttactcc agccacggcc actggctcct ccccgtctcc tgtgctaggc
3481 cagggtgaaa aggtggaggg gctacaagct caagccctat atccttggag agccaaaaaa
3541 gacaaccact taaattttaa caaaaatgat gtcatcaccg tcctggaaca gcaagacatg
3601 tggtggtttg gagaagttca aggtcagaag ggttggttcc ccaagtctta cgtgaaactc
3661 atttcagggc ccataaggaa gtctacaagc atggattctg gttcttcaga gagtcctgct
3721 agtctaaagc gagtagcctc tccagcagcc aagccggtcg tttcgggaga agaatttatt
3781 gccatgtaca cttacgagag ttctgagcaa ggagatttaa cctttcagca aggggatgtg
3841 attttggtta ccaagaaaga tggtgactgg tggacaggaa cagtgggcga caaggccgga
3901 gtcttccctt ctaactatgt gaggcttaaa gattcagagg gctctggaac tgctgggaaa
3961 acagggagtt taggaaaaaa acctgaaatt gcccaggtta ttgcctcata caccgccacc
4021 ggccccgagc agctcactct cgcccctggt cagctgattt tgatccgaaa aaagaaccca
4081 ggtggatggt gggaaggaga gctgcaagca cgtgggaaaa agcgccagat aggctggttc
4141 ccagctaatt atgtaaagct tctaagccct gggacgagca aaatcactcc aacagagcca
4201 cctaagtcaa cagcattagc ggcagtgtgc caggtaagta tcaaggttac aagacaggtt
4261 taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta ttaagttatg
4321 gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
4381 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
4441 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
4501 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
4561 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
4621 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
4681 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
4741 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
4801 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
4861 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
4921 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
4981 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
5041 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
5101 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
5161 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
5221 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
5281 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
5341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
5401 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
5461 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
5521 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
5581 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
5641 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
5701 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
5761 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
5821 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
5881 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
5941 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
6001 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
6061 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
6121 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
6181 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
6241 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
6301 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
6361 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
6421 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
6481 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
6541 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
6601 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
6661 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
6721 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
6781 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
6841 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
6901 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
6961 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
7021 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
7081 tttgctcaca tgt
//
